Refinement of strategies for the development of a human cytomegalovirus dense body vaccine
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Veronique Mersseman
- Verena Boehm
- Rafaela Holtappels
- Petra Deegen
- Uwe Wolfrum
- Bodo Plachter
- Sabine Reyda
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000255033900004&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1007/s00430-008-0085-2
- eISSN
- 1432-1831
- Externe Identifier
- Clarivate Analytics Document Solution ID: 289CZ
- PubMed Identifier: 18320219
- ISSN
- 0300-8584
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- MEDICAL MICROBIOLOGY AND IMMUNOLOGY
- Schlüsselwörter
- cytomegalovirus
- vaccine
- dense bodies
- pp65
- IE1
- Paginierung
- 97 - 107
- Datum der Veröffentlichung
- 2008
- Status
- Published
- Titel
- Refinement of strategies for the development of a human cytomegalovirus dense body vaccine
- Sub types
- Article
- Ausgabe der Zeitschrift
- 197
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Véronique Mersseman
- Verena Böhm
- Rafaela Holtappels
- Petra Deegen
- Uwe Wolfrum
- Bodo Plachter
- Sabine Reyda
- DOI
- 10.1007/s00430-008-0085-2
- eISSN
- 1432-1831
- ISSN
- 0300-8584
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Medical Microbiology and Immunology
- Sprache
- en
- Online publication date
- 2008
- Paginierung
- 97 - 107
- Datum der Veröffentlichung
- 2008
- Status
- Published
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1007/s00430-008-0085-2
- Datum der Datenerfassung
- 2023
- Titel
- Refinement of strategies for the development of a human cytomegalovirus dense body vaccine
- Ausgabe der Zeitschrift
- 197
Datenquelle: Crossref
- Abstract
- Development of a vaccine against human cytomegalovirus (HCMV) infection has been identified as a high priority goal in biomedical research, yet no vaccine has been licensed until now. Recombinant subviral dense bodies (recDB) are a promising basis for the establishment of such a vaccine. In this article, strategies for the generation of recDB, based on recombination-mediated genetic engineering of the 230 kb HCMV DNA genome in E. coli are outlined. Analysis of viral mutants that were constructed in this process provided the proof-of-principle that heterologous antigens can be packaged into recDB and that these particles prime CD8 T cell responses against the recombinant antigen upon their application to HLA-A2 transgenic mice.
- Addresses
- Institute for Virology, Johannes Gutenberg-Universität, Obere Zahlbacher Str. 67, 55101 Mainz, Germany.
- Autoren
- Véronique Mersseman
- Verena Böhm
- Rafaela Holtappels
- Petra Deegen
- Uwe Wolfrum
- Bodo Plachter
- Sabine Reyda
- DOI
- 10.1007/s00430-008-0085-2
- eISSN
- 1432-1831
- Externe Identifier
- PubMed Identifier: 18320219
- Open access
- false
- ISSN
- 0300-8584
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Medical microbiology and immunology
- Schlüsselwörter
- T-Lymphocytes
- Animals
- Mice, Transgenic
- Mice
- Escherichia coli
- Cytomegalovirus
- Cytomegalovirus Infections
- Recombinant Proteins
- Vaccines, Synthetic
- DNA, Viral
- Cytomegalovirus Vaccines
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2008
- Paginierung
- 97 - 107
- Datum der Veröffentlichung
- 2008
- Status
- Published
- Datum der Datenerfassung
- 2008
- Titel
- Refinement of strategies for the development of a human cytomegalovirus dense body vaccine.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 197
Datenquelle: Europe PubMed Central
- Abstract
- Development of a vaccine against human cytomegalovirus (HCMV) infection has been identified as a high priority goal in biomedical research, yet no vaccine has been licensed until now. Recombinant subviral dense bodies (recDB) are a promising basis for the establishment of such a vaccine. In this article, strategies for the generation of recDB, based on recombination-mediated genetic engineering of the 230 kb HCMV DNA genome in E. coli are outlined. Analysis of viral mutants that were constructed in this process provided the proof-of-principle that heterologous antigens can be packaged into recDB and that these particles prime CD8 T cell responses against the recombinant antigen upon their application to HLA-A2 transgenic mice.
- Autoren
- Véronique Mersseman
- Verena Böhm
- Rafaela Holtappels
- Petra Deegen
- Uwe Wolfrum
- Bodo Plachter
- Sabine Reyda
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/18320219
- DOI
- 10.1007/s00430-008-0085-2
- ISSN
- 0300-8584
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Med Microbiol Immunol
- Schlüsselwörter
- Animals
- Cytomegalovirus
- Cytomegalovirus Infections
- Cytomegalovirus Vaccines
- DNA, Viral
- Escherichia coli
- Mice
- Mice, Transgenic
- Recombinant Proteins
- T-Lymphocytes
- Vaccines, Synthetic
- Sprache
- eng
- Country
- Germany
- Paginierung
- 97 - 107
- Datum der Veröffentlichung
- 2008
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2008
- Titel
- Refinement of strategies for the development of a human cytomegalovirus dense body vaccine.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 197
Datenquelle: PubMed
- Beziehungen:
- Eigentum von