The two- component system CroRS acts as a master regulator of cell envelope homeostasis to confer antimicrobial tolerance in the bacterial pathogen Enterococcus faecalis
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Francesca O Todd O Rose
- Rachel L Darnell
- Sali M Morris
- Olivia E Rose
- Olivia Paxie
- Georgia Campbell
- Gregory M Cook
- Susanne Gebhard
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:001033623900001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1111/mmi.15128
- eISSN
- 1365-2958
- Externe Identifier
- Clarivate Analytics Document Solution ID: U1ZX3
- PubMed Identifier: 37475106
- ISSN
- 0950-382X
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- MOLECULAR MICROBIOLOGY
- Schlüsselwörter
- bacterial
- cell wall
- drug resistance
- Enterococcus faecalis
- isoprenoids
- regulation
- RNA-seq
- tolerance
- Paginierung
- 408 - 424
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Titel
- The two- component system CroRS acts as a master regulator of cell envelope homeostasis to confer antimicrobial tolerance in the bacterial pathogen<i> Enterococcus</i><i> faecalis</i>
- Sub types
- Article
- Ausgabe der Zeitschrift
- 120
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Antimicrobial tolerance is the ability of a microbial population to survive, but not proliferate, during antimicrobial exposure. Significantly, it has been shown to precede the development of bona fide antimicrobial resistance. We have previously identified the two‐component system CroRS as a critical regulator of tolerance to antimicrobials like teixobactin in the bacterial pathogen <jats:italic>Enterococcus faecalis</jats:italic>. To understand the molecular mechanism of this tolerance, we have carried out RNA‐seq analyses in the <jats:italic>E. faecalis</jats:italic> wild‐type and isogenic <jats:italic>croRS</jats:italic> mutant to determine the teixobactin‐induced CroRS regulon. We identified a 132 gene CroRS regulon and demonstrate that CroRS upregulates biosynthesis of all major components of the enterococcal cell envelope in response to teixobactin. This suggests a coordinating role of this regulatory system in maintaining integrity of the multiple layers of the enterococcal envelope during antimicrobial stress, likely contributing to bacterial survival. Using experimental evolution, we observed that truncation of HppS, a key enzyme in the synthesis of the quinone electron carrier demethylmenaquinone, was sufficient to rescue tolerance in the <jats:italic>croRS</jats:italic> deletion strain. This highlights a key role for isoprenoid biosynthesis in antimicrobial tolerance in <jats:italic>E. faecalis</jats:italic>. Here, we propose a model of CroRS acting as a master regulator of cell envelope biogenesis and a gate‐keeper between isoprenoid biosynthesis and respiration to ensure tolerance against antimicrobial challenge.</jats:p>
- Autoren
- Francesca O Todd Rose
- Rachel L Darnell
- Sali M Morris
- Olivia E Rose
- Olivia Paxie
- Georgia Campbell
- Gregory M Cook
- Susanne Gebhard
- DOI
- 10.1111/mmi.15128
- eISSN
- 1365-2958
- ISSN
- 0950-382X
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Molecular Microbiology
- Sprache
- en
- Online publication date
- 2023
- Paginierung
- 408 - 424
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1111/mmi.15128
- Datum der Datenerfassung
- 2023
- Titel
- The two‐component system CroRS acts as a master regulator of cell envelope homeostasis to confer antimicrobial tolerance in the bacterial pathogen <i>Enterococcus faecalis</i>
- Ausgabe der Zeitschrift
- 120
Datenquelle: Crossref
- Abstract
- Antimicrobial tolerance is the ability of a microbial population to survive, but not proliferate, during antimicrobial exposure. Significantly, it has been shown to precede the development of bona fide antimicrobial resistance. We have previously identified the two-component system CroRS as a critical regulator of tolerance to antimicrobials like teixobactin in the bacterial pathogen Enterococcus faecalis. To understand the molecular mechanism of this tolerance, we have carried out RNA-seq analyses in the E. faecalis wild-type and isogenic ∆ croRS mutant to determine the teixobactin-induced CroRS regulon. We identified a 132 gene CroRS regulon and demonstrate that CroRS upregulates biosynthesis of all major components of the enterococcal cell envelope in response to teixobactin. This suggests a coordinating role of this regulatory system in maintaining integrity of the multiple layers of the enterococcal envelope during antimicrobial stress, likely contributing to bacterial survival. Using experimental evolution, we observed that truncation of HppS, a key enzyme in the synthesis of the quinone electron carrier demethylmenaquinone, was sufficient to rescue tolerance in the croRS deletion strain. This highlights a key role for isoprenoid biosynthesis in antimicrobial tolerance in E. faecalis. Here, we propose a model of CroRS acting as a master regulator of cell envelope biogenesis and a gate-keeper between isoprenoid biosynthesis and respiration to ensure tolerance against antimicrobial challenge.
