'Real time' genetic manipulation: a new tool for ecological field studies

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Martin Schaefer
  • Christoph Bruetting
  • Klaus Gase
  • Michael Reichelt
  • Ian Baldwin
  • Stefan Meldau
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000326246700013&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1111/tpj.12301
eISSN
1365-313X
Externe Identifier
  • Clarivate Analytics Document Solution ID: 242MP
  • PubMed Identifier: 23906159
ISSN
0960-7412
Ausgabe der Veröffentlichung
3
Zeitschrift
PLANT JOURNAL
Schlüsselwörter
  • pOp6
  • LhGR
  • dexamethasone
  • fieldwork
  • Nicotiana attenuata
  • Tupiocoris notatus
  • cytokinin
  • Manduca sexta
  • herbivory
  • pds
  • technical advance
Paginierung
506 - 518
Datum der Veröffentlichung
2013
Status
Published
Titel
'Real time' genetic manipulation: a new tool for ecological field studies
Sub types
  • Article
Ausgabe der Zeitschrift
76

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<jats:title>Summary</jats:title><jats:p>Field experiments with transgenic plants often reveal the functional significance of genetic traits that are important for the performance of the plants in their natural environments. Until now, only constitutive overexpression, ectopic expression and gene silencing methods have been used to analyze gene‐related phenotypes in natural habitats. These methods do not allow sufficient control over gene expression for the study of ecological interactions in real time, of genetic traits that play essential roles in development, or of dose‐dependent effects. We applied the sensitive dexamethasone (<jats:styled-content style="fixed-case">DEX</jats:styled-content>)‐inducible p<jats:styled-content style="fixed-case">O</jats:styled-content>p6/<jats:styled-content style="fixed-case">L</jats:styled-content>h<jats:styled-content style="fixed-case">GR</jats:styled-content> expression system to the ecological model plant <jats:italic><jats:styled-content style="fixed-case">N</jats:styled-content>icotiana attenuata</jats:italic> and established a lanolin‐based <jats:styled-content style="fixed-case">DEX</jats:styled-content> application method to facilitate ectopic gene expression and <jats:styled-content style="fixed-case">RNA</jats:styled-content> interference‐mediated gene silencing in the field and under challenging conditions (e.g. high temperature, wind and <jats:styled-content style="fixed-case">UV</jats:styled-content> radiation). Fully established field‐grown plants were used to silence phytoene desaturase and thereby cause photobleaching only in specific plant sectors, and to activate expression of the cytokinin (<jats:styled-content style="fixed-case">CK</jats:styled-content>) biosynthesis gene isopentenyl transferase (<jats:styled-content style="fixed-case"><jats:italic>ipt</jats:italic></jats:styled-content>). We used <jats:styled-content style="fixed-case"><jats:italic>ipt</jats:italic></jats:styled-content> expression to analyze the role of <jats:styled-content style="fixed-case">CK</jats:styled-content>s in both the glasshouse and the field to understand resistance to the native herbivore <jats:italic><jats:styled-content style="fixed-case">T</jats:styled-content>upiocoris notatus</jats:italic>, which attacks plants at small spatial scales. By spatially restricting <jats:italic>ipt</jats:italic> expression and elevating <jats:styled-content style="fixed-case">CK</jats:styled-content> levels in single leaves, damage by <jats:italic><jats:styled-content style="fixed-case">T</jats:styled-content>. notatus</jats:italic> increased, demonstrating the role of <jats:styled-content style="fixed-case">CK</jats:styled-content>s in this plant–herbivore interaction at a small scale. As the arena of most ecological interactions is highly constrained in time and space, these tools will advance the genetic analysis of dynamic traits that matter for plant performance in nature.</jats:p>
Autoren
  • Martin Schäfer
  • Christoph Brütting
  • Klaus Gase
  • Michael Reichelt
  • Ian Baldwin
  • Stefan Meldau
DOI
10.1111/tpj.12301
eISSN
1365-313X
ISSN
0960-7412
Ausgabe der Veröffentlichung
3
Zeitschrift
The Plant Journal
Sprache
en
Online publication date
2013
Paginierung
506 - 518
Datum der Veröffentlichung
2013
Status
Published
Herausgeber
Wiley
Herausgeber URL
http://dx.doi.org/10.1111/tpj.12301
Datum der Datenerfassung
2023
Titel
‘Real time’ genetic manipulation: a new tool for ecological field studies
Ausgabe der Zeitschrift
76

