High-throughput sequencing analysis reveals genomic similarity in phenotypic heterogeneous Photorhabdus luminescens cell populations

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Nazzareno Dominelli
  • Heidi Yoko Jaeger
  • Angela Langer
  • Andreas Brachmann
  • Ralf Heermann
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000802331400001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1186/s13213-022-01677-5
eISSN
1869-2044
Externe Identifier
  • Clarivate Analytics Document Solution ID: 1P9OT
ISSN
1590-4261
Ausgabe der Veröffentlichung
1
Zeitschrift
ANNALS OF MICROBIOLOGY
Schlüsselwörter
  • Phenotypic heterogeneity
  • Genome analysis
  • Entomopathogenic bacteria
Artikelnummer
ARTN 20
Datum der Veröffentlichung
2022
Status
Published
Titel
High-throughput sequencing analysis reveals genomic similarity in phenotypic heterogeneous <i>Photorhabdus luminescens</i> cell populations
Sub types
  • Article
Ausgabe der Zeitschrift
72

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<jats:title>Abstract</jats:title><jats:sec> <jats:title>Purpose</jats:title> <jats:p>Phenotypic heterogeneity occurs in many bacterial populations: single cells of the same species display different phenotypes, despite being genetically identical. The Gram-negative entomopathogenic bacterium <jats:italic>Photorhabdus luminescens</jats:italic> is an excellent example to investigate bacterial phenotypic heterogeneity. Its dualistic life cycle includes a symbiotic stage interacting with entomopathogenic nematodes (EPNs) and a pathogenic stage killing insect larvae. <jats:italic>P. luminescens</jats:italic> appears in two phenotypically different cell forms: the primary (1°) and the secondary (2°) cell variants. While 1° cells are bioluminescent, pigmented, and produce a huge set of secondary metabolites, 2° cells lack all these phenotypes. The main difference between both phenotypic variants is that only 1° cells can undergo symbiosis with EPNs, a phenotype that is absent from 2° cells. Recent comparative transcriptome analysis revealed that genes mediating 1° cell-specific traits are modulated differently in 2° cells. Although it was previously suggested that heterogeneity in <jats:italic>P. luminescens</jats:italic> cells cultures is not genetically mediated by, e.g., larger rearrangements in the genome, the genetic similarity of both cell variants has not clearly been demonstrated yet.</jats:p> </jats:sec><jats:sec> <jats:title>Methods</jats:title> <jats:p>Here, we analyzed the genomes of both 1° and 2° cells by genome sequencing of each six single 1° and 2° clones that emerged from a single 1° clone after prolonged growth. Using different bioinformatics tools, the sequence data were analyzed for clustered point mutations or genetic rearrangements with respect to the respective phenotypic variant.</jats:p> </jats:sec><jats:sec> <jats:title>Result</jats:title> <jats:p>We demonstrate that isolated clones of 2° cells that switched from the 1° cell state do not display any noticeable mutation and do not genetically differ from 1° cells.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p>In summary, we show that the phenotypic differences in <jats:italic>P. luminescens</jats:italic> cell cultures are obviously not caused by mutations or genetic rearrangements in the genome but truly emerge from phenotypic heterogeneity.</jats:p> </jats:sec>
Autoren
  • Nazzareno Dominelli
  • Heidi Yoko Jäger
  • Angela Langer
  • Andreas Brachmann
  • Ralf Heermann
DOI
10.1186/s13213-022-01677-5
eISSN
1869-2044
ISSN
1590-4261
Ausgabe der Veröffentlichung
1
Zeitschrift
Annals of Microbiology
Sprache
en
Artikelnummer
20
Online publication date
2022
Datum der Veröffentlichung
2022
Status
Published
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1186/s13213-022-01677-5
Datum der Datenerfassung
2022
Titel
High-throughput sequencing analysis reveals genomic similarity in phenotypic heterogeneous Photorhabdus luminescens cell populations
Ausgabe der Zeitschrift
72

Datenquelle: Crossref

Author's licence
CC-BY
Autoren
  • Nazzareno Dominelli
  • Heidi Yoko Jäger
  • Angela Langer
  • Andreas Brachmann
  • Ralf Heermann
Hosting institution
Universitätsbibliothek Mainz
Sammlungen
  • DFG-491381577-G
Resource version
Published version
DOI
10.1186/s13213-022-01677-5
Funding acknowledgements
  • Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 491381577
File(s) embargoed
false
Open access
true
ISSN
1869-2044
Zeitschrift
Annals of microbiology
Schlüsselwörter
  • 570 Biowissenschaften
  • 570 Life sciences
Sprache
eng
Open access status
Open Access
Paginierung
20
Datum der Veröffentlichung
2022
Public URL
https://openscience.ub.uni-mainz.de/handle/20.500.12030/8182
Herausgeber
Springer Nature
Datum der Datenerfassung
2022
Datum, an dem der Datensatz öffentlich gemacht wurde
2022
Zugang
Public
Titel
High-throughput sequencing analysis reveals genomic similarity in phenotypic heterogeneous Photorhabdus luminescens cell populations
Ausgabe der Zeitschrift
72

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