Optimized recombinant dense bodies of human cytomegalovirus efficiently prime virus specific lymphocytes and neutralizing antibodies without the addition of adjuvant
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Sabine Becke
- Steffi Aue
- Doris Thomas
- Sina Schader
- Juergen Podlech
- Tobias Bopp
- Tina Sedmak
- Uwe Wolfrum
- Bodo Plachter
- Sabine Reyda
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000282150700012&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.vaccine.2010.07.016
- eISSN
- 1873-2518
- Externe Identifier
- Clarivate Analytics Document Solution ID: 654EG
- PubMed Identifier: 20655401
- ISSN
- 0264-410X
- Ausgabe der Veröffentlichung
- 38
- Zeitschrift
- VACCINE
- Schlüsselwörter
- Cytomegalovirus
- Dense bodies
- CD8 T cells
- Paginierung
- 6191 - 6198
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Titel
- Optimized recombinant dense bodies of human cytomegalovirus efficiently prime virus specific lymphocytes and neutralizing antibodies without the addition of adjuvant
- Sub types
- Article
- Ausgabe der Zeitschrift
- 28
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Sabine Becke
- Steffi Aue
- Doris Thomas
- Sina Schader
- Jürgen Podlech
- Tobias Bopp
- Tina Sedmak
- Uwe Wolfrum
- Bodo Plachter
- Sabine Reyda
- DOI
- 10.1016/j.vaccine.2010.07.016
- ISSN
- 0264-410X
- Ausgabe der Veröffentlichung
- 38
- Zeitschrift
- Vaccine
- Sprache
- en
- Paginierung
- 6191 - 6198
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.vaccine.2010.07.016
- Datum der Datenerfassung
- 2020
- Titel
- Optimized recombinant dense bodies of human cytomegalovirus efficiently prime virus specific lymphocytes and neutralizing antibodies without the addition of adjuvant
- Ausgabe der Zeitschrift
- 28
Datenquelle: Crossref
- Abstract
- Control of human cytomegalovirus (HCMV) infection correlates with the reconstitution of antiviral T lymphocytes in haematopoietic stem cell transplant recipients. A vaccine to foster this reconstitution and to ameliorate the severe consequences of HCMV reactivation is yet unavailable. This work focused on providing a rationale for the amendment of the yields and the antigenic composition of a vaccine, based on subviral dense bodies (DB) of HCMV. Modified DB were generated that contained the HLA-A2 presented IE1 model peptide TMYGGISLL, integrated at different positions in the major DB protein pp65. Insertion at position W175 of pp65 allowed efficient formation of recDB in the cytoplasm of infected cells and resulted in considerable yields of these particles. Even in the absence of adjuvant, these particles proved to be highly immunogenic with respect to CD8 and CD4 T cell and neutralizing antibody responses.
- Addresses
- Institute for Virology, University Medical Centre, Johannes Gutenberg-University Mainz, Obere Zahlbacher Str. 67, D-55101 Mainz, Germany.
- Autoren
- Sabine Becke
- Steffi Aue
- Doris Thomas
- Sina Schader
- Jürgen Podlech
- Tobias Bopp
- Tina Sedmak
- Uwe Wolfrum
- Bodo Plachter
- Sabine Reyda
- DOI
- 10.1016/j.vaccine.2010.07.016
- eISSN
- 1873-2518
- Externe Identifier
- PubMed Identifier: 20655401
- Open access
- false
- ISSN
- 0264-410X
- Ausgabe der Veröffentlichung
- 38
- Zeitschrift
- Vaccine
- Schlüsselwörter
- CD4-Positive T-Lymphocytes
- CD8-Positive T-Lymphocytes
- Cell Line
- Animals
- Mice, Inbred BALB C
- Mice, Transgenic
- Humans
- Mice
- Cytomegalovirus
- Cytomegalovirus Infections
- Phosphoproteins
- Viral Matrix Proteins
- DNA, Viral
- Cytomegalovirus Vaccines
- Antibodies, Viral
- Antigens, Viral
- Mutagenesis, Insertional
- Antibodies, Neutralizing
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2010
- Paginierung
- 6191 - 6198
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Datum der Datenerfassung
- 2010
- Titel
- Optimized recombinant dense bodies of human cytomegalovirus efficiently prime virus specific lymphocytes and neutralizing antibodies without the addition of adjuvant.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 28
Datenquelle: Europe PubMed Central
- Abstract
- Control of human cytomegalovirus (HCMV) infection correlates with the reconstitution of antiviral T lymphocytes in haematopoietic stem cell transplant recipients. A vaccine to foster this reconstitution and to ameliorate the severe consequences of HCMV reactivation is yet unavailable. This work focused on providing a rationale for the amendment of the yields and the antigenic composition of a vaccine, based on subviral dense bodies (DB) of HCMV. Modified DB were generated that contained the HLA-A2 presented IE1 model peptide TMYGGISLL, integrated at different positions in the major DB protein pp65. Insertion at position W175 of pp65 allowed efficient formation of recDB in the cytoplasm of infected cells and resulted in considerable yields of these particles. Even in the absence of adjuvant, these particles proved to be highly immunogenic with respect to CD8 and CD4 T cell and neutralizing antibody responses.
- Date of acceptance
- 2010
- Autoren
- Sabine Becke
- Steffi Aue
- Doris Thomas
- Sina Schader
- Jürgen Podlech
- Tobias Bopp
- Tina Sedmak
- Uwe Wolfrum
- Bodo Plachter
- Sabine Reyda
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/20655401
- DOI
- 10.1016/j.vaccine.2010.07.016
- eISSN
- 1873-2518
- Ausgabe der Veröffentlichung
- 38
- Zeitschrift
- Vaccine
- Schlüsselwörter
- Animals
- Antibodies, Neutralizing
- Antibodies, Viral
- Antigens, Viral
- CD4-Positive T-Lymphocytes
- CD8-Positive T-Lymphocytes
- Cell Line
- Cytomegalovirus
- Cytomegalovirus Infections
- Cytomegalovirus Vaccines
- DNA, Viral
- Humans
- Mice
- Mice, Inbred BALB C
- Mice, Transgenic
- Mutagenesis, Insertional
- Phosphoproteins
- Viral Matrix Proteins
- Sprache
- eng
- Country
- Netherlands
- Paginierung
- 6191 - 6198
- PII
- S0264-410X(10)00999-0
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2010
- Titel
- Optimized recombinant dense bodies of human cytomegalovirus efficiently prime virus specific lymphocytes and neutralizing antibodies without the addition of adjuvant.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 28
Datenquelle: PubMed
- Beziehungen:
- Eigentum von