Effects of Presynaptic Mutations on a Postsynaptic Cacna1s Calcium Channel Colocalized with mGluR6 at Mouse Photoreceptor Ribbon Synapses
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Dana Specht
- Shu-Biao Wu
- Paul Turner
- Peter Dearden
- Frank Koentgen
- Uwe Wolfrum
- Marion Maw
- Johann Helmut Brandstaetter
- Susanne tom Dieck
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000262665900003&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1167/iovs.08-2758
- eISSN
- 1552-5783
- Externe Identifier
- Clarivate Analytics Document Solution ID: 397MD
- PubMed Identifier: 18952919
- ISSN
- 0146-0404
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
- Paginierung
- 505 - 515
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Titel
- Effects of Presynaptic Mutations on a Postsynaptic Cacna1s Calcium Channel Colocalized with mGluR6 at Mouse Photoreceptor Ribbon Synapses
- Sub types
- Article
- Ausgabe der Zeitschrift
- 50
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Dana Specht
- Shu-Biao Wu
- Paul Turner
- Peter Dearden
- Frank Koentgen
- Uwe Wolfrum
- Marion Maw
- Johann Helmut Brandsta¨tter
- Susanne tom Dieck
- DOI
- 10.1167/iovs.08-2758
- ISSN
- 1552-5783
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Investigative Opthalmology & Visual Science
- Sprache
- en
- Paginierung
- 505 - 505
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Herausgeber
- Association for Research in Vision and Ophthalmology (ARVO)
- Herausgeber URL
- http://dx.doi.org/10.1167/iovs.08-2758
- Datum der Datenerfassung
- 2017
- Titel
- Effects of Presynaptic Mutations on a Postsynaptic Cacna1s Calcium Channel Colocalized with mGluR6 at Mouse Photoreceptor Ribbon Synapses
- Ausgabe der Zeitschrift
- 50
Datenquelle: Crossref
- Abstract
- <h4>Purpose</h4>Photoreceptor ribbon synapses translate light-dependent changes of membrane potential into graded transmitter release via L-type voltage-dependent calcium channel (VDCC) activity. Functional abnormalities (e.g., a reduced electroretinogram b-wave), arising from mutations of presynaptic proteins, such as Bassoon and the VDCCalpha1 subunit Cacna1f, have been shown to altered transmitter release. L-type VDCCalpha1 subtype expression in wild-type and mutant mice was examined, to investigate the underlying pathologic mechanism.<h4>Methods</h4>Two antisera against Cacna1f, and a Cacna1f mouse mutant (Cacna1fDeltaEx14-17) were generated. Immunocytochemistry for L-type VDCCalpha1 subunits and additional synaptic marker proteins was performed in wild-type, BassoonDeltaEx4-5 and Cacna1fDeltaEx14-17 mice.<h4>Results</h4>Active zone staining at photoreceptor ribbon synapses with a panalpha1 antibody colocalized with staining for Cacna1f in wild-type mouse retina. Similarly, in the BassoonDeltaEx4-5 mouse, residual mislocalized staining for panalpha1 and Cacna1f showed colocalization. Unlike the presynaptic location of Cacna1f and panalpha1 antibody staining, the skeletal muscle VDCCalpha1 subunit Cacna1s was present postsynaptically at ON-bipolar cell dendrites, where it colocalized with metabotropic glutamate receptor 6 (mGluR6). Surprisingly, Cacna1s labeling was severely downregulated in the BassoonDeltaEx4-5 and Cacna1fDeltaEx14-17 mutants. Subsequent analyses revealed severely reduced ON-bipolar cell dendritic expression of the sarcoplasmic reticulum Ca(2+) ATPase Serca2 in both mouse mutants and of mGluR6 in the Cacna1fDeltaEx14-17 mutant.<h4>Conclusions</h4>Presynaptic mutations leading to reduced photoreceptor-to-bipolar cell signaling are associated with disturbances in protein expression within postsynaptic dendrites. Moreover, detection of Cacna1s and Serca2 in ON-bipolar cell dendrites in wild-type animals suggests a putative role in regulation of postsynaptic Ca(2+) flux.
- Addresses
- Department of Biology, Animal Physiology, University of Erlangen-Nuremberg, Erlangen, Germany.
