The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Helder C Ferreira
  • Brian Luke
  • Heiko Schober
  • Veronique Kalck
  • Joachim Lingner
  • Susan M Gasser
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000292305700018&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1038/ncb2263
eISSN
1476-4679
Externe Identifier
  • Clarivate Analytics Document Solution ID: 786KD
  • PubMed Identifier: 21666682
ISSN
1465-7392
Ausgabe der Veröffentlichung
7
Zeitschrift
NATURE CELL BIOLOGY
Paginierung
867 - U313
Datum der Veröffentlichung
2011
Status
Published
Titel
The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast
Sub types
  • Article
Ausgabe der Zeitschrift
13

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Autoren
  • Helder C Ferreira
  • Brian Luke
  • Heiko Schober
  • Véronique Kalck
  • Joachim Lingner
  • Susan M Gasser
DOI
10.1038/ncb2263
eISSN
1476-4679
ISSN
1465-7392
Ausgabe der Veröffentlichung
7
Zeitschrift
Nature Cell Biology
Sprache
en
Online publication date
2011
Paginierung
867 - 874
Datum der Veröffentlichung
2011
Status
Published
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1038/ncb2263
Datum der Datenerfassung
2023
Titel
The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast
Ausgabe der Zeitschrift
13

Datenquelle: Crossref

Abstract
Budding yeast telomeres are reversibly bound at the nuclear envelope through two partially redundant pathways that involve the Sir2/3/4 silencing complex and the Yku70/80 heterodimer. To better understand how this is regulated, we studied the role of SUMOylation in telomere anchoring. We find that the PIAS-like SUMO E3 ligase Siz2 sumoylates both Yku70/80 and Sir4 in vivo and promotes telomere anchoring to the nuclear envelope. Remarkably, loss of Siz2 also provokes telomere extension in a telomerase-dependent manner that is epistatic with loss of the helicase Pif1. Consistent with our previously documented role for telomerase in anchorage, normal telomere anchoring in siz2 Δ is restored by PIF1 deletion. By live-cell imaging of a critically short telomere, we show that telomeres shift away from the nuclear envelope when elongating. We propose that SUMO-dependent association with the nuclear periphery restrains bound telomerase, whereas active elongation correlates with telomere release.
Addresses
  • Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.
Autoren
  • Helder C Ferreira
  • Brian Luke
  • Heiko Schober
  • Véronique Kalck
  • Joachim Lingner
  • Susan M Gasser
DOI
10.1038/ncb2263
eISSN
1476-4679
Externe Identifier
  • PubMed Identifier: 21666682
Funding acknowledgements
  • European Research Council: 232812
  • European Commission FP7: FP7_200950
  • European Commission FP7: FP7_232812
Open access
false
ISSN
1465-7392
Ausgabe der Veröffentlichung
7
Zeitschrift
Nature cell biology
Schlüsselwörter
  • Nuclear Envelope
  • Telomere
  • Saccharomyces cerevisiae
  • DNA Helicases
  • Telomerase
  • DNA-Binding Proteins
  • Saccharomyces cerevisiae Proteins
  • Silent Information Regulator Proteins, Saccharomyces cerevisiae
  • Recombinant Fusion Proteins
  • Microscopy, Fluorescence
  • Protein Processing, Post-Translational
  • Mutation
  • Time Factors
  • Sumoylation
Sprache
eng
Medium
Electronic
Online publication date
2011
Paginierung
867 - 874
Datum der Veröffentlichung
2011
Status
Published
Datum der Datenerfassung
2011
Titel
The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
13

Datenquelle: Europe PubMed Central

Abstract
Budding yeast telomeres are reversibly bound at the nuclear envelope through two partially redundant pathways that involve the Sir2/3/4 silencing complex and the Yku70/80 heterodimer. To better understand how this is regulated, we studied the role of SUMOylation in telomere anchoring. We find that the PIAS-like SUMO E3 ligase Siz2 sumoylates both Yku70/80 and Sir4 in vivo and promotes telomere anchoring to the nuclear envelope. Remarkably, loss of Siz2 also provokes telomere extension in a telomerase-dependent manner that is epistatic with loss of the helicase Pif1. Consistent with our previously documented role for telomerase in anchorage, normal telomere anchoring in siz2 Δ is restored by PIF1 deletion. By live-cell imaging of a critically short telomere, we show that telomeres shift away from the nuclear envelope when elongating. We propose that SUMO-dependent association with the nuclear periphery restrains bound telomerase, whereas active elongation correlates with telomere release.
Date of acceptance
2011
Autoren
  • Helder C Ferreira
  • Brian Luke
  • Heiko Schober
  • Véronique Kalck
  • Joachim Lingner
  • Susan M Gasser
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/21666682
DOI
10.1038/ncb2263
eISSN
1476-4679
Funding acknowledgements
  • European Research Council: 232812
Ausgabe der Veröffentlichung
7
Zeitschrift
Nat Cell Biol
Schlüsselwörter
  • DNA Helicases
  • DNA-Binding Proteins
  • Microscopy, Fluorescence
  • Mutation
  • Nuclear Envelope
  • Protein Processing, Post-Translational
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Silent Information Regulator Proteins, Saccharomyces cerevisiae
  • Sumoylation
  • Telomerase
  • Telomere
  • Time Factors
Sprache
eng
Country
England
Paginierung
867 - 874
PII
ncb2263
Datum der Veröffentlichung
2011
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2011
Titel
The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
13

Datenquelle: PubMed

Abstract
Budding yeast telomeres are reversibly bound at the nuclear envelope through two partially redundant pathways that involve the Sir2/3/4 silencing complex and the Yku70/80 heterodimer. To better understand how this is regulated, we studied the role of SUMOylation in telomere anchoring. We find that the PIAS-like SUMO E3 ligase Siz2 sumoylates both Yku70/80 and Sir4 in vivo and promotes telomere anchoring to the nuclear envelope. Remarkably, loss of Siz2 also provokes telomere extension in a telomerase-dependent manner that is epistatic with loss of the helicase Pif1. Consistent with our previously documented role for telomerase in anchorage, normal telomere anchoring in siz2 \textgreekD is restored by PIF1 deletion. By live-cell imaging of a critically short telomere, we show that telomeres shift away from the nuclear envelope when elongating. We propose that SUMO-dependent association with the nuclear periphery restrains bound telomerase, whereas active elongation correlates with telomere release.
Autoren
  • Helder C Ferreira
  • Brian Luke
  • Heiko Schober
  • Véronique Kalck
  • Joachim Lingner
  • Susan M Gasser
DOI
10.1038/ncb2263
Zeitschrift
Nature cell biology
Notes
keywords: DNA Helicases/genetics/metabolism;DNA-Binding Proteins/genetics/metabolism;Microscopy, Fluorescence;Mutation;Nuclear Envelope/enzymology;Protein Processing, Post-Translational;Recombinant Fusion Proteins/metabolism;Saccharomyces cerevisiae Proteins/genetics/metabolism;Saccharomyces cerevisiae/enzymology/genetics;Silent Information Regulator Proteins, Saccharomyces cerevisiae/genetics/metabolism;Sumoylation;Telomerase/metabolism;Telomere/enzymology;Time Factors
Artikelnummer
7
Paginierung
867 - 874
Datum der Veröffentlichung
2011
Datum der Datenerfassung
2023
Titel
The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast
Sub types
  • article
Ausgabe der Zeitschrift
13

Datenquelle: Manual

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