Veins are no arteries: even moderate arterial pressure induces significant adhesion molecule expression of vein grafts in an ex vivo circulation model

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • A Nolte
  • S Secker
  • T Walker
  • T-O Greiner
  • B Neumann
  • P Simon
  • G Ziemer
  • HP Wendel
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000290980600015&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: 769AE
  • PubMed Identifier: 21460776
ISSN
0021-9509
Ausgabe der Veröffentlichung
2
Zeitschrift
JOURNAL OF CARDIOVASCULAR SURGERY
Schlüsselwörter
  • Coronary artery bypass
  • Cell adhesion molecules
  • Pressure
  • Veins
Paginierung
251 - 259
Datum der Veröffentlichung
2011
Status
Published
Titel
Veins are no arteries: even moderate arterial pressure induces significant adhesion molecule expression of vein grafts in an <i>ex vivo</i> circulation model
Sub types
  • Article
Ausgabe der Zeitschrift
52

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<h4>Aim</h4>Coronary artery bypass grafting (CABG) is a standard procedure for treatment of coronary heart disease. Eighty percent of all CABGs are performed with venous grafts which then get exposed to an arterial pressure after surgery. This widely used procedure, however, is complicated by the development of alterations in the vein graft wall, leading to a decreased patency rate and graft failure. This study enlightens the influence of an even moderate arterial pressure on the gene expression of adhesion molecules in venous grafts which play a decisive role for the early induction of atherogenesis.<h4>Methods</h4>Segments of porcine vena jugularis and arteria carotis were mounted in a simulated bypass circuit and subjected to pulsatile flow. Vessel segments were examined for adhesion molecule expression with quantitative real-time - polymerase chain reaction (qRT-PCR) and adherence of leukocytes was observed by confocal laser scanning microscopy and scanning electron microscopy.<h4>Results</h4>Veins grafts subjected to an even moderate arterial pressure showed a 14-fold increase of ICAM-1 expression already after 4 hours. An arterial pressure of around 100/80 mmHg was enough to stimulate the adhesion molecule expression Furthermore it led to a 9-fold increase of leukocyte adhesion to the venous endothelium, but, in contrast this was not the case in arteries.<h4>Conclusion</h4>This study showed, that already 100 mmHg upregulates the expression of several adhesion molecules in pig veins followed by increased adhesion of leukocytes. Therefore, our data demonstrate the advantage of arteries for CABG, and that new therapeutic strategies are urgently necessary to protect vein grafts either physically or pharmacologically if arteries are not available for CABG.
Addresses
  • Department of Congenital and Pediatric Cardiac Surgery, Children Hospital, Clinical Research Laboratory, University Hospital Tuebingen, Germany.
Autoren
  • A Nolte
  • S Secker
  • T Walker
  • TO Greiner
  • B Neumann
  • P Simon
  • G Ziemer
  • HP Wendel
eISSN
1827-191X
Externe Identifier
  • PubMed Identifier: 21460776
Open access
false
ISSN
0021-9509
Ausgabe der Veröffentlichung
2
Zeitschrift
The Journal of cardiovascular surgery
Schlüsselwörter
  • Carotid Arteries
  • Jugular Veins
  • Leukocytes
  • Animals
  • Swine
  • Cell Adhesion Molecules
  • Intercellular Adhesion Molecule-1
  • E-Selectin
  • Vascular Cell Adhesion Molecule-1
  • Microscopy, Confocal
  • Microscopy, Electron, Scanning
  • Coronary Artery Bypass
  • Reverse Transcriptase Polymerase Chain Reaction
  • Perfusion
  • Cell Adhesion
  • Gene Expression Regulation
  • Blood Pressure
  • Pulsatile Flow
  • Time Factors
  • Female
  • In Vitro Techniques
Sprache
eng
Medium
Print
Paginierung
251 - 259
Datum der Veröffentlichung
2011
Status
Published
Datum der Datenerfassung
2011
Titel
Veins are no arteries: even moderate arterial pressure induces significant adhesion molecule expression of vein grafts in an ex vivo circulation model.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
52

Datenquelle: Europe PubMed Central

Abstract
AIM: Coronary artery bypass grafting (CABG) is a standard procedure for treatment of coronary heart disease. Eighty percent of all CABGs are performed with venous grafts which then get exposed to an arterial pressure after surgery. This widely used procedure, however, is complicated by the development of alterations in the vein graft wall, leading to a decreased patency rate and graft failure. This study enlightens the influence of an even moderate arterial pressure on the gene expression of adhesion molecules in venous grafts which play a decisive role for the early induction of atherogenesis. METHODS: Segments of porcine vena jugularis and arteria carotis were mounted in a simulated bypass circuit and subjected to pulsatile flow. Vessel segments were examined for adhesion molecule expression with quantitative real-time - polymerase chain reaction (qRT-PCR) and adherence of leukocytes was observed by confocal laser scanning microscopy and scanning electron microscopy. RESULTS: Veins grafts subjected to an even moderate arterial pressure showed a 14-fold increase of ICAM-1 expression already after 4 hours. An arterial pressure of around 100/80 mmHg was enough to stimulate the adhesion molecule expression Furthermore it led to a 9-fold increase of leukocyte adhesion to the venous endothelium, but, in contrast this was not the case in arteries. CONCLUSION: This study showed, that already 100 mmHg upregulates the expression of several adhesion molecules in pig veins followed by increased adhesion of leukocytes. Therefore, our data demonstrate the advantage of arteries for CABG, and that new therapeutic strategies are urgently necessary to protect vein grafts either physically or pharmacologically if arteries are not available for CABG.
Autoren
  • A Nolte
  • S Secker
  • T Walker
  • TO Greiner
  • B Neumann
  • P Simon
  • G Ziemer
  • HP Wendel
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/21460776
ISSN
0021-9509
Ausgabe der Veröffentlichung
2
Zeitschrift
J Cardiovasc Surg (Torino)
Schlüsselwörter
  • Animals
  • Blood Pressure
  • Carotid Arteries
  • Cell Adhesion
  • Cell Adhesion Molecules
  • Coronary Artery Bypass
  • E-Selectin
  • Female
  • Gene Expression Regulation
  • In Vitro Techniques
  • Intercellular Adhesion Molecule-1
  • Jugular Veins
  • Leukocytes
  • Microscopy, Confocal
  • Microscopy, Electron, Scanning
  • Perfusion
  • Pulsatile Flow
  • Reverse Transcriptase Polymerase Chain Reaction
  • Swine
  • Time Factors
  • Vascular Cell Adhesion Molecule-1
Sprache
eng
Country
Italy
Paginierung
251 - 259
PII
R37116114
Datum der Veröffentlichung
2011
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2011
Titel
Veins are no arteries: even moderate arterial pressure induces significant adhesion molecule expression of vein grafts in an ex vivo circulation model.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
52

Datenquelle: PubMed

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