Septins 2, 7 and 9 and MAP4 colocalize along the axoneme in the primary cilium and control ciliary length
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Rania Ghossoub
- Qicong Hu
- Marion Failler
- Marie-Christine Rouyez
- Benjamin Spitzbarth
- Serge Mostowy
- Uwe Wolfrum
- Sophie Saunier
- Pascale Cossart
- W James Nelson
- Alexandre Benmerah
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000320705300006&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1242/jcs.111377
- eISSN
- 1477-9137
- Externe Identifier
- Clarivate Analytics Document Solution ID: 168KT
- PubMed Identifier: 23572511
- ISSN
- 0021-9533
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- JOURNAL OF CELL SCIENCE
- Schlüsselwörter
- Primary cilium
- Septins
- MAP4
- Microtubules
- Actin
- Paginierung
- 2583 - 2594
- Datum der Veröffentlichung
- 2013
- Status
- Published
- Titel
- Septins 2, 7 and 9 and MAP4 colocalize along the axoneme in the primary cilium and control ciliary length
- Sub types
- Article
- Ausgabe der Zeitschrift
- 126
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:p>Septins are a large, evolutionarily conserved family of GTPases that form hetero-oligomers and interact with the actin-based cytoskeleton and microtubules. They are involved in scaffolding functions, and form diffusion barriers in budding yeast, the sperm flagellum and the base of primary cilia of kidney epithelial cells. We investigated the role of septins in the primary cilium of retinal pigmented epithelial (RPE) cells, and found that SEPT2 forms a 1∶1∶1 complex with SEPT7 and SEPT9 and that the three members of this complex co-localize along the length of the axoneme. Similar to observations in kidney epithelial cells, depletion of cilium-localized septins by siRNA-based approaches inhibited ciliogenesis. MAP4, which is a binding partner of SEPT2 and controls the accessibility of septins to microtubules, was also localized to the axoneme where it appeared to negatively regulate ciliary length. Taken together, our data provide new insights into the functions and regulation of septins and MAP4 in the organization of the primary cilium and microtubule-based activities in cells.</jats:p>
- Autoren
- Rania Ghossoub
- Qicong Hu
- Marion Failler
- Marie-Christine Rouyez
- Benjamin Spitzbarth
- Serge Mostowy
- Uwe Wolfrum
- Sophie Saunier
- Pascale Cossart
- W James Nelson
- Alexandre Benmerah
- DOI
- 10.1242/jcs.111377
- eISSN
- 1477-9137
- ISSN
- 0021-9533
- Zeitschrift
- Journal of Cell Science
- Sprache
- en
- Online publication date
- 2013
- Status
- Published online
- Herausgeber
- The Company of Biologists
- Herausgeber URL
- http://dx.doi.org/10.1242/jcs.111377
- Datum der Datenerfassung
- 2024
- Titel
- Septins 2, 7, and 9 and MAP4 co-localize along the axoneme in the primary cilium and control ciliary length
Datenquelle: Crossref
- Abstract
- Septins are a large, evolutionarily conserved family of GTPases that form hetero-oligomers and interact with the actin-based cytoskeleton and microtubules. They are involved in scaffolding functions, and form diffusion barriers in budding yeast, the sperm flagellum and the base of primary cilia of kidney epithelial cells. We investigated the role of septins in the primary cilium of retinal pigmented epithelial (RPE) cells, and found that SEPT2 forms a 1:1:1 complex with SEPT7 and SEPT9 and that the three members of this complex colocalize along the length of the axoneme. Similar to observations in kidney epithelial cells, depletion of cilium-localized septins by siRNA-based approaches inhibited ciliogenesis. MAP4, which is a binding partner of SEPT2 and controls the accessibility of septins to microtubules, was also localized to the axoneme where it appeared to negatively regulate ciliary length. Taken together, our data provide new insights into the functions and regulation of septins and MAP4 in the organization of the primary cilium and microtubule-based activities in cells.
- Addresses
- INSERM U1016, Insitut Cochin, Paris, France.
