Enzymatic Reactions Observed with Zero- and Low-Field Nuclear Magnetic Resonance
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- James Eills
- Roman Picazo-Frutos
- Oksana Bondar
- Eleonora Cavallari
- Carla Carrera
- Sylwia J Barker
- Marcel Utz
- Alba Herrero-Gomez
- Irene Marco-Rius
- Michael CD Tayler
- Silvio Aime
- Francesca Reineri
- Dmitry Budker
- John W Blanchard
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:001124560200001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1021/acs.analchem.3c02087
- eISSN
- 1520-6882
- Externe Identifier
- Clarivate Analytics Document Solution ID: CI2O1
- PubMed Identifier: 38047582
- ISSN
- 0003-2700
- Ausgabe der Veröffentlichung
- 49
- Zeitschrift
- ANALYTICAL CHEMISTRY
- Paginierung
- 17997 - 18005
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Titel
- Enzymatic Reactions Observed with Zero- and Low-Field Nuclear Magnetic Resonance
- Sub types
- Article
- Ausgabe der Zeitschrift
- 95
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- James Eills
- Román Picazo-Frutos
- Oksana Bondar
- Eleonora Cavallari
- Carla Carrera
- Sylwia J Barker
- Marcel Utz
- Alba Herrero-Gómez
- Irene Marco-Rius
- Michael CD Tayler
- Silvio Aime
- Francesca Reineri
- Dmitry Budker
- John W Blanchard
- DOI
- 10.1021/acs.analchem.3c02087
- eISSN
- 1520-6882
- ISSN
- 0003-2700
- Ausgabe der Veröffentlichung
- 49
- Zeitschrift
- Analytical Chemistry
- Sprache
- en
- Online publication date
- 2023
- Paginierung
- 17997 - 18005
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Herausgeber
- American Chemical Society (ACS)
- Herausgeber URL
- http://dx.doi.org/10.1021/acs.analchem.3c02087
- Datum der Datenerfassung
- 2024
- Titel
- Enzymatic Reactions Observed with Zero- and Low-Field Nuclear Magnetic Resonance
- Ausgabe der Zeitschrift
- 95
Datenquelle: Crossref
- Abstract
- We demonstrate that enzyme-catalyzed reactions can be observed in zero- and low-field NMR experiments by combining recent advances in parahydrogen-based hyperpolarization methods with state-of-the-art magnetometry. Specifically, we investigated two model biological processes: the conversion of fumarate into malate, which is used in vivo as a marker of cell necrosis, and the conversion of pyruvate into lactate, which is the most widely studied metabolic process in hyperpolarization-enhanced imaging. In addition to this, we constructed a microfluidic zero-field NMR setup to perform experiments on microliter-scale samples of [1-<sup>13</sup>C]fumarate in a lab-on-a-chip device. Zero- to ultralow-field (ZULF) NMR has two key advantages over high-field NMR: the signals can pass through conductive materials (e.g., metals), and line broadening from sample heterogeneity is negligible. To date, the use of ZULF NMR for process monitoring has been limited to studying hydrogenation reactions. In this work, we demonstrate this emerging analytical technique for more general reaction monitoring and compare zero- vs low-field detection.
- Addresses
- Barcelona Institute of Science and Technology, Institute for Bioengineering of Catalonia, Barcelona 08028, Spain.
- Autoren
- James Eills
- Román Picazo-Frutos
- Oksana Bondar
- Eleonora Cavallari
- Carla Carrera
- Sylwia J Barker
- Marcel Utz
- Alba Herrero-Gómez
- Irene Marco-Rius
- Michael CD Tayler
- Silvio Aime
- Francesca Reineri
- Dmitry Budker
- John W Blanchard
- DOI
- 10.1021/acs.analchem.3c02087
- eISSN
- 1520-6882
- Externe Identifier
- PubMed Identifier: 38047582
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: 465084791
- H2020 Future and Emerging Technologies: 858149
- H2020 Marie Sklodowska-Curie Actions: 101063517
- Engineering and Physical Sciences Research Council: EP/R513325/1
- Bruker BioSpin:
- H2020 Marie Sklodowska-Curie Actions: 766402
- Open access
- false
- ISSN
- 0003-2700
- Ausgabe der Veröffentlichung
- 49
- Zeitschrift
- Analytical chemistry
- Schlüsselwörter
- Fumarates
- Pyruvic Acid
- Magnetic Resonance Imaging
- Magnetic Resonance Spectroscopy
- Hydrogenation
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2023
- Paginierung
- 17997 - 18005
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Datum der Datenerfassung
- 2023
- Titel
- Enzymatic Reactions Observed with Zero- and Low-Field Nuclear Magnetic Resonance.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 95
Datenquelle: Europe PubMed Central
- Abstract
- We demonstrate that enzyme-catalyzed reactions can be observed in zero- and low-field NMR experiments by combining recent advances in parahydrogen-based hyperpolarization methods with state-of-the-art magnetometry. Specifically, we investigated two model biological processes: the conversion of fumarate into malate, which is used in vivo as a marker of cell necrosis, and the conversion of pyruvate into lactate, which is the most widely studied metabolic process in hyperpolarization-enhanced imaging. In addition to this, we constructed a microfluidic zero-field NMR setup to perform experiments on microliter-scale samples of [1-13C]fumarate in a lab-on-a-chip device. Zero- to ultralow-field (ZULF) NMR has two key advantages over high-field NMR: the signals can pass through conductive materials (e.g., metals), and line broadening from sample heterogeneity is negligible. To date, the use of ZULF NMR for process monitoring has been limited to studying hydrogenation reactions. In this work, we demonstrate this emerging analytical technique for more general reaction monitoring and compare zero- vs low-field detection.
- Autoren
- James Eills
- Román Picazo-Frutos
- Oksana Bondar
- Eleonora Cavallari
- Carla Carrera
- Sylwia J Barker
- Marcel Utz
- Alba Herrero-Gómez
- Irene Marco-Rius
- Michael CD Tayler
- Silvio Aime
- Francesca Reineri
- Dmitry Budker
- John W Blanchard
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/38047582
- DOI
- 10.1021/acs.analchem.3c02087
- eISSN
- 1520-6882
- Externe Identifier
- PubMed Central ID: PMC10720634
- Ausgabe der Veröffentlichung
- 49
- Zeitschrift
- Anal Chem
- Schlüsselwörter
- Magnetic Resonance Spectroscopy
- Magnetic Resonance Imaging
- Hydrogenation
- Pyruvic Acid
- Fumarates
- Sprache
- eng
- Country
- United States
- Paginierung
- 17997 - 18005
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2023
- Titel
- Enzymatic Reactions Observed with Zero- and Low-Field Nuclear Magnetic Resonance.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 95
Datenquelle: PubMed
- Beziehungen:
- Eigentum von