Early transcriptional changes of retinal and choroidal TGFβ-2, RALDH-2, and ZENK following imposed positive and negative defocus in chickens

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • P Simon
  • M Feldkaemper
  • M Bitzer
  • S Ohngemach
  • F Schaeffel
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000223642300001&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: 850VK
  • PubMed Identifier: 15343149
ISSN
1090-0535
Ausgabe der Veröffentlichung
71
Zeitschrift
MOLECULAR VISION
Paginierung
588 - 597
Datum der Veröffentlichung
2004
Status
Published
Titel
Early transcriptional changes of retinal and choroidal <i>TGF</i>β-2, RALDH-2, and ZENK following imposed positive and negative defocus in chickens
Sub types
  • Article
Ausgabe der Zeitschrift
10

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<h4>Purpose</h4>Imposing defocus to the retina results in compensatory changes of axial eye growth. It is not clear which factors initially contribute to this process and whether they act on the post-translational, translational, or transcriptional level. We have measured early changes in mRNA levels, in response to imposed negative and positive defocus, of the transcription factor ZENK, the retinoic acid synthesis enzyme RALDH-2, and the growth factor TGFbeta-2.<h4>Methods</h4>Chickens 11 days of age were unilaterally treated with positive or negative spectacle lenses of 7 D power. After 0, 15, 30, and 120 min, mRNA was extracted from retina and choroid, and the concentration of the mRNAs of the three candidates was measured by quantitative real time PCR in both eyes.<h4>Results</h4>ZENK in the retina and RALDH-2 in the choroid displayed parallel signs of defocus dependent changes in mRNA levels after 15 or 30 min, respectively. ZENK mRNA levels were reduced in the retina after 15 min with both types of lenses but were then up regulated at 30 min with positive lenses and down regulated with negative lenses, similar to the previously observed changes in ZENK protein levels. Changes of RALDH-2 and TGFbeta-2 mRNA levels were confined to the choroid. Treatment with negative lenses resulted in a rapid (15 min) and persistent decrease in TGFbeta-2 mRNA concentration in the choroid. Negative lenses provoked parallel but less pronounced alterations in the open fellow eyes.<h4>Conclusions</h4>Imposed defocus triggers extensive transcriptional changes of ZENK in the retina, and of TGFbeta-2 and RALHD-2 in the choroid. Changes in retina and choroid are rapid, show no phase delay with respect to each other, and can be considered, in the case of RALDH-2 and ZENK, as specific for the sign of imposed defocus. They occur prior to any morphological changes. This is consistent with a role in causing or controlling later changes in eye growth.
Addresses
  • Section of Neurobiology of the Eye, University Eye Hospital, Tuebingen, Calwerstr, Germany. perikles@uni-tuebingen.de
Autoren
  • Perikles Simon
  • Marita Feldkaemper
  • Michaela Bitzer
  • Sibylle Ohngemach
  • Frank Schaeffel
eISSN
1090-0535
Externe Identifier
  • PubMed Identifier: 15343149
Open access
false
ISSN
1090-0535
Zeitschrift
Molecular vision
Schlüsselwörter
  • Eye
  • Retina
  • Choroid
  • Animals
  • Chickens
  • Refractive Errors
  • Aldehyde Oxidoreductases
  • Transforming Growth Factor beta
  • DNA-Binding Proteins
  • Immediate-Early Proteins
  • Transcription Factors
  • RNA, Messenger
  • Reverse Transcriptase Polymerase Chain Reaction
  • Eyeglasses
  • Transcription, Genetic
  • Male
  • Retinal Dehydrogenase
  • Transforming Growth Factor beta2
Sprache
eng
Medium
Electronic
Online publication date
2004
Paginierung
588 - 597
Datum der Veröffentlichung
2004
Status
Published
Datum der Datenerfassung
2004
Titel
Early transcriptional changes of retinal and choroidal TGFbeta-2, RALDH-2, and ZENK following imposed positive and negative defocus in chickens.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
10

Datenquelle: Europe PubMed Central

Abstract
PURPOSE: Imposing defocus to the retina results in compensatory changes of axial eye growth. It is not clear which factors initially contribute to this process and whether they act on the post-translational, translational, or transcriptional level. We have measured early changes in mRNA levels, in response to imposed negative and positive defocus, of the transcription factor ZENK, the retinoic acid synthesis enzyme RALDH-2, and the growth factor TGFbeta-2. METHODS: Chickens 11 days of age were unilaterally treated with positive or negative spectacle lenses of 7 D power. After 0, 15, 30, and 120 min, mRNA was extracted from retina and choroid, and the concentration of the mRNAs of the three candidates was measured by quantitative real time PCR in both eyes. RESULTS: ZENK in the retina and RALDH-2 in the choroid displayed parallel signs of defocus dependent changes in mRNA levels after 15 or 30 min, respectively. ZENK mRNA levels were reduced in the retina after 15 min with both types of lenses but were then up regulated at 30 min with positive lenses and down regulated with negative lenses, similar to the previously observed changes in ZENK protein levels. Changes of RALDH-2 and TGFbeta-2 mRNA levels were confined to the choroid. Treatment with negative lenses resulted in a rapid (15 min) and persistent decrease in TGFbeta-2 mRNA concentration in the choroid. Negative lenses provoked parallel but less pronounced alterations in the open fellow eyes. CONCLUSIONS: Imposed defocus triggers extensive transcriptional changes of ZENK in the retina, and of TGFbeta-2 and RALHD-2 in the choroid. Changes in retina and choroid are rapid, show no phase delay with respect to each other, and can be considered, in the case of RALDH-2 and ZENK, as specific for the sign of imposed defocus. They occur prior to any morphological changes. This is consistent with a role in causing or controlling later changes in eye growth.
Autoren
  • Perikles Simon
  • Marita Feldkaemper
  • Michaela Bitzer
  • Sibylle Ohngemach
  • Frank Schaeffel
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/15343149
eISSN
1090-0535
Zeitschrift
Mol Vis
Schlüsselwörter
  • Aldehyde Oxidoreductases
  • Animals
  • Chickens
  • Choroid
  • DNA-Binding Proteins
  • Eye
  • Eyeglasses
  • Immediate-Early Proteins
  • Male
  • RNA, Messenger
  • Refractive Errors
  • Retina
  • Retinal Dehydrogenase
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription Factors
  • Transcription, Genetic
  • Transforming Growth Factor beta
  • Transforming Growth Factor beta2
Sprache
eng
Country
United States
Paginierung
588 - 597
PII
v10/a71
Datum der Veröffentlichung
2004
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2004
Titel
Early transcriptional changes of retinal and choroidal TGFbeta-2, RALDH-2, and ZENK following imposed positive and negative defocus in chickens.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
10

Datenquelle: PubMed

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