Mannose-Decorated Multicomponent Supramolecular Polymers Trigger Effective Uptake into Antigen-Presenting Cells

Abstract

AbstractA modular route to prepare functional self‐assembling dendritic peptide amphiphiles decorated with mannosides, to effectively target antigen‐presenting cells, such as macrophages, is reported. The monomeric building blocks were equipped with tetra(ethylene glycol)s (TEGs) or labeled with a Cy3 fluorescent probe. Experiments on the uptake of the multifunctional supramolecular particles into murine macrophages (Mφs) were monitored by confocal microscopy and fluorescence‐activated cell sorting. Mannose‐decorated supramolecular polymers trigger a significantly higher cellular uptake and distribution, relative to TEG carrying bare polymers. No cytotoxicity or negative impact on cytokine production of the treated Mφs was observed, which emphasized their biocompatibility. The modular nature of the multicomponent supramolecular polymer coassembly protocol is a promising platform to develop fully synthetic multifunctional vaccines, for example, in cancer immunotherapy.

Autoren

David Straßburger
Natascha Stergiou
Moritz Urschbach
Hajime Yurugi
Daniel Spitzer
Dieter Schollmeyer
Edgar Schmitt
Pol Besenius

DOI

10.1002/cbic.201800114

eISSN

1439-7633

ISSN

1439-4227

Ausgabe der Veröffentlichung

9

Zeitschrift

ChemBioChem

Sprache

en

Online publication date

2018

Paginierung

912 - 916

Datum der Veröffentlichung

2018

Status

Published

Herausgeber

Wiley

Herausgeber URL

http://dx.doi.org/10.1002/cbic.201800114

Datum der Datenerfassung

2023

Titel

Mannose‐Decorated Multicomponent Supramolecular Polymers Trigger Effective Uptake into Antigen‐Presenting Cells

Ausgabe der Zeitschrift

19

Datenquelle: Crossref

Abstract

A modular route to prepare functional self-assembling dendritic peptide amphiphiles decorated with mannosides, to effectively target antigen-presenting cells, such as macrophages, is reported. The monomeric building blocks were equipped with tetra(ethylene glycol)s (TEGs) or labeled with a Cy3 fluorescent probe. Experiments on the uptake of the multifunctional supramolecular particles into murine macrophages (Mφs) were monitored by confocal microscopy and fluorescence-activated cell sorting. Mannose-decorated supramolecular polymers trigger a significantly higher cellular uptake and distribution, relative to TEG carrying bare polymers. No cytotoxicity or negative impact on cytokine production of the treated Mφs was observed, which emphasized their biocompatibility. The modular nature of the multicomponent supramolecular polymer coassembly protocol is a promising platform to develop fully synthetic multifunctional vaccines, for example, in cancer immunotherapy.

Addresses

Institute of Organic Chemistry, Johannes Gutenberg-University Mainz, Duesbergweg 10-14, 55128, Mainz, Germany.

Autoren

David Straßburger
Natascha Stergiou
Moritz Urschbach
Hajime Yurugi
Daniel Spitzer
Dieter Schollmeyer
Edgar Schmitt
Pol Besenius

DOI

10.1002/cbic.201800114

eISSN

1439-7633

Externe Identifier

PubMed Identifier: 29486092

Funding acknowledgements

Deutsche Forschungsgemeinschaft: SFB 1066

Open access

false

ISSN

1439-4227

Ausgabe der Veröffentlichung

9

Zeitschrift

Chembiochem : a European journal of chemical biology

Schlüsselwörter

Antigen-Presenting Cells
Cells, Cultured
Macrophages
Animals
Mice
Polyethylene Glycols
Carbocyanines
Mannosides
Peptides
Fluorescent Dyes
Surface-Active Agents
Microscopy, Confocal
Biological Transport
Models, Molecular
Dendrimers

Sprache

eng

Medium

Print-Electronic

Online publication date

2018

Paginierung

912 - 916

Datum der Veröffentlichung

2018

Status

Published

Datum der Datenerfassung

2018

Titel

Mannose-Decorated Multicomponent Supramolecular Polymers Trigger Effective Uptake into Antigen-Presenting Cells.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

19

Datenquelle: Europe PubMed Central

Abstract

A modular route to prepare functional self-assembling dendritic peptide amphiphiles decorated with mannosides, to effectively target antigen-presenting cells, such as macrophages, is reported. The monomeric building blocks were equipped with tetra(ethylene glycol)s (TEGs) or labeled with a Cy3 fluorescent probe. Experiments on the uptake of the multifunctional supramolecular particles into murine macrophages (Mφs) were monitored by confocal microscopy and fluorescence-activated cell sorting. Mannose-decorated supramolecular polymers trigger a significantly higher cellular uptake and distribution, relative to TEG carrying bare polymers. No cytotoxicity or negative impact on cytokine production of the treated Mφs was observed, which emphasized their biocompatibility. The modular nature of the multicomponent supramolecular polymer coassembly protocol is a promising platform to develop fully synthetic multifunctional vaccines, for example, in cancer immunotherapy.

Autoren

David Straßburger
Natascha Stergiou
Moritz Urschbach
Hajime Yurugi
Daniel Spitzer
Dieter Schollmeyer
Edgar Schmitt
Pol Besenius

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/29486092

DOI

10.1002/cbic.201800114

eISSN

1439-7633

Ausgabe der Veröffentlichung

9

Zeitschrift

Chembiochem

Schlüsselwörter

amphiphiles
peptides
polymers
self-assembly
supramolecular chemistry
Animals
Antigen-Presenting Cells
Biological Transport
Carbocyanines
Cells, Cultured
Dendrimers
Fluorescent Dyes
Macrophages
Mannosides
Mice
Microscopy, Confocal
Models, Molecular
Peptides
Polyethylene Glycols
Surface-Active Agents

Sprache

eng

Country

Germany

Paginierung

912 - 916

Datum der Veröffentlichung

2018

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2019

Titel

Mannose-Decorated Multicomponent Supramolecular Polymers Trigger Effective Uptake into Antigen-Presenting Cells.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

19

Datenquelle: PubMed

Mannose-Decorated Multicomponent Supramolecular Polymers Trigger Effective Uptake into Antigen-Presenting Cells

Werkzeuge