Age- and caste-independent piRNAs in the germline and miRNA profiles linked to caste and fecundity in the ant Temnothorax rugatulus
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Ann-Sophie Seistrup
- Marina Choppin
- Shamitha Govind
- Barbara Feldmeyer
- Marion Kever
- Emil Karaulanov
- Alice Seguret
- Sivarajan Karunanithi
- Miguel V Almeida
- Rene F Ketting
- Susanne Foitzik
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:001086468300001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1111/mec.17162
- eISSN
- 1365-294X
- Externe Identifier
- Clarivate Analytics Document Solution ID: X6VT1
- PubMed Identifier: 37830492
- ISSN
- 0962-1083
- Ausgabe der Veröffentlichung
- 22
- Zeitschrift
- MOLECULAR ECOLOGY
- Schlüsselwörter
- ageing
- castes
- gene regulation
- miRNAs
- social insects
- transposable elements
- Paginierung
- 6027 - 6043
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Titel
- Age- and caste-independent piRNAs in the germline and miRNA profiles linked to caste and fecundity in the ant Temnothorax rugatulus
- Sub types
- Article
- Ausgabe der Zeitschrift
- 32
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Social insects are models for studies of phenotypic plasticity. Ant queens and workers vary in fecundity and lifespan, which are enhanced and extended in queens. Yet, the regulatory mechanisms underlying this variation are not well understood. Ant queens live and reproduce for years, so that they need to protect their germline from transposable element (TE) activity, which may be redundant in short‐lived, often sterile workers. We analysed the expression of two protective classes of small RNAs, microRNAs (miRNAs) and Piwi‐interacting RNAs (piRNAs), in various tissues, castes and age classes of the ant <jats:italic>Temnothorax rugatulus</jats:italic>. In queens, piRNAs were highly abundant in ovaries with TEs being their clear targets, with reduced but still detectable piRNA‐specific ping‐pong signatures in thorax and brains. piRNA pathway activity varied little with age in queens. Moreover, the reduced ovaries of workers also exhibited similar piRNA activity and this not only in young, fertile workers, but also in older foragers with regressed ovaries. Therefore, these ants protect their germline through piRNA activity, regardless of ovarian development, age or caste, even in sterile workers often considered the soma of the <jats:italic>superorganism</jats:italic>. Our tissue‐specific miRNA analysis detected the expression of 304 miRNAs, of which 105 were expressed in all tissues, 10 enriched in the brain, three in the thorax, whereas 83 were ovarian‐specific. We identified ovarian miRNAs whose expression was related to caste, fecundity and age, and which likely regulate group‐specific gene expression. sRNA shifts in young‐ to middle‐aged queens were minor, suggesting delayed senescence in this reproductive caste.</jats:p>
- Autoren
- Ann‐Sophie Seistrup
- Marina Choppin
- Shamitha Govind
- Barbara Feldmeyer
- Marion Kever
- Emil Karaulanov
- Alice Séguret
- Sivarajan Karunanithi
- Miguel V Almeida
- René F Ketting
- Susanne Foitzik
- DOI
- 10.1111/mec.17162
- eISSN
- 1365-294X
- ISSN
- 0962-1083
- Ausgabe der Veröffentlichung
- 22
- Zeitschrift
- Molecular Ecology
- Sprache
- en
- Online publication date
- 2023
- Paginierung
- 6027 - 6043
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1111/mec.17162
- Datum der Datenerfassung
- 2023
- Titel
- Age‐ and caste‐independent <scp>piRNAs</scp> in the germline and <scp>miRNA</scp> profiles linked to caste and fecundity in the ant <i>Temnothorax rugatulus</i>
- Ausgabe der Zeitschrift
- 32
Datenquelle: Crossref
- Abstract
- Social insects are models for studies of phenotypic plasticity. Ant queens and workers vary in fecundity and lifespan, which are enhanced and extended in queens. Yet, the regulatory mechanisms underlying this variation are not well understood. Ant queens live and reproduce for years, so that they need to protect their germline from transposable element (TE) activity, which may be redundant in short-lived, often sterile workers. We analysed the expression of two protective classes of small RNAs, microRNAs (miRNAs) and Piwi-interacting RNAs (piRNAs), in various tissues, castes and age classes of the ant Temnothorax rugatulus. In queens, piRNAs were highly abundant in ovaries with TEs being their clear targets, with reduced but still detectable piRNA-specific ping-pong signatures in thorax and brains. piRNA pathway activity varied little with age in queens. Moreover, the reduced ovaries of workers also exhibited similar piRNA activity and this not only in young, fertile workers, but also in older foragers with regressed ovaries. Therefore, these ants protect their germline through piRNA activity, regardless of ovarian development, age or caste, even in sterile workers often considered the soma of the superorganism. Our tissue-specific miRNA analysis detected the expression of 304 miRNAs, of which 105 were expressed in all tissues, 10 enriched in the brain, three in the thorax, whereas 83 were ovarian-specific. We identified ovarian miRNAs whose expression was related to caste, fecundity and age, and which likely regulate group-specific gene expression. sRNA shifts in young- to middle-aged queens were minor, suggesting delayed senescence in this reproductive caste.
