Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi

Abstract

High-through-put (HTP) screening for functional arbuscular mycorrhizal fungi (AMF)-associations is challenging because roots must be excavated and colonization evaluated by transcript analysis or microscopy. Here we show that specific leaf-metabolites provide broadly applicable accurate proxies of these associations, suitable for HTP-screens. With a combination of untargeted and targeted metabolomics, we show that shoot accumulations of hydroxy- and carboxyblumenol C-glucosides mirror root AMF-colonization in Nicotiana attenuata plants. Genetic/pharmacologic manipulations indicate that these AMF-indicative foliar blumenols are synthesized and transported from roots to shoots. These blumenol-derived foliar markers, found in many di- and monocotyledonous crop and model plants (Solanum lycopersicum, Solanum tuberosum, Hordeum vulgare, Triticum aestivum, Medicago truncatula and Brachypodium distachyon), are not restricted to particular plant-AMF interactions, and are shown to be applicable for field-based QTL mapping of AMF-related genes.

Autoren

Ming Wang
Martin Schäfer
Dapeng Li
Rayko Halitschke
Chuanfu Dong
Erica McGale
Christian Paetz
Yuanyuan Song
Suhua Li
Junfu Dong
Sven Heiling
Karin Groten
Philipp Franken
Michael Bitterlich
Maria J Harrison
Uta Paszkowski
Ian T Baldwin

DOI

10.7554/elife.37093

eISSN

2050-084X

Zeitschrift

eLife

Sprache

en

Online publication date

2018

Status

Published online

Herausgeber

eLife Sciences Publications, Ltd

Herausgeber URL

http://dx.doi.org/10.7554/elife.37093

Datum der Datenerfassung

2023

Titel

Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi

Ausgabe der Zeitschrift

7

Datenquelle: Crossref

Abstract

High-through-put (HTP) screening for functional arbuscular mycorrhizal fungi (AMF)-associations is challenging because roots must be excavated and colonization evaluated by transcript analysis or microscopy. Here we show that specific leaf-metabolites provide broadly applicable accurate proxies of these associations, suitable for HTP-screens. With a combination of untargeted and targeted metabolomics, we show that shoot accumulations of hydroxy- and carboxyblumenol C-glucosides mirror root AMF-colonization in Nicotiana attenuata plants. Genetic/pharmacologic manipulations indicate that these AMF-indicative foliar blumenols are synthesized and transported from roots to shoots. These blumenol-derived foliar markers, found in many di- and monocotyledonous crop and model plants (Solanum lycopersicum, Solanum tuberosum, Hordeum vulgare, Triticum aestivum, Medicago truncatula and Brachypodium distachyon), are not restricted to particular plant-AMF interactions, and are shown to be applicable for field-based QTL mapping of AMF-related genes.

Addresses

Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany.

Autoren

Ming Wang
Martin Schäfer
Dapeng Li
Rayko Halitschke
Chuanfu Dong
Erica McGale
Christian Paetz
Yuanyuan Song
Suhua Li
Junfu Dong
Sven Heiling
Karin Groten
Philipp Franken
Michael Bitterlich
Maria J Harrison
Uta Paszkowski
Ian T Baldwin

DOI

10.7554/elife.37093

eISSN

2050-084X

Externe Identifier

PubMed Identifier: 30152755
PubMed Central ID: PMC6156081

Funding acknowledgements

Biotechnology and Biological Sciences Research Council: BB/N008723/1
Biotechnology and Biological Sciences Research Council: BB/P003419/1
Ministry of Consumer Protection, Food and Agriculture of the Federal Republic of Germany:
European Research Council: 293926
European Innovation Partnership Agricultural Productivity and Sustainability: 276033540220041
Elsa-Neumann Scholarship:
Ministry of Science, Research and Culture of the State of Brandenburg:
European Research Council: Advanced Grant ClockworkGreen (293926)
Max-Planck-Gesellschaft: Open-access funding
U.S. Department of Energy: # DESC0012460
European Innovation Partnership Agri: 276033540220041
European Research Council: ClockworkGreen (293926)
Thuringian Ministry for Infrastructure and Agriculture:

