Tuning the pH-Switch of Supramolecular Polymer Carriers for siRNA to Physiologically Relevant pH

Abstract

AbstractThe preparation of histidine enriched dendritic peptide amphiphiles and their self‐assembly into multicomponent pH‐switchable supramolecular polymers is reported. Alternating histidine and phenylalanine peptide synthons allow the assembly/disassembly to be adjusted in a physiologically relevant range of pH 5.3–6.0. Coassembly of monomers equipped with dendritic tetraethylene glycol chains with monomers bearing peripheral primary amine groups leads to nanorods with a tunable cationic surface charge density. These surface functional supramolecular polycations are able to reversibly bind short interfering RNA (siRNA). The nanorod‐like supramolecular polymers, their complexation with siRNA, and the pH‐triggered assembly/disassembly of the supramolecular carriers are characterized via circular dichroism spectroscopy, gel electrophoresis, as well as transmission electron microscopy. Multicomponent supramolecular polymers represent a modular and promising strategy for applications as responsive carrier vehicles, codelivery strategies, and gene therapy.

Autoren

Patrick Ahlers
Hendrik Frisch
Regina Holm
Daniel Spitzer
Matthias Barz
Pol Besenius

DOI

10.1002/mabi.201700111

eISSN

1616-5195

ISSN

1616-5187

Ausgabe der Veröffentlichung

10

Zeitschrift

Macromolecular Bioscience

Sprache

en

Online publication date

2017

Datum der Veröffentlichung

2017

Status

Published

Herausgeber

Wiley

Herausgeber URL

http://dx.doi.org/10.1002/mabi.201700111

Datum der Datenerfassung

2023

Titel

Tuning the pH‐Switch of Supramolecular Polymer Carriers for siRNA to Physiologically Relevant pH

Ausgabe der Zeitschrift

17

Datenquelle: Crossref

Abstract

The preparation of histidine enriched dendritic peptide amphiphiles and their self-assembly into multicomponent pH-switchable supramolecular polymers is reported. Alternating histidine and phenylalanine peptide synthons allow the assembly/disassembly to be adjusted in a physiologically relevant range of pH 5.3-6.0. Coassembly of monomers equipped with dendritic tetraethylene glycol chains with monomers bearing peripheral primary amine groups leads to nanorods with a tunable cationic surface charge density. These surface functional supramolecular polycations are able to reversibly bind short interfering RNA (siRNA). The nanorod-like supramolecular polymers, their complexation with siRNA, and the pH-triggered assembly/disassembly of the supramolecular carriers are characterized via circular dichroism spectroscopy, gel electrophoresis, as well as transmission electron microscopy. Multicomponent supramolecular polymers represent a modular and promising strategy for applications as responsive carrier vehicles, codelivery strategies, and gene therapy.

Addresses

Institute of Organic Chemistry, Johannes Gutenberg-University Mainz, Duesbergweg 10-14, 55128, Mainz, Germany.

Autoren

Patrick Ahlers
Hendrik Frisch
Regina Holm
Daniel Spitzer
Matthias Barz
Pol Besenius

DOI

10.1002/mabi.201700111

eISSN

1616-5195

Externe Identifier

PubMed Identifier: 28671760

Funding acknowledgements

Deutsche Forschungsgemeinschaft: SFB 1066
Fonds der Chemischen Industrie:

Open access

false

ISSN

1616-5187

Ausgabe der Veröffentlichung

10

Zeitschrift

Macromolecular bioscience

Schlüsselwörter

Cations
Polyethylene Glycols
Phenylalanine
Histidine
Peptides
RNA, Small Interfering
Drug Carriers
Gene Transfer Techniques
Hydrogen-Ion Concentration
Nanotubes
Dendrimers
Static Electricity

Sprache

eng

Medium

Print-Electronic

Online publication date

2017

Datum der Veröffentlichung

2017

Status

Published

Datum der Datenerfassung

2017

Titel

Tuning the pH-Switch of Supramolecular Polymer Carriers for siRNA to Physiologically Relevant pH.

Sub types

Journal Article

Ausgabe der Zeitschrift

17

Datenquelle: Europe PubMed Central

Abstract

The preparation of histidine enriched dendritic peptide amphiphiles and their self-assembly into multicomponent pH-switchable supramolecular polymers is reported. Alternating histidine and phenylalanine peptide synthons allow the assembly/disassembly to be adjusted in a physiologically relevant range of pH 5.3-6.0. Coassembly of monomers equipped with dendritic tetraethylene glycol chains with monomers bearing peripheral primary amine groups leads to nanorods with a tunable cationic surface charge density. These surface functional supramolecular polycations are able to reversibly bind short interfering RNA (siRNA). The nanorod-like supramolecular polymers, their complexation with siRNA, and the pH-triggered assembly/disassembly of the supramolecular carriers are characterized via circular dichroism spectroscopy, gel electrophoresis, as well as transmission electron microscopy. Multicomponent supramolecular polymers represent a modular and promising strategy for applications as responsive carrier vehicles, codelivery strategies, and gene therapy.

Autoren

Patrick Ahlers
Hendrik Frisch
Regina Holm
Daniel Spitzer
Matthias Barz
Pol Besenius

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/28671760

DOI

10.1002/mabi.201700111

eISSN

1616-5195

Ausgabe der Veröffentlichung

10

Zeitschrift

Macromol Biosci

Schlüsselwörter

charge regulation
nanomedicine
pH-triggered release
self-assembly
stimuli-responsive
Cations
Dendrimers
Drug Carriers
Gene Transfer Techniques
Histidine
Hydrogen-Ion Concentration
Nanotubes
Peptides
Phenylalanine
Polyethylene Glycols
RNA, Small Interfering
Static Electricity

Sprache

eng

Country

Germany

Datum der Veröffentlichung

2017

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2018

Titel

Tuning the pH-Switch of Supramolecular Polymer Carriers for siRNA to Physiologically Relevant pH.

Sub types

Journal Article

Ausgabe der Zeitschrift

17

Datenquelle: PubMed

Tuning the pH-Switch of Supramolecular Polymer Carriers for siRNA to Physiologically Relevant pH

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