- Addresses
- Institute of Molecular Biology, Mainz, Germany.
- Autoren
- Ann-Sophie Seistrup
- Marina Choppin
- Shamitha Govind
- Barbara Feldmeyer
- Marion Kever
- Emil Karaulanov
- Alice Séguret
- Sivarajan Karunanithi
- Miguel V Almeida
- René F Ketting
- Susanne Foitzik
- DOI
- 10.1111/mec.17162
- eISSN
- 1365-294X
- Externe Identifier
- PubMed Identifier: 37830492
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: GRK2526/1 ‐ Projectnr. 407023052
- Deutsche Forschungsgemeinschaft: GRK2526/1 - Projectnr. 407023052
- Open access
- false
- ISSN
- 0962-1083
- Ausgabe der Veröffentlichung
- 22
- Zeitschrift
- Molecular ecology
- Schlüsselwörter
- Germ Cells
- Animals
- Ants
- MicroRNAs
- Fertility
- Piwi-Interacting RNA
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2023
- Paginierung
- 6027 - 6043
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2023
- Titel
- Age- and caste-independent piRNAs in the germline and miRNA profiles linked to caste and fecundity in the ant Temnothorax rugatulus.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 32
Datenquelle: Europe PubMed Central
- Abstract
- Social insects are models for studies of phenotypic plasticity. Ant queens and workers vary in fecundity and lifespan, which are enhanced and extended in queens. Yet, the regulatory mechanisms underlying this variation are not well understood. Ant queens live and reproduce for years, so that they need to protect their germline from transposable element (TE) activity, which may be redundant in short-lived, often sterile workers. We analysed the expression of two protective classes of small RNAs, microRNAs (miRNAs) and Piwi-interacting RNAs (piRNAs), in various tissues, castes and age classes of the ant Temnothorax rugatulus. In queens, piRNAs were highly abundant in ovaries with TEs being their clear targets, with reduced but still detectable piRNA-specific ping-pong signatures in thorax and brains. piRNA pathway activity varied little with age in queens. Moreover, the reduced ovaries of workers also exhibited similar piRNA activity and this not only in young, fertile workers, but also in older foragers with regressed ovaries. Therefore, these ants protect their germline through piRNA activity, regardless of ovarian development, age or caste, even in sterile workers often considered the soma of the superorganism. Our tissue-specific miRNA analysis detected the expression of 304 miRNAs, of which 105 were expressed in all tissues, 10 enriched in the brain, three in the thorax, whereas 83 were ovarian-specific. We identified ovarian miRNAs whose expression was related to caste, fecundity and age, and which likely regulate group-specific gene expression. sRNA shifts in young- to middle-aged queens were minor, suggesting delayed senescence in this reproductive caste.
- Date of acceptance
- 2023
- Autoren
- Ann-Sophie Seistrup
- Marina Choppin
- Shamitha Govind
- Barbara Feldmeyer
- Marion Kever
- Emil Karaulanov
- Alice Séguret
- Sivarajan Karunanithi
- Miguel V Almeida
- René F Ketting
- Susanne Foitzik
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/37830492
- DOI
- 10.1111/mec.17162
- eISSN
- 1365-294X
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: GRK2526/1 - Projectnr. 407023052
- Ausgabe der Veröffentlichung
- 22
- Zeitschrift
- Mol Ecol
- Schlüsselwörter
- ageing
- castes
- gene regulation
- miRNAs
- social insects
- transposable elements
- Animals
- Piwi-Interacting RNA
- Ants
- Fertility
- MicroRNAs
- Germ Cells
- Sprache
- eng
- Country
- England
- Paginierung
- 6027 - 6043
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2023
- Titel
- Age- and caste-independent piRNAs in the germline and miRNA profiles linked to caste and fecundity in the ant Temnothorax rugatulus.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 32
Datenquelle: PubMed
- Author's licence
- CC-BY
- Autoren
- Ann-Sophie Seistrup
- Marina Choppin
- Shamitha Govind
- Barbara Feldmeyer
- Marion Kever
- Emil Karaulanov
- Alice Séguret
- Sivarajan Karunanithi
- Miguel V Almeida
- René F Ketting
- Susanne Foitzik
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- DFG-491381577-H
- Resource version
- Published version
- DOI
- 10.1111/mec.17162
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 1365-294X
- Ausgabe der Veröffentlichung
- 22
- Zeitschrift
- Molecular ecology
- Schlüsselwörter
- 570 Biowissenschaften
- 570 Life sciences
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- 6027 - 6043
- Datum der Veröffentlichung
- 2023
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/9781
- Herausgeber
- Wiley-Blackwell
- Datum der Datenerfassung
- 2023
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2023
- Zugang
- Public
- Titel
- Age- and caste-independent piRNAs in the germline and miRNA profiles linked to caste and fecundity in the ant Temnothorax rugatulus
- Ausgabe der Zeitschrift
- 32
Files
age_and_casteindependent_pirn-20231206115117244.pdf
Datenquelle: OPENSCIENCE.UB
- Beziehungen:
- Eigentum von