Synthetic MUC1 Antitumor Vaccine with Incorporated 2,3-Sialyl-T Carbohydrate Antigen Inducing Strong Immune Responses with Isotype Specificity

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • David Strassburger
  • Markus Glaffig
  • Natascha Stergiou
  • Sabrina Bialas
  • Pol Besenius
  • Edgar Schmitt
  • Horst Kunz
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000435857200008&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1002/cbic.201800148
eISSN
1439-7633
Externe Identifier
  • Clarivate Analytics Document Solution ID: GK1ET
  • PubMed Identifier: 29633523
ISSN
1439-4227
Ausgabe der Veröffentlichung
11
Zeitschrift
CHEMBIOCHEM
Schlüsselwörter
  • antitumor agents
  • cancer
  • glycopeptides
  • regioselective sialylation
  • synthetic vaccines
Paginierung
1142 - 1146
Datum der Veröffentlichung
2018
Status
Published
Titel
Synthetic MUC1 Antitumor Vaccine with Incorporated 2,3-Sialyl-T Carbohydrate Antigen Inducing Strong Immune Responses with Isotype Specificity
Sub types
  • Article
Ausgabe der Zeitschrift
19

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<jats:title>Abstract</jats:title><jats:p>The endothelial glycoprotein MUC1 is known to underlie alterations in cancer by means of aberrant glycosylation accompanied by changes in morphology. The heavily shortened glycans induce a collapse of the peptide backbone and enable accessibility of the latter to immune cells, rendering it a tumor‐associated antigen. Synthetic vaccines based on MUC1 tandem repeat motifs, comprising tumor‐associated 2,3‐sialyl‐T antigen, conjugated to the immunostimulating tetanus toxoid, are reported herein. Immunization with these vaccines in a simple water/oil emulsion produced a strong immune response in mice to which stimulation with complete Freund's adjuvant (CFA) was not superior. In both cases, high levels of IgG1 and IgG2a/b were induced in C57BL/6 mice. Additional glycosylation in the immunodominant PDTRP domain led to improved binding of the induced antisera to MCF‐7 breast tumor cells, compared with that of the monoglycosylated peptide vaccine.</jats:p>
Autoren
  • David Straßburger
  • Markus Glaffig
  • Natascha Stergiou
  • Sabrina Bialas
  • Pol Besenius
  • Edgar Schmitt
  • Horst Kunz
DOI
10.1002/cbic.201800148
eISSN
1439-7633
ISSN
1439-4227
Ausgabe der Veröffentlichung
11
Zeitschrift
ChemBioChem
Sprache
en
Online publication date
2018
Paginierung
1142 - 1146
Datum der Veröffentlichung
2018
Status
Published
Herausgeber
Wiley
Herausgeber URL
http://dx.doi.org/10.1002/cbic.201800148
Datum der Datenerfassung
2023
Titel
Synthetic MUC1 Antitumor Vaccine with Incorporated 2,3‐Sialyl‐T Carbohydrate Antigen Inducing Strong Immune Responses with Isotype Specificity
Ausgabe der Zeitschrift
19

Datenquelle: Crossref

Abstract
The endothelial glycoprotein MUC1 is known to underlie alterations in cancer by means of aberrant glycosylation accompanied by changes in morphology. The heavily shortened glycans induce a collapse of the peptide backbone and enable accessibility of the latter to immune cells, rendering it a tumor-associated antigen. Synthetic vaccines based on MUC1 tandem repeat motifs, comprising tumor-associated 2,3-sialyl-T antigen, conjugated to the immunostimulating tetanus toxoid, are reported herein. Immunization with these vaccines in a simple water/oil emulsion produced a strong immune response in mice to which stimulation with complete Freund's adjuvant (CFA) was not superior. In both cases, high levels of IgG1 and IgG2a/b were induced in C57BL/6 mice. Additional glycosylation in the immunodominant PDTRP domain led to improved binding of the induced antisera to MCF-7 breast tumor cells, compared with that of the monoglycosylated peptide vaccine.
Addresses
  • Institut für Organische Chemie, Johannes Gutenberg-Universität Mainz, Duesbergweg 10-14, 55128, Mainz, Germany.
Autoren
  • David Straßburger
  • Markus Glaffig
  • Natascha Stergiou
  • Sabrina Bialas
  • Pol Besenius
  • Edgar Schmitt
  • Horst Kunz
DOI
10.1002/cbic.201800148
eISSN
1439-7633
Externe Identifier
  • PubMed Identifier: 29633523
Funding acknowledgements
  • Deutsche Forschungsgemeinschaft: SFB 1066
Open access
false
ISSN
1439-4227
Zeitschrift
Chembiochem : a European journal of chemical biology
Sprache
eng
Medium
Print-Electronic
Online publication date
2018
Datum der Veröffentlichung
2018
Status
Published
Datum der Datenerfassung
2018
Titel
Synthetic MUC1 Antitumor Vaccine with Incorporated 2,3-Sialyl-T Carbohydrate Antigen Inducing Strong Immune Responses with Isotype Specificity.
Sub types
  • Journal Article

Datenquelle: Europe PubMed Central

Abstract
The endothelial glycoprotein MUC1 is known to underlie alterations in cancer by means of aberrant glycosylation accompanied by changes in morphology. The heavily shortened glycans induce a collapse of the peptide backbone and enable accessibility of the latter to immune cells, rendering it a tumor-associated antigen. Synthetic vaccines based on MUC1 tandem repeat motifs, comprising tumor-associated 2,3-sialyl-T antigen, conjugated to the immunostimulating tetanus toxoid, are reported herein. Immunization with these vaccines in a simple water/oil emulsion produced a strong immune response in mice to which stimulation with complete Freund's adjuvant (CFA) was not superior. In both cases, high levels of IgG1 and IgG2a/b were induced in C57BL/6 mice. Additional glycosylation in the immunodominant PDTRP domain led to improved binding of the induced antisera to MCF-7 breast tumor cells, compared with that of the monoglycosylated peptide vaccine.
Autoren
  • David Straßburger
  • Markus Glaffig
  • Natascha Stergiou
  • Sabrina Bialas
  • Pol Besenius
  • Edgar Schmitt
  • Horst Kunz
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/29633523
DOI
10.1002/cbic.201800148
eISSN
1439-7633
Zeitschrift
Chembiochem
Schlüsselwörter
  • antitumor agents
  • cancer
  • glycopeptides
  • regioselective sialylation
  • synthetic vaccines
Sprache
eng
Country
Germany
Datum der Veröffentlichung
2018
Status
Published online
Titel
Synthetic MUC1 Antitumor Vaccine with Incorporated 2,3-Sialyl-T Carbohydrate Antigen Inducing Strong Immune Responses with Isotype Specificity.
Sub types
  • Journal Article

Datenquelle: PubMed

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