Transfection of Short-Interfering RNA Silences Adhesion Molecule Expression on Cardiac Microvascular Cells

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • T Walker
  • E Saup
  • A Nolte
  • P Simon
  • A Kornberger
  • V Steger
  • O Adolph
  • H-P Wendel
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000294662500002&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1055/s-0030-1271142
eISSN
1439-1902
Externe Identifier
  • Clarivate Analytics Document Solution ID: 817HL
  • PubMed Identifier: 21692023
ISSN
0171-6425
Ausgabe der Veröffentlichung
6
Zeitschrift
THORACIC AND CARDIOVASCULAR SURGEON
Schlüsselwörter
  • transplantation
  • heart
  • endothelium (includes related subject matter)
  • ischemia/reperfusion
Paginierung
322 - 328
Datum der Veröffentlichung
2011
Status
Published
Titel
Transfection of Short-Interfering RNA Silences Adhesion Molecule Expression on Cardiac Microvascular Cells
Sub types
  • Article
Ausgabe der Zeitschrift
59

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Autoren
  • T Walker
  • E Saup
  • A Nolte
  • P Simon
  • A Kornberger
  • V Steger
  • O Adolph
  • H-P Wendel
DOI
10.1055/s-0030-1271142
eISSN
1439-1902
ISSN
0171-6425
Ausgabe der Veröffentlichung
06
Zeitschrift
The Thoracic and Cardiovascular Surgeon
Sprache
en
Online publication date
2011
Paginierung
322 - 328
Datum der Veröffentlichung
2011
Status
Published
Herausgeber
Georg Thieme Verlag KG
Herausgeber URL
http://dx.doi.org/10.1055/s-0030-1271142
Datum der Datenerfassung
2017
Titel
Transfection of Short-Interfering RNA Silences Adhesion Molecule Expression on Cardiac Microvascular Cells
Ausgabe der Zeitschrift
59

Datenquelle: Crossref

Abstract
<h4>Background</h4>Acute rejection reactions and the development of graft arterial disease are serious limitations after transplantation. Both are connected to the expression of adhesion molecules on the activated microvascular endothelium of the allograft.<h4>Methods</h4>siRNA-mediated silencing of ESELE, ICAM-1, and VCAM-1 on human cardiac microvascular cells (HCMEC) was investigated in order to inhibit leukocyte-endothelial interactions. HCMEC were investigated for the time-dependent expression of ESELE, ICAM-1, and VCAM-1 after TNF-α stimulation and for siRNA mediated suppression using a nonviral transfecting approach. Furthermore, the effects of siRNA transfection on leukocyte binding to the endothelium were analyzed.<h4>Results</h4>Transfection with siRNA induced a significant suppression of adhesion molecule expression, regardless of whether there had been a prior single or cocktail transfection of the sequences ( P < 0.05). The quantity of attaching leukocytes was significantly reduced after an equal silencing of adhesion molecules ( P < 0.05).<h4>Conclusions</h4>This investigation demonstrates that liposomal transfection of HCMEC with specific siRNA sequences is capable of both repressing adhesion molecule expression and of reducing subsequent leukocyte-endothelial actions.
Addresses
  • Clinic for Thoracic, Cardiac and Vascular Surgery, University of Tübingen, Tübingen, Germany. tobias.walker@med.uni-tuebingen.de
Autoren
  • T Walker
  • E Saup
  • A Nolte
  • P Simon
  • A Kornberger
  • V Steger
  • O Adolph
  • H-P Wendel
DOI
10.1055/s-0030-1271142
eISSN
1439-1902
Externe Identifier
  • PubMed Identifier: 21692023
Open access
false
ISSN
0171-6425
Ausgabe der Veröffentlichung
6
Zeitschrift
The Thoracic and cardiovascular surgeon
Schlüsselwörter
  • Coronary Vessels
  • Neutrophils
  • Cells, Cultured
  • Endothelial Cells
  • Humans
  • Tumor Necrosis Factor-alpha
  • Cell Adhesion Molecules
  • Intercellular Adhesion Molecule-1
  • E-Selectin
  • Vascular Cell Adhesion Molecule-1
  • RNA, Small Interfering
  • Liposomes
  • Flow Cytometry
  • Coculture Techniques
  • Transfection
  • Cell Adhesion
  • RNA Interference
  • Time Factors
  • Microvessels
  • Real-Time Polymerase Chain Reaction
Sprache
eng
Medium
Print-Electronic
Online publication date
2011
Paginierung
322 - 328
Datum der Veröffentlichung
2011
Status
Published
Datum der Datenerfassung
2011
Titel
Transfection of short-interfering RNA silences adhesion molecule expression on cardiac microvascular cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
59

Datenquelle: Europe PubMed Central

Abstract
BACKGROUND: Acute rejection reactions and the development of graft arterial disease are serious limitations after transplantation. Both are connected to the expression of adhesion molecules on the activated microvascular endothelium of the allograft. METHODS: siRNA-mediated silencing of ESELE, ICAM-1, and VCAM-1 on human cardiac microvascular cells (HCMEC) was investigated in order to inhibit leukocyte-endothelial interactions. HCMEC were investigated for the time-dependent expression of ESELE, ICAM-1, and VCAM-1 after TNF-α stimulation and for siRNA mediated suppression using a nonviral transfecting approach. Furthermore, the effects of siRNA transfection on leukocyte binding to the endothelium were analyzed. RESULTS: Transfection with siRNA induced a significant suppression of adhesion molecule expression, regardless of whether there had been a prior single or cocktail transfection of the sequences ( P < 0.05). The quantity of attaching leukocytes was significantly reduced after an equal silencing of adhesion molecules ( P < 0.05). CONCLUSIONS: This investigation demonstrates that liposomal transfection of HCMEC with specific siRNA sequences is capable of both repressing adhesion molecule expression and of reducing subsequent leukocyte-endothelial actions.
Autoren
  • T Walker
  • E Saup
  • A Nolte
  • P Simon
  • A Kornberger
  • V Steger
  • O Adolph
  • H-P Wendel
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/21692023
DOI
10.1055/s-0030-1271142
eISSN
1439-1902
Ausgabe der Veröffentlichung
6
Zeitschrift
Thorac Cardiovasc Surg
Schlüsselwörter
  • Cell Adhesion
  • Cell Adhesion Molecules
  • Cells, Cultured
  • Coculture Techniques
  • Coronary Vessels
  • E-Selectin
  • Endothelial Cells
  • Flow Cytometry
  • Humans
  • Intercellular Adhesion Molecule-1
  • Liposomes
  • Microvessels
  • Neutrophils
  • RNA Interference
  • RNA, Small Interfering
  • Real-Time Polymerase Chain Reaction
  • Time Factors
  • Transfection
  • Tumor Necrosis Factor-alpha
  • Vascular Cell Adhesion Molecule-1
Sprache
eng
Country
Germany
Paginierung
322 - 328
Datum der Veröffentlichung
2011
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2012
Titel
Transfection of short-interfering RNA silences adhesion molecule expression on cardiac microvascular cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
59

Datenquelle: PubMed

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