ZEITLUPE facilitates the rhythmic movements of Nicotiana attenuata flowers

Abstract

SUMMARYCircadian organ movements are ubiquitous in plants. These rhythmic outputs are thought to be regulated by the circadian clock and auxin signalling, but the underlying mechanisms have not been clarified. Flowers of Nicotiana attenuata change their orientation during the daytime through a 140° arc to balance the need for pollinators and the protection of their reproductive organs. This rhythmic trait is under the control of the circadian clock and results from bending and re‐straightening movements of the pedicel, stems that connect flowers to the inflorescence. Using an explant system that allowed pedicel growth and curvature responses to be characterized with high spatial and temporal resolution, we demonstrated that this movement is organ autonomous and mediated by auxin. Changes in the growth curvature of the pedicel are accompanied by an auxin gradient and dorsiventral asymmetry in auxin‐dependent transcriptional responses; application of auxin transport inhibitors influenced the normal movements of this organ. Silencing the expression of the circadian clock component ZEITLUPE (ZTL) arrested changes in the growth curvature of the pedicel and altered auxin signalling and responses. IAA19‐like, an Aux/IAA transcriptional repressor that is circadian regulated and differentially expressed between opposite tissues of the pedicel, and therefore possibly involved in the regulation of changes in organ curvature, physically interacted with ZTL. Together, these results are consistent with a direct link between the circadian clock and the auxin signalling pathway in the regulation of this rhythmic floral movement.

Autoren

Lucas Cortés Llorca
Ran Li
Felipe Yon
Martin Schäfer
Rayko Halitschke
Christelle AM Robert
Sang‐Gyu Kim
Ian T Baldwin

DOI

10.1111/tpj.14732

eISSN

1365-313X

ISSN

0960-7412

Ausgabe der Veröffentlichung

1

Zeitschrift

The Plant Journal

Sprache

en

Online publication date

2020

Paginierung

308 - 322

Datum der Veröffentlichung

2020

Status

Published

Herausgeber

Wiley

Herausgeber URL

http://dx.doi.org/10.1111/tpj.14732

Datum der Datenerfassung

2023

Titel

ZEITLUPE facilitates the rhythmic movements of <i>Nicotiana attenuata</i> flowers

Ausgabe der Zeitschrift

103

Datenquelle: Crossref

Abstract

Circadian organ movements are ubiquitous in plants. These rhythmic outputs are thought to be regulated by the circadian clock and auxin signalling, but the underlying mechanisms have not been clarified. Flowers of Nicotiana attenuata change their orientation during the daytime through a 140° arc to balance the need for pollinators and the protection of their reproductive organs. This rhythmic trait is under the control of the circadian clock and results from bending and re-straightening movements of the pedicel, stems that connect flowers to the inflorescence. Using an explant system that allowed pedicel growth and curvature responses to be characterized with high spatial and temporal resolution, we demonstrated that this movement is organ autonomous and mediated by auxin. Changes in the growth curvature of the pedicel are accompanied by an auxin gradient and dorsiventral asymmetry in auxin-dependent transcriptional responses; application of auxin transport inhibitors influenced the normal movements of this organ. Silencing the expression of the circadian clock component ZEITLUPE (ZTL) arrested changes in the growth curvature of the pedicel and altered auxin signalling and responses. IAA19-like, an Aux/IAA transcriptional repressor that is circadian regulated and differentially expressed between opposite tissues of the pedicel, and therefore possibly involved in the regulation of changes in organ curvature, physically interacted with ZTL. Together, these results are consistent with a direct link between the circadian clock and the auxin signalling pathway in the regulation of this rhythmic floral movement.

Addresses

Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, 007745, Germany.

Autoren

Lucas Cortés Llorca
Ran Li
Felipe Yon
Martin Schäfer
Rayko Halitschke
Christelle AM Robert
Sang-Gyu Kim
Ian T Baldwin

DOI

10.1111/tpj.14732

eISSN

1365-313X

Externe Identifier

PubMed Identifier: 32130751

Funding acknowledgements

European Research Council: 293926
Rural Development Administration:

Open access

false

ISSN

0960-7412

Ausgabe der Veröffentlichung

1

Zeitschrift

The Plant journal : for cell and molecular biology

Schlüsselwörter

Flowers
Indoleacetic Acids
Plant Growth Regulators
Plant Proteins
Gene Expression Regulation, Plant
Circadian Rhythm
Circadian Rhythm Signaling Peptides and Proteins
Nicotiana

Sprache

eng

Medium

Print-Electronic

Online publication date

2020

Paginierung

308 - 322

Datum der Veröffentlichung

2020

Status

Published

Publisher licence

CC BY-NC

Datum der Datenerfassung

2020

Titel

ZEITLUPE facilitates the rhythmic movements of Nicotiana attenuata flowers.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

103

Datenquelle: Europe PubMed Central

Abstract

Circadian organ movements are ubiquitous in plants. These rhythmic outputs are thought to be regulated by the circadian clock and auxin signalling, but the underlying mechanisms have not been clarified. Flowers of Nicotiana attenuata change their orientation during the daytime through a 140° arc to balance the need for pollinators and the protection of their reproductive organs. This rhythmic trait is under the control of the circadian clock and results from bending and re-straightening movements of the pedicel, stems that connect flowers to the inflorescence. Using an explant system that allowed pedicel growth and curvature responses to be characterized with high spatial and temporal resolution, we demonstrated that this movement is organ autonomous and mediated by auxin. Changes in the growth curvature of the pedicel are accompanied by an auxin gradient and dorsiventral asymmetry in auxin-dependent transcriptional responses; application of auxin transport inhibitors influenced the normal movements of this organ. Silencing the expression of the circadian clock component ZEITLUPE (ZTL) arrested changes in the growth curvature of the pedicel and altered auxin signalling and responses. IAA19-like, an Aux/IAA transcriptional repressor that is circadian regulated and differentially expressed between opposite tissues of the pedicel, and therefore possibly involved in the regulation of changes in organ curvature, physically interacted with ZTL. Together, these results are consistent with a direct link between the circadian clock and the auxin signalling pathway in the regulation of this rhythmic floral movement.

Date of acceptance

2020

Autoren

Lucas Cortés Llorca
Ran Li
Felipe Yon
Martin Schäfer
Rayko Halitschke
Christelle AM Robert
Sang-Gyu Kim
Ian T Baldwin

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/32130751

DOI

10.1111/tpj.14732

eISSN

1365-313X

Funding acknowledgements

European Research Council: 293926

Ausgabe der Veröffentlichung

1

Zeitschrift

Plant J

Schlüsselwörter

Nicotiana attenuata
auxin signalling
circadian clock
plant movement
Circadian Rhythm
Circadian Rhythm Signaling Peptides and Proteins
Flowers
Gene Expression Regulation, Plant
Indoleacetic Acids
Plant Growth Regulators
Plant Proteins
Nicotiana

Sprache

eng

Country

England

Paginierung

308 - 322

Datum der Veröffentlichung

2020

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2021

Titel

ZEITLUPE facilitates the rhythmic movements of Nicotiana attenuata flowers.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

103

Datenquelle: PubMed

ZEITLUPE facilitates the rhythmic movements of Nicotiana attenuata flowers

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