ARABIDOMICS-A new experimental platform for molecular analyses of plants in drop towers, on parabolic flights, and sounding rockets

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Jens Hauslage
  • Mark Goeroeg
  • Lars Krause
  • Oliver Schueler
  • Martin Schaefer
  • Anika Witten
  • Leona Kesseler
  • Maik Boehmer
  • Ruth Hemmersbach
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000522052300001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1063/1.5120573
eISSN
1089-7623
Externe Identifier
  • Clarivate Analytics Document Solution ID: KX7IZ
  • PubMed Identifier: 32259966
ISSN
0034-6748
Ausgabe der Veröffentlichung
3
Zeitschrift
REVIEW OF SCIENTIFIC INSTRUMENTS
Artikelnummer
ARTN 034504
Datum der Veröffentlichung
2020
Status
Published
Titel
ARABIDOMICS-A new experimental platform for molecular analyses of plants in drop towers, on parabolic flights, and sounding rockets
Sub types
  • Article
Ausgabe der Zeitschrift
91

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<jats:p>Plants represent an essential part of future life support systems that will enable human space travel to distant planets and their colonization. Therefore, insights into changes and adaptations of plants in microgravity are of great importance. Despite considerable efforts, we still know very little about how plants respond to microgravity environments on the molecular level, partly due to a lack of sufficient hardware and flight opportunities. The plant Arabidopsis thaliana, the subject of this study, represents a well-studied model organism in gravitational biology, particularly for the analysis of transcriptional and metabolic changes. To overcome the limitations of previous plant hardware that often led to secondary effects and to allow for the extraction not only of RNA but also of phytohormones and proteins, we developed a new experimental platform, called ARABIDOMICS, for exposure and fixation under altered gravity conditions. Arabidopsis seedlings were exposed to hypergravity during launch and microgravity during the free-fall period of the MAPHEUS 5 sounding rocket. Seedlings were chemically fixed inflight at defined time points, and RNA and phytohormones were subsequently analyzed in the laboratory. RNA and phytohormones extracted from the fixed biological samples were of excellent quality. Changes in the phytohormone content of jasmonate, auxin, and several cytokinins were observed in response to hypergravity and microgravity.</jats:p>
Autoren
  • Jens Hauslage
  • Mark Görög
  • Lars Krause
  • Oliver Schüler
  • Martin Schäfer
  • Anika Witten
  • Leona Kesseler
  • Maik Böhmer
  • Ruth Hemmersbach
DOI
10.1063/1.5120573
eISSN
1089-7623
ISSN
0034-6748
Ausgabe der Veröffentlichung
3
Zeitschrift
Review of Scientific Instruments
Sprache
en
Online publication date
2020
Datum der Veröffentlichung
2020
Status
Published
Herausgeber
AIP Publishing
Herausgeber URL
http://dx.doi.org/10.1063/1.5120573
Datum der Datenerfassung
2023
Titel
ARABIDOMICS—A new experimental platform for molecular analyses of plants in drop towers, on parabolic flights, and sounding rockets
Ausgabe der Zeitschrift
91

Datenquelle: Crossref

Abstract
Plants represent an essential part of future life support systems that will enable human space travel to distant planets and their colonization. Therefore, insights into changes and adaptations of plants in microgravity are of great importance. Despite considerable efforts, we still know very little about how plants respond to microgravity environments on the molecular level, partly due to a lack of sufficient hardware and flight opportunities. The plant Arabidopsis thaliana, the subject of this study, represents a well-studied model organism in gravitational biology, particularly for the analysis of transcriptional and metabolic changes. To overcome the limitations of previous plant hardware that often led to secondary effects and to allow for the extraction not only of RNA but also of phytohormones and proteins, we developed a new experimental platform, called ARABIDOMICS, for exposure and fixation under altered gravity conditions. Arabidopsis seedlings were exposed to hypergravity during launch and microgravity during the free-fall period of the MAPHEUS 5 sounding rocket. Seedlings were chemically fixed inflight at defined time points, and RNA and phytohormones were subsequently analyzed in the laboratory. RNA and phytohormones extracted from the fixed biological samples were of excellent quality. Changes in the phytohormone content of jasmonate, auxin, and several cytokinins were observed in response to hypergravity and microgravity.
Addresses
  • Gravitational Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), 51147 Cologne, Germany.
Autoren
  • Jens Hauslage
  • Mark Görög
  • Lars Krause
  • Oliver Schüler
  • Martin Schäfer
  • Anika Witten
  • Leona Kesseler
  • Maik Böhmer
  • Ruth Hemmersbach
DOI
10.1063/1.5120573
eISSN
1089-7623
Externe Identifier
  • PubMed Identifier: 32259966
Funding acknowledgements
  • European Space Agency: 4000109583ESA-CORA-GBF-2013-005-BÖHMER&amp;
  • Bundesministerium für Wirtschaft und Energie: 50WB1830
Open access
false
ISSN
0034-6748
Ausgabe der Veröffentlichung
3
Zeitschrift
The Review of scientific instruments
Schlüsselwörter
  • Arabidopsis
  • Phytochrome
  • RNA, Plant
  • Hypergravity
  • Weightlessness
  • Space Flight
  • Seedlings
Sprache
eng
Medium
Print
Paginierung
034504
Datum der Veröffentlichung
2020
Status
Published
Datum der Datenerfassung
2020
Titel
ARABIDOMICS-A new experimental platform for molecular analyses of plants in drop towers, on parabolic flights, and sounding rockets.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
91

Datenquelle: Europe PubMed Central

Abstract
Plants represent an essential part of future life support systems that will enable human space travel to distant planets and their colonization. Therefore, insights into changes and adaptations of plants in microgravity are of great importance. Despite considerable efforts, we still know very little about how plants respond to microgravity environments on the molecular level, partly due to a lack of sufficient hardware and flight opportunities. The plant Arabidopsis thaliana, the subject of this study, represents a well-studied model organism in gravitational biology, particularly for the analysis of transcriptional and metabolic changes. To overcome the limitations of previous plant hardware that often led to secondary effects and to allow for the extraction not only of RNA but also of phytohormones and proteins, we developed a new experimental platform, called ARABIDOMICS, for exposure and fixation under altered gravity conditions. Arabidopsis seedlings were exposed to hypergravity during launch and microgravity during the free-fall period of the MAPHEUS 5 sounding rocket. Seedlings were chemically fixed inflight at defined time points, and RNA and phytohormones were subsequently analyzed in the laboratory. RNA and phytohormones extracted from the fixed biological samples were of excellent quality. Changes in the phytohormone content of jasmonate, auxin, and several cytokinins were observed in response to hypergravity and microgravity.
Autoren
  • Jens Hauslage
  • Mark Görög
  • Lars Krause
  • Oliver Schüler
  • Martin Schäfer
  • Anika Witten
  • Leona Kesseler
  • Maik Böhmer
  • Ruth Hemmersbach
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/32259966
DOI
10.1063/1.5120573
eISSN
1089-7623
Ausgabe der Veröffentlichung
3
Zeitschrift
Rev Sci Instrum
Schlüsselwörter
  • Arabidopsis
  • Hypergravity
  • Phytochrome
  • RNA, Plant
  • Seedlings
  • Space Flight
  • Weightlessness
Sprache
eng
Country
United States
Paginierung
034504
Datum der Veröffentlichung
2020
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2021
Titel
ARABIDOMICS-A new experimental platform for molecular analyses of plants in drop towers, on parabolic flights, and sounding rockets.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
91

Datenquelle: PubMed

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