Coevolution of ABC Transporters and Two-Component Regulatory Systems as Resistance Modules against Antimicrobial Peptides in Firmicutes Bacteria
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Sebastian Dintner
- Anna Staron
- Evi Berchtold
- Tobias Petri
- Thorsten Mascher
- Susanne Gebhard
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000292698100018&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1128/JB.05175-11
- eISSN
- 1098-5530
- Externe Identifier
- Clarivate Analytics Document Solution ID: 791WF
- PubMed Identifier: 21665979
- ISSN
- 0021-9193
- Ausgabe der Veröffentlichung
- 15
- Zeitschrift
- JOURNAL OF BACTERIOLOGY
- Paginierung
- 3851 - 3862
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Titel
- Coevolution of ABC Transporters and Two-Component Regulatory Systems as Resistance Modules against Antimicrobial Peptides in <i>Firmicutes</i> Bacteria
- Sub types
- Article
- Ausgabe der Zeitschrift
- 193
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:title>ABSTRACT</jats:title> <jats:p> In <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Firmicutes</jats:named-content> bacteria, ATP-binding cassette (ABC) transporters have been recognized as important resistance determinants against antimicrobial peptides. Together with neighboring two-component systems (TCSs), which regulate their expression, they form specific detoxification modules. Both the transport permease and sensor kinase components show unusual domain architecture: the permeases contain a large extracellular domain, while the sensor kinases lack an obvious input domain. One of the best-characterized examples is the bacitracin resistance module BceRS-BceAB of <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Bacillus subtilis</jats:named-content> . Strikingly, in this system, the ABC transporter and TCS have an absolute mutual requirement for each other in both sensing of and resistance to bacitracin, suggesting a novel mode of signal transduction in which the transporter constitutes the actual sensor. We identified over 250 such BceAB-like ABC transporters in the current databases. They occurred almost exclusively in <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Firmicutes</jats:named-content> bacteria, and 80% of the transporters were associated with a BceRS-like TCS. Phylogenetic analyses of the permease and sensor kinase components revealed a tight evolutionary correlation. Our findings suggest a direct regulatory interaction between the ABC transporters and TCSs, mediating communication between both components. Based on their observed coclustering and conservation of response regulator binding sites, we could identify putative corresponding two-component systems for transporters lacking a regulatory system in their immediate neighborhood. Taken together, our results show that these types of ABC transporters and TCSs have coevolved to form self-sufficient detoxification modules against antimicrobial peptides, widely distributed among <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Firmicutes</jats:named-content> bacteria. </jats:p>
- Autoren
- Sebastian Dintner
- Anna Staroń
- Evi Berchtold
- Tobias Petri
- Thorsten Mascher
- Susanne Gebhard
- DOI
- 10.1128/jb.05175-11
- eISSN
- 1098-5530
- ISSN
- 0021-9193
- Ausgabe der Veröffentlichung
- 15
- Zeitschrift
- Journal of Bacteriology
- Sprache
- en
- Paginierung
- 3851 - 3862
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Herausgeber
- American Society for Microbiology
- Herausgeber URL
- http://dx.doi.org/10.1128/jb.05175-11
- Datum der Datenerfassung
- 2021
- Titel
- Coevolution of ABC Transporters and Two-Component Regulatory Systems as Resistance Modules against Antimicrobial Peptides in Firmicutes Bacteria
- Ausgabe der Zeitschrift
- 193
Datenquelle: Crossref
- Abstract
- In Firmicutes bacteria, ATP-binding cassette (ABC) transporters have been recognized as important resistance determinants against antimicrobial peptides. Together with neighboring two-component systems (TCSs), which regulate their expression, they form specific detoxification modules. Both the transport permease and sensor kinase components show unusual domain architecture: the permeases contain a large extracellular domain, while the sensor kinases lack an obvious input domain. One of the best-characterized examples is the bacitracin resistance module BceRS-BceAB of Bacillus subtilis. Strikingly, in this system, the ABC transporter and TCS have an absolute mutual requirement for each other in both sensing of and resistance to bacitracin, suggesting a novel mode of signal transduction in which the transporter constitutes the actual sensor. We identified over 250 such BceAB-like ABC transporters in the current databases. They occurred almost exclusively in Firmicutes bacteria, and 80% of the transporters were associated with a BceRS-like TCS. Phylogenetic analyses of the permease and sensor kinase components revealed a tight evolutionary correlation. Our findings suggest a direct regulatory interaction between the ABC transporters and TCSs, mediating communication between both components. Based on their observed coclustering and conservation of response regulator binding sites, we could identify putative corresponding two-component systems for transporters lacking a regulatory system in their immediate neighborhood. Taken together, our results show that these types of ABC transporters and TCSs have coevolved to form self-sufficient detoxification modules against antimicrobial peptides, widely distributed among Firmicutes bacteria.
