In-Depth Profiling of Calcite Precipitation by Environmental Bacteria Reveals Fundamental Mechanistic Differences with Relevance to Application
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Bianca J Reeksting
- Timothy D Hoffmann
- Linzhen Tan
- Kevin Paine
- Susanne Gebhard
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000522978900015&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1128/AEM.02739-19
- eISSN
- 1098-5336
- Externe Identifier
- Clarivate Analytics Document Solution ID: KZ0RI
- PubMed Identifier: 31980427
- ISSN
- 0099-2240
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- Schlüsselwörter
- ureolysis
- self-healing concrete
- microbially induced calcite precipitation
- Bacillus
- Artikelnummer
- ARTN e02739-19
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Titel
- In-Depth Profiling of Calcite Precipitation by Environmental Bacteria Reveals Fundamental Mechanistic Differences with Relevance to Application
- Sub types
- Article
- Ausgabe der Zeitschrift
- 86
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:p>Biomineralization triggered by bacteria is important in the natural environment and has many applications in industry and in civil and geotechnical engineering. The diversity in biomineralization capabilities of environmental bacteria is, however, not well understood. This study surveyed environmental bacteria for their ability to precipitate calcium carbonate minerals and investigated both the mechanisms and the resulting crystals. We show that while urease activity leads to the fastest precipitation, it is by no means essential. Importantly, the same quantities of calcium carbonate are produced by nonureolytic bacteria, and the resulting crystals appear to have larger volumes and more organic components, which are likely beneficial in specific applications. Testing both precipitation mechanisms in a self-healing concrete application showed that nonureolytic bacteria delivered more robust results. Here, we performed a systematic study of the fundamental differences in biomineralization between environmental bacteria, and we provide important information for the design of bacterially based engineering solutions.</jats:p>
- Autoren
- Bianca J Reeksting
- Timothy D Hoffmann
- Linzhen Tan
- Kevin Paine
- Susanne Gebhard
- DOI
- 10.1128/aem.02739-19
- Editoren
- Robert M Kelly
- eISSN
- 1098-5336
- ISSN
- 0099-2240
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- Applied and Environmental Microbiology
- Sprache
- en
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Herausgeber
- American Society for Microbiology
- Herausgeber URL
- http://dx.doi.org/10.1128/aem.02739-19
- Datum der Datenerfassung
- 2022
- Titel
- In-Depth Profiling of Calcite Precipitation by Environmental Bacteria Reveals Fundamental Mechanistic Differences with Relevance to Application
- Ausgabe der Zeitschrift
- 86
Datenquelle: Crossref
- Abstract
- Microbially induced calcite precipitation (MICP) has not only helped to shape our planet's geological features but is also a promising technology to address environmental concerns in civil engineering applications. However, limited understanding of the biomineralization capacity of environmental bacteria impedes application. We therefore surveyed the environment for different mechanisms of precipitation across bacteria. The most fundamental difference was in ureolytic ability, where urease-positive bacteria caused rapid, widespread increases in pH, whereas nonureolytic strains produced such changes slowly and locally. These pH shifts correlated well with patterns of precipitation on solid medium. Strikingly, while both mechanisms led to high levels of precipitation, we observed clear differences in the precipitate. Ureolytic bacteria produced homogenous, inorganic fine crystals, whereas the crystals of nonureolytic strains were larger and had a mixed organic/inorganic composition. When representative strains were tested in application for crack healing in cement mortars, nonureolytic bacteria gave robust results, while ureolytic strains showed more variation. This may be explained by our observation that urease activity differed between growth conditions or by the different natures and therefore different material performances of the precipitates. Our results shed light on the breadth of biomineralization activity among environmental bacteria, an important step toward the rational design of bacterially based engineering solutions.<b>IMPORTANCE</b> Biomineralization triggered by bacteria is important in the natural environment and has many applications in industry and in civil and geotechnical engineering. The diversity in biomineralization capabilities of environmental bacteria is, however, not well understood. This study surveyed environmental bacteria for their ability to precipitate calcium carbonate minerals and investigated both the mechanisms and the resulting crystals. We show that while urease activity leads to the fastest precipitation, it is by no means essential. Importantly, the same quantities of calcium carbonate are produced by nonureolytic bacteria, and the resulting crystals appear to have larger volumes and more organic components, which are likely beneficial in specific applications. Testing both precipitation mechanisms in a self-healing concrete application showed that nonureolytic bacteria delivered more robust results. Here, we performed a systematic study of the fundamental differences in biomineralization between environmental bacteria, and we provide important information for the design of bacterially based engineering solutions.
