The low-affinity phosphate transporter PitA is dispensable for in vitro growth of Mycobacterium smegmatis

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000273125500001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1186/1471-2180-9-254
Externe Identifier
  • Clarivate Analytics Document Solution ID: 537PM
  • PubMed Identifier: 20003273
ISSN
1471-2180
Zeitschrift
BMC MICROBIOLOGY
Artikelnummer
ARTN 254
Datum der Veröffentlichung
2009
Status
Published
Titel
The low-affinity phosphate transporter PitA is dispensable for <i>in vitro</i> growth of <i>Mycobacterium smegmatis</i>
Sub types
  • Article
Ausgabe der Zeitschrift
9

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Autoren
DOI
10.1186/1471-2180-9-254
ISSN
1471-2180
Ausgabe der Veröffentlichung
1
Zeitschrift
BMC Microbiology
Sprache
en
Paginierung
254 - 254
Datum der Veröffentlichung
2009
Status
Published
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1186/1471-2180-9-254
Datum der Datenerfassung
2016
Titel
The low-affinity phosphate transporter PitA is dispensable for in vitro growth of Mycobacterium smegmatis
Ausgabe der Zeitschrift
9

Datenquelle: Crossref

Abstract
<h4>Background</h4>Mycobacteria have been shown to contain an apparent redundancy of high-affinity phosphate uptake systems, with two to four copies of such systems encoded in all mycobacterial genomes sequenced to date. In addition, all mycobacteria also contain at least one gene encoding the low-affinity phosphate transporter, Pit. No information is available on a Pit system from a Gram-positive microorganism, and the importance of this system in a background of multiple other phosphate transporters is unclear.<h4>Results</h4>The aim of this study was to determine the physiological role of the PitA phosphate transporter in Mycobacterium smegmatis. Expression of pitA was found to be constitutive under a variety of growth conditions. An unmarked deletion mutant in pitA of M. smegmatis was created. The deletion did not affect in vitro growth or phosphate uptake of M. smegmatis. Expression of the high-affinity transporters, PstSCAB and PhnDCE, was increased in the pitA deletion strain.<h4>Conclusion</h4>PitA is the only low-affinity phosphate transport system annotated in the genome of M. smegmatis. The lack of phenotype of the pitA deletion strain shows that this system is dispensable for in vitro growth of this organism. However, increased expression of the remaining phosphate transporters in the mutant indicates a compensatory mechanism and implies that PitA is indeed used for the uptake of phosphate in M. smegmatis.
Addresses
  • Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand. susanne.gebhard@bio.lmu.de
Autoren
DOI
10.1186/1471-2180-9-254
eISSN
1471-2180
Externe Identifier
  • PubMed Identifier: 20003273
  • PubMed Central ID: PMC2797804
Open access
true
ISSN
1471-2180
Zeitschrift
BMC microbiology
Schlüsselwörter
  • Mycobacterium smegmatis
  • Phosphates
  • Bacterial Proteins
  • Phosphate Transport Proteins
  • DNA, Bacterial
  • Genetic Complementation Test
  • Gene Expression Regulation, Bacterial
  • Sequence Deletion
Sprache
eng
Medium
Electronic
Online publication date
2009
Open access status
Open Access
Paginierung
254
Datum der Veröffentlichung
2009
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2009
Titel
The low-affinity phosphate transporter PitA is dispensable for in vitro growth of Mycobacterium smegmatis.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
9

Files

https://bmcmicrobiol.biomedcentral.com/counter/pdf/10.1186/1471-2180-9-254 https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20003273/pdf/?tool=EBI http://www.biomedcentral.com/content/pdf/1471-2180-9-254.pdf https://europepmc.org/articles/PMC2797804?pdf=render

Datenquelle: Europe PubMed Central

Abstract
BACKGROUND: Mycobacteria have been shown to contain an apparent redundancy of high-affinity phosphate uptake systems, with two to four copies of such systems encoded in all mycobacterial genomes sequenced to date. In addition, all mycobacteria also contain at least one gene encoding the low-affinity phosphate transporter, Pit. No information is available on a Pit system from a Gram-positive microorganism, and the importance of this system in a background of multiple other phosphate transporters is unclear. RESULTS: The aim of this study was to determine the physiological role of the PitA phosphate transporter in Mycobacterium smegmatis. Expression of pitA was found to be constitutive under a variety of growth conditions. An unmarked deletion mutant in pitA of M. smegmatis was created. The deletion did not affect in vitro growth or phosphate uptake of M. smegmatis. Expression of the high-affinity transporters, PstSCAB and PhnDCE, was increased in the pitA deletion strain. CONCLUSION: PitA is the only low-affinity phosphate transport system annotated in the genome of M. smegmatis. The lack of phenotype of the pitA deletion strain shows that this system is dispensable for in vitro growth of this organism. However, increased expression of the remaining phosphate transporters in the mutant indicates a compensatory mechanism and implies that PitA is indeed used for the uptake of phosphate in M. smegmatis.
Date of acceptance
2009
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/20003273
DOI
10.1186/1471-2180-9-254
eISSN
1471-2180
Externe Identifier
  • PubMed Central ID: PMC2797804
Zeitschrift
BMC Microbiol
Schlüsselwörter
  • Bacterial Proteins
  • DNA, Bacterial
  • Gene Expression Regulation, Bacterial
  • Genetic Complementation Test
  • Mycobacterium smegmatis
  • Phosphate Transport Proteins
  • Phosphates
  • Sequence Deletion
Sprache
eng
Country
England
Paginierung
254
PII
1471-2180-9-254
Datum der Veröffentlichung
2009
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2010
Titel
The low-affinity phosphate transporter PitA is dispensable for in vitro growth of Mycobacterium smegmatis.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
9

Datenquelle: PubMed

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