- Addresses
- Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany.
- Autoren
- Sebastian Dintner
- Anna Staron
- Evi Berchtold
- Tobias Petri
- Thorsten Mascher
- Susanne Gebhard
- DOI
- 10.1128/jb.05175-11
- eISSN
- 1098-5530
- Externe Identifier
- PubMed Identifier: 21665979
- PubMed Central ID: PMC3147537
- Open access
- false
- ISSN
- 0021-9193
- Ausgabe der Veröffentlichung
- 15
- Zeitschrift
- Journal of bacteriology
- Schlüsselwörter
- Bacteria
- Bacillus subtilis
- Peptides
- Bacterial Proteins
- ATP-Binding Cassette Transporters
- Anti-Bacterial Agents
- Evolution, Molecular
- Phylogeny
- Drug Resistance, Bacterial
- Gene Expression Regulation, Bacterial
- Molecular Sequence Data
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2011
- Paginierung
- 3851 - 3862
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Datum der Datenerfassung
- 2011
- Titel
- Coevolution of ABC transporters and two-component regulatory systems as resistance modules against antimicrobial peptides in Firmicutes Bacteria.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 193
Files
https://jb.asm.org/content/jb/193/15/3851.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21665979/pdf/?tool=EBI https://europepmc.org/articles/PMC3147537?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- In Firmicutes bacteria, ATP-binding cassette (ABC) transporters have been recognized as important resistance determinants against antimicrobial peptides. Together with neighboring two-component systems (TCSs), which regulate their expression, they form specific detoxification modules. Both the transport permease and sensor kinase components show unusual domain architecture: the permeases contain a large extracellular domain, while the sensor kinases lack an obvious input domain. One of the best-characterized examples is the bacitracin resistance module BceRS-BceAB of Bacillus subtilis. Strikingly, in this system, the ABC transporter and TCS have an absolute mutual requirement for each other in both sensing of and resistance to bacitracin, suggesting a novel mode of signal transduction in which the transporter constitutes the actual sensor. We identified over 250 such BceAB-like ABC transporters in the current databases. They occurred almost exclusively in Firmicutes bacteria, and 80% of the transporters were associated with a BceRS-like TCS. Phylogenetic analyses of the permease and sensor kinase components revealed a tight evolutionary correlation. Our findings suggest a direct regulatory interaction between the ABC transporters and TCSs, mediating communication between both components. Based on their observed coclustering and conservation of response regulator binding sites, we could identify putative corresponding two-component systems for transporters lacking a regulatory system in their immediate neighborhood. Taken together, our results show that these types of ABC transporters and TCSs have coevolved to form self-sufficient detoxification modules against antimicrobial peptides, widely distributed among Firmicutes bacteria.
- Autoren
- Sebastian Dintner
- Anna Staron
- Evi Berchtold
- Tobias Petri
- Thorsten Mascher
- Susanne Gebhard
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/21665979
- DOI
- 10.1128/JB.05175-11
- eISSN
- 1098-5530
- Externe Identifier
- PubMed Central ID: PMC3147537
- Ausgabe der Veröffentlichung
- 15
- Zeitschrift
- J Bacteriol
- Schlüsselwörter
- ATP-Binding Cassette Transporters
- Anti-Bacterial Agents
- Bacillus subtilis
- Bacteria
- Bacterial Proteins
- Drug Resistance, Bacterial
- Evolution, Molecular
- Gene Expression Regulation, Bacterial
- Molecular Sequence Data
- Peptides
- Phylogeny
- Sprache
- eng
- Country
- United States
- Paginierung
- 3851 - 3862
- PII
- JB.05175-11
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2011
- Titel
- Coevolution of ABC transporters and two-component regulatory systems as resistance modules against antimicrobial peptides in Firmicutes Bacteria.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 193
Datenquelle: PubMed
- Beziehungen:
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