- Addresses
- Department of Biology and Biochemistry, Milner Centre for Evolution, University of Bath, Bath, United Kingdom.
- Autoren
- Bianca J Reeksting
- Timothy D Hoffmann
- Linzhen Tan
- Kevin Paine
- Susanne Gebhard
- DOI
- 10.1128/aem.02739-19
- eISSN
- 1098-5336
- Externe Identifier
- PubMed Identifier: 31980427
- PubMed Central ID: PMC7082560
- Funding acknowledgements
- Engineering and Physical Sciences Research Council: EP/P02081X/1
- UK Research and Innovation | Engineering and Physical Sciences Research Council: EP/PO2081X/1
- Open access
- true
- ISSN
- 0099-2240
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- Applied and environmental microbiology
- Schlüsselwörter
- Bacteria
- Calcium Carbonate
- Environmental Microbiology
- Chemical Precipitation
- Sprache
- eng
- Medium
- Electronic-Print
- Online publication date
- 2020
- Open access status
- Open Access
- Paginierung
- e02739 - e02719
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2020
- Titel
- In-Depth Profiling of Calcite Precipitation by Environmental Bacteria Reveals Fundamental Mechanistic Differences with Relevance to Application.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 86
Files
https://europepmc.org/articles/PMC7082560?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Microbially induced calcite precipitation (MICP) has not only helped to shape our planet's geological features but is also a promising technology to address environmental concerns in civil engineering applications. However, limited understanding of the biomineralization capacity of environmental bacteria impedes application. We therefore surveyed the environment for different mechanisms of precipitation across bacteria. The most fundamental difference was in ureolytic ability, where urease-positive bacteria caused rapid, widespread increases in pH, whereas nonureolytic strains produced such changes slowly and locally. These pH shifts correlated well with patterns of precipitation on solid medium. Strikingly, while both mechanisms led to high levels of precipitation, we observed clear differences in the precipitate. Ureolytic bacteria produced homogenous, inorganic fine crystals, whereas the crystals of nonureolytic strains were larger and had a mixed organic/inorganic composition. When representative strains were tested in application for crack healing in cement mortars, nonureolytic bacteria gave robust results, while ureolytic strains showed more variation. This may be explained by our observation that urease activity differed between growth conditions or by the different natures and therefore different material performances of the precipitates. Our results shed light on the breadth of biomineralization activity among environmental bacteria, an important step toward the rational design of bacterially based engineering solutions.IMPORTANCE Biomineralization triggered by bacteria is important in the natural environment and has many applications in industry and in civil and geotechnical engineering. The diversity in biomineralization capabilities of environmental bacteria is, however, not well understood. This study surveyed environmental bacteria for their ability to precipitate calcium carbonate minerals and investigated both the mechanisms and the resulting crystals. We show that while urease activity leads to the fastest precipitation, it is by no means essential. Importantly, the same quantities of calcium carbonate are produced by nonureolytic bacteria, and the resulting crystals appear to have larger volumes and more organic components, which are likely beneficial in specific applications. Testing both precipitation mechanisms in a self-healing concrete application showed that nonureolytic bacteria delivered more robust results. Here, we performed a systematic study of the fundamental differences in biomineralization between environmental bacteria, and we provide important information for the design of bacterially based engineering solutions.
- Date of acceptance
- 2020
- Autoren
- Bianca J Reeksting
- Timothy D Hoffmann
- Linzhen Tan
- Kevin Paine
- Susanne Gebhard
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/31980427
- DOI
- 10.1128/AEM.02739-19
- eISSN
- 1098-5336
- Externe Identifier
- PubMed Central ID: PMC7082560
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- Appl Environ Microbiol
- Schlüsselwörter
- Bacillus
- microbially induced calcite precipitation
- self-healing concrete
- ureolysis
- Bacteria
- Calcium Carbonate
- Chemical Precipitation
- Environmental Microbiology
- Sprache
- eng
- Country
- United States
- PII
- AEM.02739-19
- Datum der Veröffentlichung
- 2020
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Titel
- In-Depth Profiling of Calcite Precipitation by Environmental Bacteria Reveals Fundamental Mechanistic Differences with Relevance to Application.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 86
Datenquelle: PubMed
- Beziehungen:
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