Inhibition of adhesion molecule expression on human venous endothelial cells by non-viral siRNA transfection

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Tobias Walker
  • Hans P Wendel
  • Liane Tetzloff
  • Claudia Raabe
  • Olaf Heidenreich
  • Perikles Simon
  • Albertus M Scheule
  • Gerhard Ziemer
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000245458200013&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1111/j.1582-4934.2007.00006.x
eISSN
1582-4934
Externe Identifier
  • Clarivate Analytics Document Solution ID: 153TN
  • PubMed Identifier: 17367508
Ausgabe der Veröffentlichung
1
Zeitschrift
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Schlüsselwörter
  • RNAi
  • venous graft disease
  • coronary bypass grafting
  • adhesion molecules
Paginierung
139 - 147
Datum der Veröffentlichung
2007
Status
Published
Titel
Inhibition of adhesion molecule expression on human venous endothelial cells by non-viral siRNA transfection
Sub types
  • Article
Ausgabe der Zeitschrift
11

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<jats:title>Abstract</jats:title><jats:p> <jats:bold>Objective:</jats:bold> Expression of adhesion molecule receptors on venous endothelial cells crucially influences the fate of venous grafts by mediating leukocyte‐endothelium interactions. These interactions include adhesion of leuko‐cytes to the endothelium, followed by transendothelial migration, leading to neointimal hyperplasia (NIH) and finally graft occlusion. Therefore, inhibition of adhesion molecule expression may be a promising strategy to improve the quality of venous grafts.We tested the efficiency of non‐viral transfection of human venous endothe‐lial cells (HVEC) with short interfering RNA (siRNA) to specifically down‐regulate adhesion molecule expression.</jats:p><jats:p> <jats:bold>Methods:</jats:bold> Primary cultures of HVEC were examined for expression of the adhesion molecules ICAM1, VCAM1 and E‐selectin (SELE) after non viral siRNA transfection. Adhesion molecule expression was measured by flow cytom‐etry, real‐time polymerase chain reaction and immunoblotting after stimulation with TNF‐α, an inflammatory cytokine.</jats:p><jats:p> <jats:bold>Results:</jats:bold> Non‐transfected cells showed a strong increase of adhesion molecule expression following cytokine stimulation (<jats:italic>P</jats:italic> &lt; 0.01). Upon transfection with specific siRNAs a sixfold decrease in ICAM1 (<jats:italic>P</jats:italic> &lt; 0.001) and SELE expression and cell positivity (<jats:italic>P</jats:italic> &lt; 0.05) and a twofold decrease in VCAM1 expression and cell positiv‐ity (<jats:italic>P</jats:italic> &lt; 0.01) Pcould be observed. SiRNA‐mediated gene suppression of adhesion molecules was also reflected by corresponding decreases in adhesion protein and transcript levels.</jats:p><jats:p> <jats:bold>Conclusions:</jats:bold> The expression of adhesion molecules on HVECs can be effectively inhibited by specific siRNAs using a safe, non‐viral transfection approach. This is a promising tool to pre‐condition venous bypass grafts in order to interfere with endothelium‐leukocyte interactions and to prohibit neointima thickening or ath‐erosclerosis, which are regarded to be the most important causes of venous graft failure.</jats:p>
Autoren
  • Tobias Walker
  • Hans P Wendel
  • Liane Tetzloff
  • Claudia Raabe
  • Olaf Heidenreich
  • Perikles Simon
  • Albertus M Scheule
  • Gerhard Ziemer
DOI
10.1111/j.1582-4934.2007.00006.x
eISSN
1582-4934
ISSN
1582-1838
Ausgabe der Veröffentlichung
1
Zeitschrift
Journal of Cellular and Molecular Medicine
Sprache
en
Online publication date
2007
Paginierung
139 - 147
Datum der Veröffentlichung
2007
Status
Published
Herausgeber
Wiley
Herausgeber URL
http://dx.doi.org/10.1111/j.1582-4934.2007.00006.x
Datum der Datenerfassung
2023
Titel
Inhibition of adhesion molecule expression on human venous endothelial cells by non‐viral siRNA transfection
Ausgabe der Zeitschrift
11

