Pilocarpine toxicity in retinal ganglion cells

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • CK Vorwerk
  • P Simon
  • M Gorla
  • W Katowitz
  • D Zurakowski
  • LA Levin
  • EB Dreyer
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000078849200036&DestLinkType=FullRecord&DestApp=WOS_CPL
eISSN
1552-5783
Externe Identifier
  • Clarivate Analytics Document Solution ID: 171EH
  • PubMed Identifier: 10067991
ISSN
0146-0404
Ausgabe der Veröffentlichung
3
Zeitschrift
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
Paginierung
813 - 816
Datum der Veröffentlichung
1999
Status
Published
Titel
Pilocarpine toxicity in retinal ganglion cells
Sub types
  • Article
Ausgabe der Zeitschrift
40

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<h4>Purpose</h4>Muscarinic agents reduce intraocular pressure by enhancing aqueous outflow, probably by stimulating ciliary muscle contraction. However, pilocarpine is a well characterized neurotoxin and is widely used to generate animal seizure models. It was therefore investigated whether pilocarpine was also toxic to retinal ganglion cells.<h4>Methods</h4>Dissociated whole retinal preparations were prepared from postnatal day 16 to 19 rats. Retinal ganglion cells had been previously back-labeled with a fluorescent tracer. Retinal cells were incubated with pilocarpine, lithium, and inositol derivatives, and viability of the retrogradely labeled retinal ganglion cells was assayed after 24 hours.<h4>Results</h4>Pilocarpine was toxic to retinal ganglion cells in a dose-dependent fashion. This toxicity was potentiated by lithium and blocked by epi- and myo-inositol.<h4>Conclusions</h4>Pilocarpine is toxic to retinal ganglion cells in a mixed culture assay. This toxicity appears to depend on the inositol pathway and is similar to its mode of action in other neurons. However, 0.4 mM pilocarpine (the lowest concentration that did not affect ganglion cell survival) is roughly 1000-fold higher than the vitreal concentration and 20-fold higher than the scleral concentration that can be obtained with topical administration of 2% pilocarpine in the rabbit eye.
Addresses
  • The Department of Ophthalmology, Veterans Administration, and the University of Pennsylvania, Philadelphia 19104, USA.
Autoren
  • CK Vorwerk
  • P Simon
  • M Gorla
  • W Katowitz
  • D Zurakowski
  • LA Levin
  • EB Dreyer
eISSN
1552-5783
Externe Identifier
  • PubMed Identifier: 10067991
Funding acknowledgements
  • NEI NIH HHS: R01-EY10009
Open access
false
ISSN
0146-0404
Ausgabe der Veröffentlichung
3
Zeitschrift
Investigative ophthalmology & visual science
Schlüsselwörter
  • Retinal Ganglion Cells
  • Cells, Cultured
  • Animals
  • Rats
  • Rats, Long-Evans
  • Lithium
  • Inositol
  • Pilocarpine
  • Cell Survival
  • Dose-Response Relationship, Drug
  • Drug Synergism
Sprache
eng
Medium
Print
Paginierung
813 - 816
Datum der Veröffentlichung
1999
Status
Published
Datum der Datenerfassung
1999
Titel
Pilocarpine toxicity in retinal ganglion cells.
Sub types
  • Research Support, U.S. Gov't, P.H.S.
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
40

Datenquelle: Europe PubMed Central

Abstract
PURPOSE: Muscarinic agents reduce intraocular pressure by enhancing aqueous outflow, probably by stimulating ciliary muscle contraction. However, pilocarpine is a well characterized neurotoxin and is widely used to generate animal seizure models. It was therefore investigated whether pilocarpine was also toxic to retinal ganglion cells. METHODS: Dissociated whole retinal preparations were prepared from postnatal day 16 to 19 rats. Retinal ganglion cells had been previously back-labeled with a fluorescent tracer. Retinal cells were incubated with pilocarpine, lithium, and inositol derivatives, and viability of the retrogradely labeled retinal ganglion cells was assayed after 24 hours. RESULTS: Pilocarpine was toxic to retinal ganglion cells in a dose-dependent fashion. This toxicity was potentiated by lithium and blocked by epi- and myo-inositol. CONCLUSIONS: Pilocarpine is toxic to retinal ganglion cells in a mixed culture assay. This toxicity appears to depend on the inositol pathway and is similar to its mode of action in other neurons. However, 0.4 mM pilocarpine (the lowest concentration that did not affect ganglion cell survival) is roughly 1000-fold higher than the vitreal concentration and 20-fold higher than the scleral concentration that can be obtained with topical administration of 2% pilocarpine in the rabbit eye.
Autoren
  • CK Vorwerk
  • P Simon
  • M Gorla
  • W Katowitz
  • D Zurakowski
  • LA Levin
  • EB Dreyer
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/10067991
Funding acknowledgements
  • NEI NIH HHS: R01-EY10009
ISSN
0146-0404
Ausgabe der Veröffentlichung
3
Zeitschrift
Invest Ophthalmol Vis Sci
Schlüsselwörter
  • Animals
  • Cell Survival
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Drug Synergism
  • Inositol
  • Lithium
  • Pilocarpine
  • Rats
  • Rats, Long-Evans
  • Retinal Ganglion Cells
Sprache
eng
Country
United States
Paginierung
813 - 816
Datum der Veröffentlichung
1999
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
1999
Titel
Pilocarpine toxicity in retinal ganglion cells.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
Ausgabe der Zeitschrift
40

Datenquelle: PubMed

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