- Autoren
- Rania Ghossoub
- Qicong Hu
- Marion Failler
- Marie-Christine Rouyez
- Benjamin Spitzbarth
- Serge Mostowy
- Uwe Wolfrum
- Sophie Saunier
- Pascale Cossart
- W Jamesnelson
- Alexandre Benmerah
- DOI
- 10.1242/jcs.111377
- eISSN
- 1477-9137
- Externe Identifier
- PubMed Identifier: 23572511
- PubMed Central ID: PMC3687695
- Funding acknowledgements
- NIGMS NIH HHS: R01 GM035527
- European Research Council: 233348
- NIGMS NIH HHS: GM35527
- NIGMS NIH HHS: R37 GM035527
- Wellcome Trust: WT097411MA
- Open access
- false
- ISSN
- 0021-9533
- Ausgabe der Veröffentlichung
- Pt 12
- Zeitschrift
- Journal of cell science
- Schlüsselwörter
- Retina
- Cell Line
- Cilia
- Epithelial Cells
- Humans
- Actins
- Cell Cycle Proteins
- Microtubule-Associated Proteins
- Axoneme
- Septins
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2013
- Paginierung
- 2583 - 2594
- Datum der Veröffentlichung
- 2013
- Status
- Published
- Datum der Datenerfassung
- 2013
- Titel
- Septins 2, 7 and 9 and MAP4 colocalize along the axoneme in the primary cilium and control ciliary length.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Research Support, U.S. Gov't, Non-P.H.S.
- Journal Article
- Research Support, N.I.H., Extramural
- Ausgabe der Zeitschrift
- 126
Datenquelle: Europe PubMed Central
- Abstract
- Septins are a large, evolutionarily conserved family of GTPases that form hetero-oligomers and interact with the actin-based cytoskeleton and microtubules. They are involved in scaffolding functions, and form diffusion barriers in budding yeast, the sperm flagellum and the base of primary cilia of kidney epithelial cells. We investigated the role of septins in the primary cilium of retinal pigmented epithelial (RPE) cells, and found that SEPT2 forms a 1:1:1 complex with SEPT7 and SEPT9 and that the three members of this complex colocalize along the length of the axoneme. Similar to observations in kidney epithelial cells, depletion of cilium-localized septins by siRNA-based approaches inhibited ciliogenesis. MAP4, which is a binding partner of SEPT2 and controls the accessibility of septins to microtubules, was also localized to the axoneme where it appeared to negatively regulate ciliary length. Taken together, our data provide new insights into the functions and regulation of septins and MAP4 in the organization of the primary cilium and microtubule-based activities in cells.
- Autoren
- Rania Ghossoub
- Qicong Hu
- Marion Failler
- Marie-Christine Rouyez
- Benjamin Spitzbarth
- Serge Mostowy
- Uwe Wolfrum
- Sophie Saunier
- Pascale Cossart
- W Jamesnelson
- Alexandre Benmerah
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/23572511
- DOI
- 10.1242/jcs.111377
- eISSN
- 1477-9137
- Externe Identifier
- PubMed Central ID: PMC3687695
- Funding acknowledgements
- European Research Council: 233348
- NIGMS NIH HHS: GM35527
- NIGMS NIH HHS: R01 GM035527
- Wellcome Trust: WT097411MA
- NIGMS NIH HHS: R37 GM035527
- Ausgabe der Veröffentlichung
- Pt 12
- Zeitschrift
- J Cell Sci
- Schlüsselwörter
- Actin
- MAP4
- Microtubules
- Primary cilium
- Septins
- Actins
- Axoneme
- Cell Cycle Proteins
- Cell Line
- Cilia
- Epithelial Cells
- Humans
- Microtubule-Associated Proteins
- Retina
- Septins
- Sprache
- eng
- Country
- England
- Paginierung
- 2583 - 2594
- PII
- jcs.111377
- Datum der Veröffentlichung
- 2013
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2014
- Titel
- Septins 2, 7 and 9 and MAP4 colocalize along the axoneme in the primary cilium and control ciliary length.
- Sub types
- Journal Article
- Research Support, N.I.H., Extramural
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, Non-P.H.S.
- Ausgabe der Zeitschrift
- 126
Datenquelle: PubMed
- Beziehungen:
- Eigentum von