Datenquelle: Crossref

Abstract
Field experiments with transgenic plants often reveal the functional significance of genetic traits that are important for the performance of the plants in their natural environments. Until now, only constitutive overexpression, ectopic expression and gene silencing methods have been used to analyze gene-related phenotypes in natural habitats. These methods do not allow sufficient control over gene expression for the study of ecological interactions in real time, of genetic traits that play essential roles in development, or of dose-dependent effects. We applied the sensitive dexamethasone (DEX)-inducible pOp6/LhGR expression system to the ecological model plant Nicotiana attenuata and established a lanolin-based DEX application method to facilitate ectopic gene expression and RNA interference-mediated gene silencing in the field and under challenging conditions (e.g. high temperature, wind and UV radiation). Fully established field-grown plants were used to silence phytoene desaturase and thereby cause photobleaching only in specific plant sectors, and to activate expression of the cytokinin (CK) biosynthesis gene isopentenyl transferase (ipt). We used ipt expression to analyze the role of CKs in both the glasshouse and the field to understand resistance to the native herbivore Tupiocoris notatus, which attacks plants at small spatial scales. By spatially restricting ipt expression and elevating CK levels in single leaves, damage by T. notatus increased, demonstrating the role of CKs in this plant-herbivore interaction at a small scale. As the arena of most ecological interactions is highly constrained in time and space, these tools will advance the genetic analysis of dynamic traits that matter for plant performance in nature.
Addresses
  • Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans Knöll Strasse 8, Jena, 07745, Germany.
Autoren
  • Martin Schäfer
  • Christoph Brütting
  • Klaus Gase
  • Michael Reichelt
  • Ian Baldwin
  • Stefan Meldau
DOI
10.1111/tpj.12301
eISSN
1365-313X
Externe Identifier
  • PubMed Identifier: 23906159
  • PubMed Central ID: PMC4190501
Funding acknowledgements
  • European Research Council: 293926
Open access
false
ISSN
0960-7412
Ausgabe der Veröffentlichung
3
Zeitschrift
The Plant journal : for cell and molecular biology
Schlüsselwörter
  • Animals
  • Plants, Genetically Modified
  • Dexamethasone
  • Genetic Techniques
  • Ecology
  • RNA Interference
  • Transgenes
  • Herbivory
  • Nicotiana
Sprache
eng
Medium
Print-Electronic
Online publication date
2013
Paginierung
506 - 518
Datum der Veröffentlichung
2013
Status
Published
Datum der Datenerfassung
2013
Titel
'Real time' genetic manipulation: a new tool for ecological field studies.
Sub types
  • Research Support, Non-U.S. Gov't
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
76

Files

https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/tpj.12301 https://europepmc.org/articles/PMC4190501?pdf=render

Datenquelle: Europe PubMed Central

Abstract
Field experiments with transgenic plants often reveal the functional significance of genetic traits that are important for the performance of the plants in their natural environments. Until now, only constitutive overexpression, ectopic expression and gene silencing methods have been used to analyze gene-related phenotypes in natural habitats. These methods do not allow sufficient control over gene expression for the study of ecological interactions in real time, of genetic traits that play essential roles in development, or of dose-dependent effects. We applied the sensitive dexamethasone (DEX)-inducible pOp6/LhGR expression system to the ecological model plant Nicotiana attenuata and established a lanolin-based DEX application method to facilitate ectopic gene expression and RNA interference-mediated gene silencing in the field and under challenging conditions (e.g. high temperature, wind and UV radiation). Fully established field-grown plants were used to silence phytoene desaturase and thereby cause photobleaching only in specific plant sectors, and to activate expression of the cytokinin (CK) biosynthesis gene isopentenyl transferase (ipt). We used ipt expression to analyze the role of CKs in both the glasshouse and the field to understand resistance to the native herbivore Tupiocoris notatus, which attacks plants at small spatial scales. By spatially restricting ipt expression and elevating CK levels in single leaves, damage by T. notatus increased, demonstrating the role of CKs in this plant-herbivore interaction at a small scale. As the arena of most ecological interactions is highly constrained in time and space, these tools will advance the genetic analysis of dynamic traits that matter for plant performance in nature.
Date of acceptance
2013
Autoren
  • Martin Schäfer
  • Christoph Brütting
  • Klaus Gase
  • Michael Reichelt
  • Ian Baldwin
  • Stefan Meldau
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/23906159
DOI
10.1111/tpj.12301
eISSN
1365-313X
Externe Identifier
  • NIH Manuscript Submission ID: EMS57848
  • PubMed Central ID: PMC4190501
Funding acknowledgements
  • European Research Council: 293926
Ausgabe der Veröffentlichung
3
Zeitschrift
Plant J
Schlüsselwörter
  • LhGR
  • Manduca sexta
  • Nicotiana attenuata
  • Tupiocoris notatus
  • cytokinin
  • dexamethasone
  • fieldwork
  • herbivory
  • pOp6
  • pds
  • technical advance
  • Animals
  • Dexamethasone
  • Ecology
  • Genetic Techniques
  • Herbivory
  • Plants, Genetically Modified
  • RNA Interference
  • Nicotiana
  • Transgenes
Sprache
eng
Country
England
Paginierung
506 - 518
Datum der Veröffentlichung
2013
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2014
Titel
'Real time' genetic manipulation: a new tool for ecological field studies.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
76

Datenquelle: PubMed

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