Open access

true

ISSN

2050-084X

Zeitschrift

eLife

Schlüsselwörter

Mycorrhizae
Plant Leaves
Plant Shoots
Cyclohexanones
RNA, Messenger
Symbiosis
Genes, Plant
Time Factors
Stress, Physiological
Metabolomics
High-Throughput Screening Assays
Biomarkers
Nicotiana

Sprache

eng

Medium

Electronic

Online publication date

2018

Open access status

Open Access

Paginierung

e37093

Datum der Veröffentlichung

2018

Status

Published

Publisher licence

CC BY

Datum der Datenerfassung

2018

Titel

Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi.

Sub types

Research Support, Non-U.S. Gov't
research-article
Research Support, U.S. Gov't, Non-P.H.S.
Journal Article

Ausgabe der Zeitschrift

7

Files

https://europepmc.org/articles/PMC6156081?pdf=render

Datenquelle: Europe PubMed Central

Abstract

High-through-put (HTP) screening for functional arbuscular mycorrhizal fungi (AMF)-associations is challenging because roots must be excavated and colonization evaluated by transcript analysis or microscopy. Here we show that specific leaf-metabolites provide broadly applicable accurate proxies of these associations, suitable for HTP-screens. With a combination of untargeted and targeted metabolomics, we show that shoot accumulations of hydroxy- and carboxyblumenol C-glucosides mirror root AMF-colonization in Nicotiana attenuata plants. Genetic/pharmacologic manipulations indicate that these AMF-indicative foliar blumenols are synthesized and transported from roots to shoots. These blumenol-derived foliar markers, found in many di- and monocotyledonous crop and model plants (Solanum lycopersicum, Solanum tuberosum, Hordeum vulgare, Triticum aestivum, Medicago truncatula and Brachypodium distachyon), are not restricted to particular plant-AMF interactions, and are shown to be applicable for field-based QTL mapping of AMF-related genes.

Date of acceptance

2018

Autoren

Ming Wang
Martin Schäfer
Dapeng Li
Rayko Halitschke
Chuanfu Dong
Erica McGale
Christian Paetz
Yuanyuan Song
Suhua Li
Junfu Dong
Sven Heiling
Karin Groten
Philipp Franken
Michael Bitterlich
Maria J Harrison
Uta Paszkowski
Ian T Baldwin

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/30152755

DOI

10.7554/eLife.37093

eISSN

2050-084X

Externe Identifier

PubMed Central ID: PMC6156081

Funding acknowledgements

Biotechnology and Biological Sciences Research Council: BB/N008723/1
European Innovation Partnership Agri: 276033540220041
European Innovation Partnership Agricultural Productivity and Sustainability: 276033540220041
European Research Council: 293926
European Research Council: Advanced Grant ClockworkGreen (293926)
Biotechnology and Biological Sciences Research Council: BB/P003419/1
U.S. Department of Energy: # DESC0012460
ERC Advanced Grant: ClockworkGreen (293926)
Max-Planck-Gesellschaft: Open-access funding

Zeitschrift

Elife

Schlüsselwörter

Nicotiana attenuata
Rhizophagus irregularis
arbuscular mycorrhizal fungi
blumenol
colonization rate
ecology
high-throughput screening
plant biology
Biomarkers
Cyclohexanones
Genes, Plant
High-Throughput Screening Assays
Metabolomics
Mycorrhizae
Plant Leaves
Plant Shoots
RNA, Messenger
Stress, Physiological
Symbiosis
Time Factors
Nicotiana

Sprache

eng

Country

England

PII

37093

Datum der Veröffentlichung

2018

Status

Published online

Datum, an dem der Datensatz öffentlich gemacht wurde

2019

Titel

Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi.

Sub types

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Ausgabe der Zeitschrift

7

Datenquelle: PubMed

Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi

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