Effects of positive and negative lens treatment on retinal and choroidal glucagon and glucagon receptor mRNA levels in the chicken

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • C Buck
  • F Schaeffel
  • P Simon
  • M Feldkaemper
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000188769000007&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1167/iovs.03-0789
eISSN
1552-5783
Externe Identifier
  • Clarivate Analytics Document Solution ID: 771CX
  • PubMed Identifier: 14744878
ISSN
0146-0404
Ausgabe der Veröffentlichung
2
Zeitschrift
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
Paginierung
402 - 409
Datum der Veröffentlichung
2004
Status
Published
Titel
Effects of positive and negative lens treatment on retinal and choroidal glucagon and glucagon receptor mRNA levels in the chicken
Sub types
  • Article
Ausgabe der Zeitschrift
45

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Autoren
  • Christine Buck
  • Frank Schaeffel
  • Perikles Simon
  • Marita Feldkaemper
DOI
10.1167/iovs.03-0789
ISSN
1552-5783
Ausgabe der Veröffentlichung
2
Zeitschrift
Investigative Opthalmology & Visual Science
Sprache
en
Paginierung
402 - 402
Datum der Veröffentlichung
2004
Status
Published
Herausgeber
Association for Research in Vision and Ophthalmology (ARVO)
Herausgeber URL
http://dx.doi.org/10.1167/iovs.03-0789
Datum der Datenerfassung
2017
Titel
Effects of Positive and Negative Lens Treatment on Retinal and Choroidal Glucagon and Glucagon Receptor mRNA Levels in the Chicken
Ausgabe der Zeitschrift
45

Datenquelle: Crossref

Abstract
<h4>Purpose</h4>It has been found in the chicken that the amount of retinal glucagon mRNA increases during treatment with positive lenses. Pharmacological studies support the idea that glucagon may act as a stop signal for visually induced eye growth. To gain more insight into the functional role of glucagon, the changes of glucagon and glucagon receptor mRNA concentrations in retina and choroid over time were studied. Furthermore, the abundance of glucagon and the glucagon receptor was studied in different fundal layers (retina, retinal pigment epithelium[RPE], choroid) and the blood.<h4>Methods</h4>Semiquantitative real-time RT-PCR was used to measure glucagon and glucagon receptor mRNA levels in retina and choroid after positive and negative lens treatment for 2, 6, or 24 hours, by unilateral -7- or +7-D lenses. Contralateral eyes served as the control, and completely untreated animals provided further reference data. Intravitreal colchicine injections (which are known to reduce the number of glucagon cells sharply) were used to verify that the related decline in glucagon mRNA could be measured by real-time RT-PCR.<h4>Results</h4>In the retina, treatment with -7-D lenses induced an initial upregulation of glucagon mRNA in both eyes, followed by a significant downregulation. The treatment with +7-D lenses showed a significant but transient downregulation in the control eye superimposed on a trend toward upregulation in the treated eye. However, the changes in glucagon mRNA expression were not confined to the lens-treated eyes but were also found, although sometimes to a lesser extent, in the non-lens-covered fellow eyes. There was evidence of a transient increase in glucagon receptor mRNA levels in lens-treated eyes after either -7- or +7-D lens wear. In the choroid, no effect of imposed defocus was detected. The injection of colchicine led to the destruction of approximately 75% of the glucagon amacrine cells but the mRNA level of retinal glucagon decreased by only approximately 50%. Glucagon receptor expression was found to be higher in the RPE than the retina and choroid whereas, in the blood, glucagon and glucagon receptor mRNA expression was below detection level.<h4>Conclusions</h4>The observed bidirectional regulation of glucagon mRNA in correlation with the sign of imposed defocus supports the idea that glucagon may act as a stop-and-go signal for eye growth. This is in line with a previous proposal based on studies of changes of the glucagon peptide content.
Addresses
  • Section of Neurobiology of the Eye, University Eye Hospital, Tübingen, Tübingen, Germany.
Autoren
  • Christine Buck
  • Frank Schaeffel
  • Perikles Simon
  • Marita Feldkaemper
DOI
10.1167/iovs.03-0789
eISSN
1552-5783
Externe Identifier
  • PubMed Identifier: 14744878
Open access
false
ISSN
0146-0404
Ausgabe der Veröffentlichung
2
Zeitschrift
Investigative ophthalmology & visual science
Schlüsselwörter
  • Retina
  • Choroid
  • Animals
  • Chickens
  • Refractive Errors
  • Colchicine
  • Glucagon
  • Receptors, Glucagon
  • RNA
  • RNA, Messenger
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensory Deprivation
  • Gene Expression
  • Male
Sprache
eng
Medium
Print
Paginierung
402 - 409
Datum der Veröffentlichung
2004
Status
Published
Datum der Datenerfassung
2004
Titel
Effects of positive and negative lens treatment on retinal and choroidal glucagon and glucagon receptor mRNA levels in the chicken.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
45