- Addresses
- Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
- Autoren
- Francesca O Todd Rose
- Rachel L Darnell
- Sali M Morris
- Olivia E Rose
- Olivia Paxie
- Georgia Campbell
- Gregory M Cook
- Susanne Gebhard
- DOI
- 10.1111/mmi.15128
- eISSN
- 1365-2958
- Externe Identifier
- PubMed Identifier: 37475106
- PubMed Central ID: PMC10952268
- Funding acknowledgements
- Health Research Council of New Zealand: 20/213
- Maurice Wilkins Centre for Molecular Biodiscovery:
- Open access
- true
- ISSN
- 0950-382X
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Molecular microbiology
- Schlüsselwörter
- Enterococcus faecalis
- Terpenes
- Bacterial Proteins
- Anti-Infective Agents
- Anti-Bacterial Agents
- Microbial Sensitivity Tests
- Homeostasis
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2023
- Open access status
- Open Access
- Paginierung
- 408 - 424
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2023
- Titel
- The two-component system CroRS acts as a master regulator of cell envelope homeostasis to confer antimicrobial tolerance in the bacterial pathogen Enterococcus faecalis.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 120
Files
https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/mmi.15128 https://europepmc.org/articles/PMC10952268?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Antimicrobial tolerance is the ability of a microbial population to survive, but not proliferate, during antimicrobial exposure. Significantly, it has been shown to precede the development of bona fide antimicrobial resistance. We have previously identified the two-component system CroRS as a critical regulator of tolerance to antimicrobials like teixobactin in the bacterial pathogen Enterococcus faecalis. To understand the molecular mechanism of this tolerance, we have carried out RNA-seq analyses in the E. faecalis wild-type and isogenic ∆ croRS mutant to determine the teixobactin-induced CroRS regulon. We identified a 132 gene CroRS regulon and demonstrate that CroRS upregulates biosynthesis of all major components of the enterococcal cell envelope in response to teixobactin. This suggests a coordinating role of this regulatory system in maintaining integrity of the multiple layers of the enterococcal envelope during antimicrobial stress, likely contributing to bacterial survival. Using experimental evolution, we observed that truncation of HppS, a key enzyme in the synthesis of the quinone electron carrier demethylmenaquinone, was sufficient to rescue tolerance in the croRS deletion strain. This highlights a key role for isoprenoid biosynthesis in antimicrobial tolerance in E. faecalis. Here, we propose a model of CroRS acting as a master regulator of cell envelope biogenesis and a gate-keeper between isoprenoid biosynthesis and respiration to ensure tolerance against antimicrobial challenge.
- Date of acceptance
- 2023
- Autoren
- Francesca O Todd Rose
- Rachel L Darnell
- Sali M Morris
- Olivia E Rose
- Olivia Paxie
- Georgia Campbell
- Gregory M Cook
- Susanne Gebhard
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/37475106
- DOI
- 10.1111/mmi.15128
- eISSN
- 1365-2958
- Externe Identifier
- PubMed Central ID: PMC10952268
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Mol Microbiol
- Schlüsselwörter
- Enterococcus faecalis
- RNA-seq
- bacterial
- cell wall
- drug resistance
- isoprenoids
- regulation
- tolerance
- Anti-Bacterial Agents
- Enterococcus faecalis
- Bacterial Proteins
- Anti-Infective Agents
- Homeostasis
- Terpenes
- Microbial Sensitivity Tests
- Sprache
- eng
- Country
- England
- Paginierung
- 408 - 424
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2023
- Titel
- The two-component system CroRS acts as a master regulator of cell envelope homeostasis to confer antimicrobial tolerance in the bacterial pathogen Enterococcus faecalis.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 120
Datenquelle: PubMed
- Beziehungen:
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