Feasibility of Cell-Free DNA Measurement from the Earlobe during Physiological Exercise Testing
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Nils Haller
- Aleksandar Tomaskovic
- Thomas Stoeggl
- Perikles Simon
- Elmo Neuberger
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000816064200001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/diagnostics12061379
- eISSN
- 2075-4418
- Externe Identifier
- Clarivate Analytics Document Solution ID: 2K0VX
- PubMed Identifier: 35741187
- Ausgabe der Veröffentlichung
- 6
- Zeitschrift
- DIAGNOSTICS
- Schlüsselwörter
- cell-free DNA
- exercise physiology
- liquid biopsy
- load monitoring
- physiological exercise testing
- Artikelnummer
- ARTN 1379
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Titel
- Feasibility of Cell-Free DNA Measurement from the Earlobe during Physiological Exercise Testing
- Sub types
- Article
- Ausgabe der Zeitschrift
- 12
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:p>Circulating, cell-free DNA (cfDNA) has been discussed as an upcoming blood-based biomarker in exercise physiology, reflecting important aspects of exercise load. cfDNA blood sampling has evolved from elaborate venous to efficient capillary sampling from the fingertips. In this study, we aimed to evaluate the principal feasibility of cfDNA blood sampling from the earlobe. Therefore, we obtained cfDNA concentrations from the fingertips, earlobe, and the antecubital vein during physiological exercise testing. Significantly higher concentrations were obtained from the earlobe compared to fingertip samples. All of the measurement methods showed good to excellent repeatability (ICCs of 0.85 to 0.93). In addition, the control experiments revealed that repeated sampling from the earlobe but not from the fingertips increased cfDNA at rest. In summary, cfDNA sampling is feasible for all sampling sources. However, at rest, cfDNA collected from the earlobe tend to increase over time in the absence of physical load, potentially limiting this sampling method.</jats:p>
- Autoren
- Nils Haller
- Aleksandar Tomaskovic
- Thomas Stöggl
- Perikles Simon
- Elmo Neuberger
- DOI
- 10.3390/diagnostics12061379
- eISSN
- 2075-4418
- Ausgabe der Veröffentlichung
- 6
- Zeitschrift
- Diagnostics
- Sprache
- en
- Online publication date
- 2022
- Paginierung
- 1379 - 1379
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/diagnostics12061379
- Datum der Datenerfassung
- 2022
- Titel
- Feasibility of Cell-Free DNA Measurement from the Earlobe during Physiological Exercise Testing
- Ausgabe der Zeitschrift
- 12
Datenquelle: Crossref
- Abstract
- Circulating, cell-free DNA (cfDNA) has been discussed as an upcoming blood-based biomarker in exercise physiology, reflecting important aspects of exercise load. cfDNA blood sampling has evolved from elaborate venous to efficient capillary sampling from the fingertips. In this study, we aimed to evaluate the principal feasibility of cfDNA blood sampling from the earlobe. Therefore, we obtained cfDNA concentrations from the fingertips, earlobe, and the antecubital vein during physiological exercise testing. Significantly higher concentrations were obtained from the earlobe compared to fingertip samples. All of the measurement methods showed good to excellent repeatability (ICCs of 0.85 to 0.93). In addition, the control experiments revealed that repeated sampling from the earlobe but not from the fingertips increased cfDNA at rest. In summary, cfDNA sampling is feasible for all sampling sources. However, at rest, cfDNA collected from the earlobe tend to increase over time in the absence of physical load, potentially limiting this sampling method.
- Addresses
- Department of Sports Medicine, Rehabilitation and Disease Prevention, Johannes Gutenberg University of Mainz, 55099 Mainz, Germany.
- Autoren
- Nils Haller
- Aleksandar Tomaskovic
- Thomas Stöggl
- Perikles Simon
- Elmo Neuberger
- DOI
- 10.3390/diagnostics12061379
- eISSN
- 2075-4418
- Externe Identifier
- PubMed Identifier: 35741187
- PubMed Central ID: PMC9222055
- Open access
- true
- ISSN
- 2075-4418
- Ausgabe der Veröffentlichung
- 6
- Zeitschrift
- Diagnostics (Basel, Switzerland)
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2022
- Open access status
- Open Access
- Paginierung
- 1379
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2022
- Titel
- Feasibility of Cell-Free DNA Measurement from the Earlobe during Physiological Exercise Testing.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 12
Files
https://www.mdpi.com/2075-4418/12/6/1379/pdf?version=1654169434 https://europepmc.org/articles/PMC9222055?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Circulating, cell-free DNA (cfDNA) has been discussed as an upcoming blood-based biomarker in exercise physiology, reflecting important aspects of exercise load. cfDNA blood sampling has evolved from elaborate venous to efficient capillary sampling from the fingertips. In this study, we aimed to evaluate the principal feasibility of cfDNA blood sampling from the earlobe. Therefore, we obtained cfDNA concentrations from the fingertips, earlobe, and the antecubital vein during physiological exercise testing. Significantly higher concentrations were obtained from the earlobe compared to fingertip samples. All of the measurement methods showed good to excellent repeatability (ICCs of 0.85 to 0.93). In addition, the control experiments revealed that repeated sampling from the earlobe but not from the fingertips increased cfDNA at rest. In summary, cfDNA sampling is feasible for all sampling sources. However, at rest, cfDNA collected from the earlobe tend to increase over time in the absence of physical load, potentially limiting this sampling method.
- Date of acceptance
- 2022
- Autoren
- Nils Haller
- Aleksandar Tomaskovic
- Thomas Stöggl
- Perikles Simon
- Elmo Neuberger
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/35741187
- DOI
- 10.3390/diagnostics12061379
- Externe Identifier
- PubMed Central ID: PMC9222055
- ISSN
- 2075-4418
- Ausgabe der Veröffentlichung
- 6
- Zeitschrift
- Diagnostics (Basel)
- Schlüsselwörter
- cell-free DNA
- exercise physiology
- liquid biopsy
- load monitoring
- physiological exercise testing
- Sprache
- eng
- Country
- Switzerland
- PII
- diagnostics12061379
- Datum der Veröffentlichung
- 2022
- Status
- Published online
- Titel
- Feasibility of Cell-Free DNA Measurement from the Earlobe during Physiological Exercise Testing.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 12
Datenquelle: PubMed
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