- Autoren
- Dana Specht
- Shu-Biao Wu
- Paul Turner
- Peter Dearden
- Frank Koentgen
- Uwe Wolfrum
- Marion Maw
- Johann Helmut Brandstätter
- Susanne tom Dieck
- DOI
- 10.1167/iovs.08-2758
- eISSN
- 1552-5783
- Externe Identifier
- PubMed Identifier: 18952919
- Open access
- false
- ISSN
- 0146-0404
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Investigative ophthalmology & visual science
- Schlüsselwörter
- Presynaptic Terminals
- Animals
- Mice, Inbred C57BL
- Sheep
- Rabbits
- Mice
- Mice, Mutant Strains
- Peptide Fragments
- Calcium Channels
- Calcium Channels, L-Type
- Receptors, Metabotropic Glutamate
- Epitopes
- Microscopy, Immunoelectron
- Fluorescent Antibody Technique, Indirect
- Blotting, Western
- Blotting, Northern
- Reverse Transcriptase Polymerase Chain Reaction
- Calcium Signaling
- Synaptic Transmission
- Sequence Deletion
- Female
- Male
- Photoreceptor Cells, Vertebrate
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2008
- Paginierung
- 505 - 515
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum der Datenerfassung
- 2008
- Titel
- Effects of presynaptic mutations on a postsynaptic Cacna1s calcium channel colocalized with mGluR6 at mouse photoreceptor ribbon synapses.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 50
Datenquelle: Europe PubMed Central
- Abstract
- PURPOSE: Photoreceptor ribbon synapses translate light-dependent changes of membrane potential into graded transmitter release via L-type voltage-dependent calcium channel (VDCC) activity. Functional abnormalities (e.g., a reduced electroretinogram b-wave), arising from mutations of presynaptic proteins, such as Bassoon and the VDCCalpha1 subunit Cacna1f, have been shown to altered transmitter release. L-type VDCCalpha1 subtype expression in wild-type and mutant mice was examined, to investigate the underlying pathologic mechanism. METHODS: Two antisera against Cacna1f, and a Cacna1f mouse mutant (Cacna1fDeltaEx14-17) were generated. Immunocytochemistry for L-type VDCCalpha1 subunits and additional synaptic marker proteins was performed in wild-type, BassoonDeltaEx4-5 and Cacna1fDeltaEx14-17 mice. RESULTS: Active zone staining at photoreceptor ribbon synapses with a panalpha1 antibody colocalized with staining for Cacna1f in wild-type mouse retina. Similarly, in the BassoonDeltaEx4-5 mouse, residual mislocalized staining for panalpha1 and Cacna1f showed colocalization. Unlike the presynaptic location of Cacna1f and panalpha1 antibody staining, the skeletal muscle VDCCalpha1 subunit Cacna1s was present postsynaptically at ON-bipolar cell dendrites, where it colocalized with metabotropic glutamate receptor 6 (mGluR6). Surprisingly, Cacna1s labeling was severely downregulated in the BassoonDeltaEx4-5 and Cacna1fDeltaEx14-17 mutants. Subsequent analyses revealed severely reduced ON-bipolar cell dendritic expression of the sarcoplasmic reticulum Ca(2+) ATPase Serca2 in both mouse mutants and of mGluR6 in the Cacna1fDeltaEx14-17 mutant. CONCLUSIONS: Presynaptic mutations leading to reduced photoreceptor-to-bipolar cell signaling are associated with disturbances in protein expression within postsynaptic dendrites. Moreover, detection of Cacna1s and Serca2 in ON-bipolar cell dendrites in wild-type animals suggests a putative role in regulation of postsynaptic Ca(2+) flux.
- Autoren
- Dana Specht
- Shu-Biao Wu
- Paul Turner
- Peter Dearden
- Frank Koentgen
- Uwe Wolfrum
- Marion Maw
- Johann Helmut Brandstätter
- Susanne tom Dieck
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/18952919
- DOI
- 10.1167/iovs.08-2758
- eISSN
- 1552-5783
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Invest Ophthalmol Vis Sci
- Schlüsselwörter
- Animals
- Blotting, Northern
- Blotting, Western
- Calcium Channels
- Calcium Channels, L-Type
- Calcium Signaling
- Epitopes
- Female
- Fluorescent Antibody Technique, Indirect
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Mutant Strains
- Microscopy, Immunoelectron
- Peptide Fragments
- Photoreceptor Cells, Vertebrate
- Presynaptic Terminals
- Rabbits
- Receptors, Metabotropic Glutamate
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Deletion
- Sheep
- Synaptic Transmission
- Sprache
- eng
- Country
- United States
- Paginierung
- 505 - 515
- PII
- iovs.08-2758
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2009
- Titel
- Effects of presynaptic mutations on a postsynaptic Cacna1s calcium channel colocalized with mGluR6 at mouse photoreceptor ribbon synapses.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 50
Datenquelle: PubMed
- Beziehungen:
- Eigentum von