Datenquelle: Crossref

Abstract
<h4>Objective</h4>Expression of adhesion molecule receptors on venous endothelial cells crucially influences the fate of venous grafts by mediating leukocyte-endothelium interactions. These interactions include adhesion of leukocytes to the endothelium, followed by transendothelial migration, leading to neointimal hyperplasia (NIH) and finally graft occlusion. Therefore, inhibition of adhesion molecule expression may be a promising strategy to improve the quality of venous grafts. We tested the efficiency of non-viral transfection of human venous endothelial cells (HVEC) with short interfering RNA (siRNA) to specifically down-regulate adhesion molecule expression.<h4>Methods</h4>Primary cultures of HVEC were examined for expression of the adhesion molecules ICAM1, VCAM1 and E-selectin (SELE) after non viral siRNA transfection. Adhesion molecule expression was measured by flow cytometry, real-time polymerase chain reaction and immunoblotting after stimulation with TNF-alpha, an inflammatory cytokine.<h4>Results</h4>Non-transfected cells showed a strong increase of adhesion molecule expression following cytokine stimulation (P < 0.01). Upon transfection with specific siRNAs a sixfold decrease in ICAM1 (P < 0.001) and SELE expression and cell positivity (P < 0.05) and a twofold decrease in VCAM1 expression and cell positivity (P < 0.01) P could be observed. SiRNA-mediated gene suppression of adhesion molecules was also reflected by corresponding decreases in adhesion protein and transcript levels.<h4>Conclusions</h4>The expression of adhesion molecules on HVECs can be effectively inhibited by specific siRNAs using a safe, non-viral transfection approach. This is a promising tool to pre-condition venous bypass grafts in order to interfere with endothelium-leukocyte interactions and to prohibit neointima thickening or atherosclerosis, which are regarded to be the most important causes of venous graft failure.
Addresses
  • Department of Thoracic, Cardiac and Vascular Surgery, Tuebingen University Hospital, Tuebingen, Germany. tobias.walker@med.uni-tuebingen.de
Autoren
  • Tobias Walker
  • Hans P Wendel
  • Liane Tetzloff
  • Claudia Raabe
  • Olaf Heidenreich
  • Perikles Simon
  • Albertus M Scheule
  • Gerhard Ziemer
DOI
10.1111/j.1582-4934.2007.00006.x
eISSN
1582-4934
Externe Identifier
  • PubMed Identifier: 17367508
  • PubMed Central ID: PMC4401227
Open access
true
ISSN
1582-1838
Ausgabe der Veröffentlichung
1
Zeitschrift
Journal of cellular and molecular medicine
Schlüsselwörter
  • Endothelium, Vascular
  • Veins
  • Saphenous Vein
  • Cells, Cultured
  • Endothelial Cells
  • Humans
  • Fluorescein-5-isothiocyanate
  • Tumor Necrosis Factor-alpha
  • Cell Adhesion Molecules
  • Intercellular Adhesion Molecule-1
  • RNA, Small Interfering
  • Fluorescent Dyes
  • Transfection
Sprache
eng
Medium
Print
Open access status
Open Access
Paginierung
139 - 147
Datum der Veröffentlichung
2007
Status
Published
Datum der Datenerfassung
2007
Titel
Inhibition of adhesion molecule expression on human venous endothelial cells by non-viral siRNA transfection.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
11

Files

https://europepmc.org/articles/PMC4401227?pdf=render

Datenquelle: Europe PubMed Central

Abstract
OBJECTIVE: Expression of adhesion molecule receptors on venous endothelial cells crucially influences the fate of venous grafts by mediating leukocyte-endothelium interactions. These interactions include adhesion of leukocytes to the endothelium, followed by transendothelial migration, leading to neointimal hyperplasia (NIH) and finally graft occlusion. Therefore, inhibition of adhesion molecule expression may be a promising strategy to improve the quality of venous grafts. We tested the efficiency of non-viral transfection of human venous endothelial cells (HVEC) with short interfering RNA (siRNA) to specifically down-regulate adhesion molecule expression. METHODS: Primary cultures of HVEC were examined for expression of the adhesion molecules ICAM1, VCAM1 and E-selectin (SELE) after non viral siRNA transfection. Adhesion molecule expression was measured by flow cytometry, real-time polymerase chain reaction and immunoblotting after stimulation with TNF-alpha, an inflammatory cytokine. RESULTS: Non-transfected cells showed a strong increase of adhesion molecule expression following cytokine stimulation (P < 0.01). Upon transfection with specific siRNAs a sixfold decrease in ICAM1 (P < 0.001) and SELE expression and cell positivity (P < 0.05) and a twofold decrease in VCAM1 expression and cell positivity (P < 0.01) P could be observed. SiRNA-mediated gene suppression of adhesion molecules was also reflected by corresponding decreases in adhesion protein and transcript levels. CONCLUSIONS: The expression of adhesion molecules on HVECs can be effectively inhibited by specific siRNAs using a safe, non-viral transfection approach. This is a promising tool to pre-condition venous bypass grafts in order to interfere with endothelium-leukocyte interactions and to prohibit neointima thickening or atherosclerosis, which are regarded to be the most important causes of venous graft failure.
Autoren
  • Tobias Walker
  • Hans P Wendel
  • Liane Tetzloff
  • Claudia Raabe
  • Olaf Heidenreich
  • Perikles Simon
  • Albertus M Scheule
  • Gerhard Ziemer
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/17367508
DOI
10.1111/j.1582-4934.2007.00006.x
Externe Identifier
  • PubMed Central ID: PMC4401227
ISSN
1582-1838
Ausgabe der Veröffentlichung
1
Zeitschrift
J Cell Mol Med
Schlüsselwörter
  • Cell Adhesion Molecules
  • Cells, Cultured
  • Endothelial Cells
  • Endothelium, Vascular
  • Fluorescein-5-isothiocyanate
  • Fluorescent Dyes
  • Humans
  • Intercellular Adhesion Molecule-1
  • RNA, Small Interfering
  • Saphenous Vein
  • Transfection
  • Tumor Necrosis Factor-alpha
  • Veins
Sprache
eng
Country
England
Paginierung
139 - 147
PII
JCMM006
Datum der Veröffentlichung
2007
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2007
Titel
Inhibition of adhesion molecule expression on human venous endothelial cells by non-viral siRNA transfection.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
11

Datenquelle: PubMed

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