Detection of HPV-2 and identification of novel mutations by whole genome sequencing from biopsies of two patients with multiple cutaneous horns

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Wei Wang
  • Chen Wang
  • Shuqing Xu
  • Chen Chen
  • Xiaomei Tong
  • Yu Liang
  • Xiaoping Dong
  • Yanjun Lei
  • Xiaoguang Zheng
  • Jun Yu
  • Jian Wang
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000248631300006&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.jcv.2007.01.002
eISSN
1873-5967
Externe Identifier
  • Clarivate Analytics Document Solution ID: 198MD
  • PubMed Identifier: 17368088
ISSN
1386-6532
Ausgabe der Veröffentlichung
1
Zeitschrift
JOURNAL OF CLINICAL VIROLOGY
Schlüsselwörter
  • HPV-2
  • cutaneous horns
  • PCR
  • sequencing
  • genome
  • mutation
Paginierung
34 - 42
Datum der Veröffentlichung
2007
Status
Published
Titel
Detection of HPV-2 and identification of novel mutations by whole genome sequencing from biopsies of two patients with multiple cutaneous horns
Sub types
  • Article
Ausgabe der Zeitschrift
39

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Autoren
  • Wei Wang
  • Chen Wang
  • Shuqing Xu
  • Chen Chen
  • Xiaomei Tong
  • Yu Liang
  • Xiaoping Dong
  • Yanjun Lei
  • Xiaoguang Zheng
  • Jun Yu
  • Jian Wang
DOI
10.1016/j.jcv.2007.01.002
ISSN
1386-6532
Ausgabe der Veröffentlichung
1
Zeitschrift
Journal of Clinical Virology
Sprache
en
Paginierung
34 - 42
Datum der Veröffentlichung
2007
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jcv.2007.01.002
Datum der Datenerfassung
2020
Titel
Detection of HPV-2 and identification of novel mutations by whole genome sequencing from biopsies of two patients with multiple cutaneous horns
Ausgabe der Zeitschrift
39

Datenquelle: Crossref

Abstract
<h4>Background</h4>Human papillomavirus-2 (HPV-2) is generally associated with common warts. The association of cutaneous horns with HPV-2 infection has never been reported.<h4>Objectives</h4>To identify the papillomavirus (PV) type(s) involved in cutaneous horns and analysis the genomes of these viruses.<h4>Study design</h4>We screened biopsies from two patients with multiple cutaneous horns using PV type-specific PCR assays, and sequenced the whole genomes of the viruses by a PCR-by-PCR strategy. Sequence comparison with the reference genome and its closely related PVs in the same phylogenetic group was performed to identify sequence variation across the genome(s) of newly detected PV(s).<h4>Results</h4>Two strains of HPV-2 were identified from the biopsies of two patients respectively. No double or multiple infections were detected. Novel mutations were found in the HPV-2 genome, located both in the coding and non-coding regions. Amino acid changes occurred only in E1 and E7 ORFs. The two strains also shared several mutations at the same positions.<h4>Conclusions</h4>Each patient was infected with a single strain of HPV-2 that developed unique mutations; HPV-2 may play a role in the onset and development of cutaneous horns; amino acid changes in functionally significant viral proteins may confer differential pathogenic risks.
Addresses
  • Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China.
Autoren
  • Wei Wang
  • Chen Wang
  • Shuqing Xu
  • Chen Chen
  • Xiaomei Tong
  • Yu Liang
  • Xiaoping Dong
  • Yanjun Lei
  • Xiaoguang Zheng
  • Jun Yu
  • Jian Wang
DOI
10.1016/j.jcv.2007.01.002
eISSN
1873-5967
Externe Identifier
  • PubMed Identifier: 17368088
Open access
false
ISSN
1386-6532
Ausgabe der Veröffentlichung
1
Zeitschrift
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
Schlüsselwörter
  • Humans
  • Papillomaviridae
  • Papillomavirus Infections
  • Foot Dermatoses
  • Hand Dermatoses
  • Keratosis
  • Sequence Alignment
  • Phylogeny
  • Amino Acid Sequence
  • Mutation
  • Genome, Viral
  • Molecular Sequence Data
  • Adult
  • Middle Aged
  • Male
Sprache
eng
Medium
Print-Electronic
Online publication date
2007
Paginierung
34 - 42
Datum der Veröffentlichung
2007
Status
Published
Datum der Datenerfassung
2007
Titel
Detection of HPV-2 and identification of novel mutations by whole genome sequencing from biopsies of two patients with multiple cutaneous horns.
Sub types
  • Journal Article
  • Case Reports
Ausgabe der Zeitschrift
39

Datenquelle: Europe PubMed Central

Abstract
BACKGROUND: Human papillomavirus-2 (HPV-2) is generally associated with common warts. The association of cutaneous horns with HPV-2 infection has never been reported. OBJECTIVES: To identify the papillomavirus (PV) type(s) involved in cutaneous horns and analysis the genomes of these viruses. STUDY DESIGN: We screened biopsies from two patients with multiple cutaneous horns using PV type-specific PCR assays, and sequenced the whole genomes of the viruses by a PCR-by-PCR strategy. Sequence comparison with the reference genome and its closely related PVs in the same phylogenetic group was performed to identify sequence variation across the genome(s) of newly detected PV(s). RESULTS: Two strains of HPV-2 were identified from the biopsies of two patients respectively. No double or multiple infections were detected. Novel mutations were found in the HPV-2 genome, located both in the coding and non-coding regions. Amino acid changes occurred only in E1 and E7 ORFs. The two strains also shared several mutations at the same positions. CONCLUSIONS: Each patient was infected with a single strain of HPV-2 that developed unique mutations; HPV-2 may play a role in the onset and development of cutaneous horns; amino acid changes in functionally significant viral proteins may confer differential pathogenic risks.
Date of acceptance
2007
Autoren
  • Wei Wang
  • Chen Wang
  • Shuqing Xu
  • Chen Chen
  • Xiaomei Tong
  • Yu Liang
  • Xiaoping Dong
  • Yanjun Lei
  • Xiaoguang Zheng
  • Jun Yu
  • Jian Wang
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/17368088
DOI
10.1016/j.jcv.2007.01.002
ISSN
1386-6532
Ausgabe der Veröffentlichung
1
Zeitschrift
J Clin Virol
Schlüsselwörter
  • Adult
  • Amino Acid Sequence
  • Foot Dermatoses
  • Genome, Viral
  • Hand Dermatoses
  • Humans
  • Keratosis
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Mutation
  • Papillomaviridae
  • Papillomavirus Infections
  • Phylogeny
  • Sequence Alignment
Sprache
eng
Country
Netherlands
Paginierung
34 - 42
PII
S1386-6532(07)00027-3
Datum der Veröffentlichung
2007
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2007
Titel
Detection of HPV-2 and identification of novel mutations by whole genome sequencing from biopsies of two patients with multiple cutaneous horns.
Sub types
  • Case Reports
  • Journal Article
Ausgabe der Zeitschrift
39

Datenquelle: PubMed

Beziehungen:
Eigentum von

Werkzeuge