Interkingdom Signaling of the Insect Pathogen Photorhabdus luminescens with Plants Via the LuxR solo SdiA
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Nazzareno Dominelli
- Alice Regaiolo
- Leon Willy
- Ralf Heermann
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000978432200001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/microorganisms11040890
- eISSN
- 2076-2607
- Externe Identifier
- Clarivate Analytics Document Solution ID: E9CH8
- PubMed Identifier: 37110313
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- MICROORGANISMS
- Schlüsselwörter
- entomopathogenic bacteria
- quorum sensing
- bacteria-plant interaction
- AidA
- LuxR-type receptor
- Artikelnummer
- ARTN 890
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Titel
- Interkingdom Signaling of the Insect Pathogen <i>Photorhabdus luminescens</i> with Plants Via the LuxR solo SdiA
- Sub types
- Article
- Ausgabe der Zeitschrift
- 11
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:p>In bacteria, group-coordinated behavior such as biofilm formation or virulence are often mediated via cell–cell communication, a process referred to as quorum sensing (QS). The canonical QS system of Gram-negative bacteria uses N-acyl homoserine lactones (AHLs) as communication molecules, which are produced by LuxI-type synthases and sensed by cognate LuxR-type receptors. These receptors act as transcriptional regulators controlling the expression of specific genes. Some bacteria harbor LuxR-type receptors lacking a cognate LuxI-type synthases, designated as LuxR solos. Among many other LuxR solos, the entomopathogenic enteric bacterium Photorhabdus luminescens harbors a SdiA-like LuxR solo containing an AHL signal-binding domain, for which a respective signal molecule and target genes have not been identified yet. Here we performed SPR analysis to demonstrate that SdiA acts as a bidirectional regulator of transcription, tightly controlling its own expression and the adjacent PluDJC_01670 (aidA) gene in P. luminescens, a gene supposed to be involved in the colonization of eukaryotes. Via qPCR we could further determine that in sdiA deletion mutant strains, aidA is upregulated, indicating that SdiA negatively affects expression of aidA. Furthermore, the ΔsdiA deletion mutant exhibited differences in biofilm formation and motility compared with the wild-type. Finally, using nanoDSF analysis we could identify putative binding ability of SdiA towards diverse AHLs, but also to plant-derived signals, modulating the DNA-binding capacity of SdiA, suggesting that this LuxR solo acts as an important player in interkingdom signaling between P. luminescens and plants.</jats:p>
- Autoren
- Nazzareno Dominelli
- Alice Regaiolo
- Leon Willy
- Ralf Heermann
- DOI
- 10.3390/microorganisms11040890
- eISSN
- 2076-2607
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Microorganisms
- Sprache
- en
- Online publication date
- 2023
- Paginierung
- 890 - 890
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/microorganisms11040890
- Datum der Datenerfassung
- 2023
- Titel
- Interkingdom Signaling of the Insect Pathogen Photorhabdus luminescens with Plants Via the LuxR solo SdiA
- Ausgabe der Zeitschrift
- 11
Datenquelle: Crossref
- Abstract
- In bacteria, group-coordinated behavior such as biofilm formation or virulence are often mediated via cell-cell communication, a process referred to as quorum sensing (QS). The canonical QS system of Gram-negative bacteria uses <i>N</i>-acyl homoserine lactones (AHLs) as communication molecules, which are produced by LuxI-type synthases and sensed by cognate LuxR-type receptors. These receptors act as transcriptional regulators controlling the expression of specific genes. Some bacteria harbor LuxR-type receptors lacking a cognate LuxI-type synthases, designated as LuxR solos. Among many other LuxR solos, the entomopathogenic enteric bacterium <i>Photorhabdus luminescens</i> harbors a SdiA-like LuxR solo containing an AHL signal-binding domain, for which a respective signal molecule and target genes have not been identified yet. Here we performed SPR analysis to demonstrate that SdiA acts as a bidirectional regulator of transcription, tightly controlling its own expression and the adjacent <i>PluDJC_01670</i> (<i>aidA</i>) gene in <i>P. luminescens,</i> a gene supposed to be involved in the colonization of eukaryotes. Via qPCR we could further determine that in <i>sdiA</i> deletion mutant strains, <i>aidA</i> is upregulated, indicating that SdiA negatively affects expression of <i>aidA</i>. Furthermore, the Δ<i>sdiA</i> deletion mutant exhibited differences in biofilm formation and motility compared with the wild-type. Finally, using nanoDSF analysis we could identify putative binding ability of SdiA towards diverse AHLs, but also to plant-derived signals, modulating the DNA-binding capacity of SdiA, suggesting that this LuxR solo acts as an important player in interkingdom signaling between <i>P. luminescens</i> and plants.
