Insights into functional aspects of centrins from the structure of N-terminally extended mouse centrin 1

Autoren

Jung Hee Park
Alexander Pulvermüller
Patrick Scheerer
Sebastian Rausch
Andreas Gießl
Wolfgang Höhne
Uwe Wolfrum
Klaus Peter Hofmann
Oliver Peter Ernst
Hui-Woog Choe
Norbert Krauß

DOI

10.1016/j.visres.2006.07.034

ISSN

0042-6989

Ausgabe der Veröffentlichung

27

Zeitschrift

Vision Research

Sprache

en

Paginierung

4568 - 4574

Datum der Veröffentlichung

2006

Status

Published

Herausgeber

Elsevier BV

Herausgeber URL

http://dx.doi.org/10.1016/j.visres.2006.07.034

Datum der Datenerfassung

2021

Titel

Insights into functional aspects of centrins from the structure of N-terminally extended mouse centrin 1

Ausgabe der Zeitschrift

46

Datenquelle: Crossref

Abstract

Centrins are members of the family of Ca(2+)-binding EF-hand proteins. In photoreceptor cells, centrin isoform 1 is specifically localized in the non-motile cilium. This connecting cilium links the light-sensitive outer segment with the biosynthetic active inner segment of the photoreceptor cell. All intracellular exchanges between these compartments have to occur through this cilium. Three-dimensional structures of centrins from diverse organisms are known, showing that the EF-hand motifs of the N-terminal domains adopt closed conformations, while the C-terminal EF-hand motifs have open conformations. The crystal structure of an N-terminally extended mouse centrin 1 (MmCen1-L) resembles the overall structure of troponin C in its two Ca(2+) bound form. Within the N-terminal extension in MmCen1-L, residues W24 and R25 bind to the C-terminal domain of centrin 1 in a target-protein-like geometry. Here, we discuss this binding mode in connection with putative interaction sites of the target-protein transducin and the self-assembly of centrins.

Addresses

Institut für Medizinische Physik und Biophysik, Charité-Universitätsmedizin Berlin, Ziegelstr. 5-9, D-10098 Berlin, Germany.

Autoren

Jung Hee Park
Alexander Pulvermüller
Patrick Scheerer
Sebastian Rausch
Andreas Giessl
Wolfgang Höhne
Uwe Wolfrum
Klaus Peter Hofmann
Oliver Peter Ernst
Hui-Woog Choe
Norbert Krauss

DOI

10.1016/j.visres.2006.07.034

eISSN

1878-5646

Externe Identifier

PubMed Identifier: 17027898

Open access

false

ISSN

0042-6989

Ausgabe der Veröffentlichung

27

Zeitschrift

Vision research

Schlüsselwörter

Cilia
Animals
Humans
Mice
Calcium
Calcium-Binding Proteins
Amino Acid Motifs
Terminal Repeat Sequences
Protein Conformation
Protein Structure, Quaternary
EF Hand Motifs
Protein Structure, Tertiary
Protein Binding
Sequence Homology
Structure-Activity Relationship
Photoreceptor Cells

Sprache

eng

Medium

Print-Electronic

Online publication date

2006

Paginierung

4568 - 4574

Datum der Veröffentlichung

2006

Status

Published

Datum der Datenerfassung

2006

Titel

Insights into functional aspects of centrins from the structure of N-terminally extended mouse centrin 1.

Sub types

Research Support, Non-U.S. Gov't
Review
Journal Article

Ausgabe der Zeitschrift

46

Datenquelle: Europe PubMed Central

Abstract

Centrins are members of the family of Ca(2+)-binding EF-hand proteins. In photoreceptor cells, centrin isoform 1 is specifically localized in the non-motile cilium. This connecting cilium links the light-sensitive outer segment with the biosynthetic active inner segment of the photoreceptor cell. All intracellular exchanges between these compartments have to occur through this cilium. Three-dimensional structures of centrins from diverse organisms are known, showing that the EF-hand motifs of the N-terminal domains adopt closed conformations, while the C-terminal EF-hand motifs have open conformations. The crystal structure of an N-terminally extended mouse centrin 1 (MmCen1-L) resembles the overall structure of troponin C in its two Ca(2+) bound form. Within the N-terminal extension in MmCen1-L, residues W24 and R25 bind to the C-terminal domain of centrin 1 in a target-protein-like geometry. Here, we discuss this binding mode in connection with putative interaction sites of the target-protein transducin and the self-assembly of centrins.

Date of acceptance

2006

Autoren

Jung Hee Park
Alexander Pulvermüller
Patrick Scheerer
Sebastian Rausch
Andreas Giessl
Wolfgang Höhne
Uwe Wolfrum
Klaus Peter Hofmann
Oliver Peter Ernst
Hui-Woog Choe
Norbert Krauss

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/17027898

DOI

10.1016/j.visres.2006.07.034

ISSN

0042-6989

Ausgabe der Veröffentlichung

27

Zeitschrift

Vision Res

Schlüsselwörter

Amino Acid Motifs
Animals
Calcium
Calcium-Binding Proteins
Cilia
EF Hand Motifs
Humans
Mice
Photoreceptor Cells
Protein Binding
Protein Conformation
Protein Structure, Quaternary
Protein Structure, Tertiary
Sequence Homology
Structure-Activity Relationship
Terminal Repeat Sequences

Sprache

eng

Country

England

Paginierung

4568 - 4574

PII

S0042-6989(06)00362-2

Datum der Veröffentlichung

2006

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2007

Titel

Insights into functional aspects of centrins from the structure of N-terminally extended mouse centrin 1.

Sub types

Journal Article
Research Support, Non-U.S. Gov't
Review

Ausgabe der Zeitschrift

46

Datenquelle: PubMed

Insights into functional aspects of centrins from the structure of N-terminally extended mouse centrin 1

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