Amphiphilic Block Copolymers PEG-b-PMTCs: Synthesis, Self-Assembly, Degradation Properties and Biocompatibility

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Yiyi Deng
  • Sven Scha''fer
  • Devin Kronstein
  • Azra Atabay
  • Moritz Susewind
  • Elisha Krieg
  • Sebastian Seiffert
  • Jens Gaitzsch
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:001138348800001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1021/acs.biomac.3c00992
eISSN
1526-4602
Externe Identifier
  • Clarivate Analytics Document Solution ID: EI6Z9
  • PubMed Identifier: 38039186
ISSN
1525-7797
Ausgabe der Veröffentlichung
1
Zeitschrift
BIOMACROMOLECULES
Paginierung
303 - 314
Datum der Veröffentlichung
2023
Status
Published
Titel
Amphiphilic Block Copolymers PEG-<i>b</i>-PMTCs: Synthesis, Self-Assembly, Degradation Properties and Biocompatibility
Sub types
  • Article
Ausgabe der Zeitschrift
25

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Autoren
  • Yiyi Deng
  • Sven Schäfer
  • Devin Kronstein
  • Azra Atabay
  • Moritz Susewind
  • Elisha Krieg
  • Sebastian Seiffert
  • Jens Gaitzsch
DOI
10.1021/acs.biomac.3c00992
eISSN
1526-4602
ISSN
1525-7797
Ausgabe der Veröffentlichung
1
Zeitschrift
Biomacromolecules
Sprache
en
Online publication date
2023
Paginierung
303 - 314
Datum der Veröffentlichung
2024
Status
Published
Herausgeber
American Chemical Society (ACS)
Herausgeber URL
http://dx.doi.org/10.1021/acs.biomac.3c00992
Datum der Datenerfassung
2024
Titel
Amphiphilic Block Copolymers PEG-<i>b</i>-PMTCs: Synthesis, Self-Assembly, Degradation Properties and Biocompatibility
Ausgabe der Zeitschrift
25

Datenquelle: Crossref

Abstract
As a hydrophilic cyclic ketene acetal (CKA), 2-methylene-1,3,6-trioxocane (MTC) has recently attracted a lot of attention owing to its ability to promote a quicker (bio)degradation as compared to other heavily studied CKAs. Here, we prepared amphiphilic block copolymers based on poly-MTC with varying chain lengths by radical ring opening polymerization. Self-assemblies of these amphiphiles were performed in PBS buffer to generate nanoparticles with sizes from 40 to 105 nm, which were verified by dynamic light scattering, electron microscopy, and static light scattering (Zimm plots). Subsequently, fluorescence spectroscopy was applied to study the enzymatic degradation of Nile red-loaded nanoparticles. By performing a point-by-point comparison of fluorescence intensity decline patterns between nanoparticles, we demonstrated that lipase from <i>Pseudomonas cepacia</i> was very efficient in degrading the nanoparticles. Hydrolysis degradations under basic conditions were also carried out, and a complete degradation was achieved after 4 h. Additionally, cytotoxicity assays were carried out on HEK293 cells, and the results affirmed cell viabilities over 90% when incubated with up to 1 mg/mL nanoparticles for 24 h. These biodegradable and biocompatible nanoparticles hence hold great potential for future applications such as drug release.
Addresses
  • Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Straße 6, 01069 Dresden, Germany.
Autoren
  • Yiyi Deng
  • Sven Schäfer
  • Devin Kronstein
  • Azra Atabay
  • Moritz Susewind
  • Elisha Krieg
  • Sebastian Seiffert
  • Jens Gaitzsch
DOI
10.1021/acs.biomac.3c00992
eISSN
1526-4602
Externe Identifier
  • PubMed Identifier: 38039186
Funding acknowledgements
  • Deutsche Forschungsgemeinschaft: GA2051/7-1
  • China Scholarship Council:
Open access
false
ISSN
1525-7797
Ausgabe der Veröffentlichung
1
Zeitschrift
Biomacromolecules
Schlüsselwörter
  • Humans
  • Polyethylene Glycols
  • Polymers
  • Lipase
  • Hydrolysis
  • Micelles
  • HEK293 Cells
Sprache
eng
Medium
Print-Electronic
Online publication date
2023
Paginierung
303 - 314
Datum der Veröffentlichung
2024
Status
Published
Datum der Datenerfassung
2023
Titel
Amphiphilic Block Copolymers PEG-<i>b</i>-PMTCs: Synthesis, Self-Assembly, Degradation Properties and Biocompatibility.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
25

Datenquelle: Europe PubMed Central

Abstract
As a hydrophilic cyclic ketene acetal (CKA), 2-methylene-1,3,6-trioxocane (MTC) has recently attracted a lot of attention owing to its ability to promote a quicker (bio)degradation as compared to other heavily studied CKAs. Here, we prepared amphiphilic block copolymers based on poly-MTC with varying chain lengths by radical ring opening polymerization. Self-assemblies of these amphiphiles were performed in PBS buffer to generate nanoparticles with sizes from 40 to 105 nm, which were verified by dynamic light scattering, electron microscopy, and static light scattering (Zimm plots). Subsequently, fluorescence spectroscopy was applied to study the enzymatic degradation of Nile red-loaded nanoparticles. By performing a point-by-point comparison of fluorescence intensity decline patterns between nanoparticles, we demonstrated that lipase from Pseudomonas cepacia was very efficient in degrading the nanoparticles. Hydrolysis degradations under basic conditions were also carried out, and a complete degradation was achieved after 4 h. Additionally, cytotoxicity assays were carried out on HEK293 cells, and the results affirmed cell viabilities over 90% when incubated with up to 1 mg/mL nanoparticles for 24 h. These biodegradable and biocompatible nanoparticles hence hold great potential for future applications such as drug release.
Autoren
  • Yiyi Deng
  • Sven Schäfer
  • Devin Kronstein
  • Azra Atabay
  • Moritz Susewind
  • Elisha Krieg
  • Sebastian Seiffert
  • Jens Gaitzsch
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/38039186
DOI
10.1021/acs.biomac.3c00992
eISSN
1526-4602
Ausgabe der Veröffentlichung
1
Zeitschrift
Biomacromolecules
Schlüsselwörter
  • Humans
  • HEK293 Cells
  • Micelles
  • Polymers
  • Hydrolysis
  • Lipase
  • Polyethylene Glycols
Sprache
eng
Country
United States
Paginierung
303 - 314
Datum der Veröffentlichung
2024
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2024
Titel
Amphiphilic Block Copolymers PEG-b-PMTCs: Synthesis, Self-Assembly, Degradation Properties and Biocompatibility.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
25

Datenquelle: PubMed

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