Datenquelle: Europe PubMed Central

Abstract
PURPOSE: It has been found in the chicken that the amount of retinal glucagon mRNA increases during treatment with positive lenses. Pharmacological studies support the idea that glucagon may act as a stop signal for visually induced eye growth. To gain more insight into the functional role of glucagon, the changes of glucagon and glucagon receptor mRNA concentrations in retina and choroid over time were studied. Furthermore, the abundance of glucagon and the glucagon receptor was studied in different fundal layers (retina, retinal pigment epithelium[RPE], choroid) and the blood. METHODS: Semiquantitative real-time RT-PCR was used to measure glucagon and glucagon receptor mRNA levels in retina and choroid after positive and negative lens treatment for 2, 6, or 24 hours, by unilateral -7- or +7-D lenses. Contralateral eyes served as the control, and completely untreated animals provided further reference data. Intravitreal colchicine injections (which are known to reduce the number of glucagon cells sharply) were used to verify that the related decline in glucagon mRNA could be measured by real-time RT-PCR. RESULTS: In the retina, treatment with -7-D lenses induced an initial upregulation of glucagon mRNA in both eyes, followed by a significant downregulation. The treatment with +7-D lenses showed a significant but transient downregulation in the control eye superimposed on a trend toward upregulation in the treated eye. However, the changes in glucagon mRNA expression were not confined to the lens-treated eyes but were also found, although sometimes to a lesser extent, in the non-lens-covered fellow eyes. There was evidence of a transient increase in glucagon receptor mRNA levels in lens-treated eyes after either -7- or +7-D lens wear. In the choroid, no effect of imposed defocus was detected. The injection of colchicine led to the destruction of approximately 75% of the glucagon amacrine cells but the mRNA level of retinal glucagon decreased by only approximately 50%. Glucagon receptor expression was found to be higher in the RPE than the retina and choroid whereas, in the blood, glucagon and glucagon receptor mRNA expression was below detection level. CONCLUSIONS: The observed bidirectional regulation of glucagon mRNA in correlation with the sign of imposed defocus supports the idea that glucagon may act as a stop-and-go signal for eye growth. This is in line with a previous proposal based on studies of changes of the glucagon peptide content.
Autoren
  • Christine Buck
  • Frank Schaeffel
  • Perikles Simon
  • Marita Feldkaemper
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/14744878
DOI
10.1167/iovs.03-0789
ISSN
0146-0404
Ausgabe der Veröffentlichung
2
Zeitschrift
Invest Ophthalmol Vis Sci
Schlüsselwörter
  • Animals
  • Chickens
  • Choroid
  • Colchicine
  • Gene Expression
  • Glucagon
  • Male
  • RNA
  • RNA, Messenger
  • Receptors, Glucagon
  • Refractive Errors
  • Retina
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensory Deprivation
Sprache
eng
Country
United States
Paginierung
402 - 409
Datum der Veröffentlichung
2004
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2004
Titel
Effects of positive and negative lens treatment on retinal and choroidal glucagon and glucagon receptor mRNA levels in the chicken.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
45

Datenquelle: PubMed

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