- Addresses
- Institute of Molecular Physiology (imP), Johannes-Gutenberg-University Mainz, Biocenter II, Microbiology and Biotechnology, Hanns-Dieter-Hüsch-Weg 17, 55128 Mainz, Germany.
- Autoren
- Nazzareno Dominelli
- Alice Regaiolo
- Leon Willy
- Ralf Heermann
- DOI
- 10.3390/microorganisms11040890
- eISSN
- 2076-2607
- Externe Identifier
- PubMed Identifier: 37110313
- PubMed Central ID: PMC10143992
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: INST 247/992-1 FUGG
- Open access
- true
- ISSN
- 2076-2607
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Microorganisms
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2023
- Open access status
- Open Access
- Paginierung
- 890
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2023
- Titel
- Interkingdom Signaling of the Insect Pathogen <i>Photorhabdus luminescens</i> with Plants Via the LuxR solo SdiA.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 11
Files
https://www.mdpi.com/2076-2607/11/4/890/pdf?version=1680150094 https://europepmc.org/articles/PMC10143992?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- In bacteria, group-coordinated behavior such as biofilm formation or virulence are often mediated via cell-cell communication, a process referred to as quorum sensing (QS). The canonical QS system of Gram-negative bacteria uses N-acyl homoserine lactones (AHLs) as communication molecules, which are produced by LuxI-type synthases and sensed by cognate LuxR-type receptors. These receptors act as transcriptional regulators controlling the expression of specific genes. Some bacteria harbor LuxR-type receptors lacking a cognate LuxI-type synthases, designated as LuxR solos. Among many other LuxR solos, the entomopathogenic enteric bacterium Photorhabdus luminescens harbors a SdiA-like LuxR solo containing an AHL signal-binding domain, for which a respective signal molecule and target genes have not been identified yet. Here we performed SPR analysis to demonstrate that SdiA acts as a bidirectional regulator of transcription, tightly controlling its own expression and the adjacent PluDJC_01670 (aidA) gene in P. luminescens, a gene supposed to be involved in the colonization of eukaryotes. Via qPCR we could further determine that in sdiA deletion mutant strains, aidA is upregulated, indicating that SdiA negatively affects expression of aidA. Furthermore, the ΔsdiA deletion mutant exhibited differences in biofilm formation and motility compared with the wild-type. Finally, using nanoDSF analysis we could identify putative binding ability of SdiA towards diverse AHLs, but also to plant-derived signals, modulating the DNA-binding capacity of SdiA, suggesting that this LuxR solo acts as an important player in interkingdom signaling between P. luminescens and plants.
- Date of acceptance
- 2023
- Autoren
- Nazzareno Dominelli
- Alice Regaiolo
- Leon Willy
- Ralf Heermann
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/37110313
- DOI
- 10.3390/microorganisms11040890
- Externe Identifier
- PubMed Central ID: PMC10143992
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: INST 247/992-1 FUGG
- ISSN
- 2076-2607
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Microorganisms
- Schlüsselwörter
- AidA
- LuxR-type receptor
- bacteria–plant interaction
- entomopathogenic bacteria
- quorum sensing
- Sprache
- eng
- Country
- Switzerland
- PII
- microorganisms11040890
- Datum der Veröffentlichung
- 2023
- Status
- Published online
- Titel
- Interkingdom Signaling of the Insect Pathogen Photorhabdus luminescens with Plants Via the LuxR solo SdiA.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 11
Datenquelle: PubMed
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