Ablation of lysozyme M-positive cells prevents aircraft noise-induced vascular damage without improving cerebral side effects

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Abstract
<jats:title>Abstract</jats:title><jats:p>Aircraft noise induces vascular and cerebral inflammation and oxidative stress causing hypertension and cardiovascular/cerebral dysfunction. With the present studies, we sought to determine the role of myeloid cells in the vascular vs. cerebral consequences of exposure to aircraft noise. Toxin-mediated ablation of lysozyme M<jats:sup>+</jats:sup> (LysM<jats:sup>+</jats:sup>) myeloid cells was performed in LysMCre<jats:sup>iDTR</jats:sup> mice carrying a cre-inducible diphtheria toxin receptor. In the last 4d of toxin treatment, the animals were exposed to noise at maximum and mean sound pressure levels of 85 and 72 dB(A), respectively. Flow cytometry analysis revealed accumulation of CD45<jats:sup>+</jats:sup>, CD11b<jats:sup>+</jats:sup>, F4/80<jats:sup>+</jats:sup>, and Ly6G<jats:sup>−</jats:sup>Ly6C<jats:sup>+</jats:sup> cells in the aortas of noise-exposed mice, which was prevented by LysM<jats:sup>+</jats:sup> cell ablation in the periphery, whereas brain infiltrates were even exacerbated upon ablation. Aircraft noise-induced increases in blood pressure and endothelial dysfunction of the aorta and retinal/mesenteric arterioles were almost completely normalized by ablation. Correspondingly, reactive oxygen species in the aorta, heart, and retinal/mesenteric vessels were attenuated in ablated noise-exposed mice, while microglial activation and abundance in the brain was greatly increased. Expression of phagocytic NADPH oxidase (NOX-2) and vascular cell adhesion molecule-1 (VCAM-1) mRNA in the aorta was reduced, while NFκB signaling appeared to be activated in the brain upon ablation. In sum, we show dissociation of cerebral and peripheral inflammatory reactions in response to aircraft noise after LysM<jats:sup>+</jats:sup> cell ablation, wherein peripheral myeloid inflammatory cells represent a dominant part of the pathomechanism for noise stress-induced cardiovascular effects and their central nervous counterparts, microglia, as key mediators in stress responses.</jats:p>
Autoren
  • Katie Frenis
  • Johanna Helmstädter
  • Yue Ruan
  • Eva Schramm
  • Sanela Kalinovic
  • Swenja Kröller-Schön
  • Maria Teresa Bayo Jimenez
  • Omar Hahad
  • Matthias Oelze
  • Subao Jiang
  • Philip Wenzel
  • Clemens J Sommer
  • Katrin BM Frauenknecht
  • Ari Waisman
  • Adrian Gericke
  • Andreas Daiber
  • Thomas Münzel
  • Sebastian Steven
DOI
10.1007/s00395-021-00869-5
eISSN
1435-1803
ISSN
0300-8428
Ausgabe der Veröffentlichung
1
Zeitschrift
Basic Research in Cardiology
Sprache
en
Artikelnummer
31
Online publication date
2021
Datum der Veröffentlichung
2021
Status
Published
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1007/s00395-021-00869-5
Datum der Datenerfassung
2022
Titel
Ablation of lysozyme M-positive cells prevents aircraft noise-induced vascular damage without improving cerebral side effects
Ausgabe der Zeitschrift
116

Datenquelle: Crossref

Andere Metadatenquellen:
Author's licence
CC-BY
Autoren
  • Katie Frenis
  • Johanna Helmstädter
  • Yue Ruan
  • Eva Schramm
  • Sanela Kalinovic
  • Swenja Kröller-Schön
  • Maria Teresa Bayo Jimenez
  • Omar Hahad
  • Matthias Oelze
  • Subao Jiang
  • Philip Wenzel
  • Clemens J Sommer
  • Katrin BM Frauenknecht
  • Ari Waisman
  • Adrian Gericke
  • Andreas Daiber
  • Thomas Münzel
  • Sebastian Steven
Hosting institution
Universitätsbibliothek Mainz
Sammlungen
  • JGU-Publikationen
Resource version
Published version
DOI
10.1007/s00395-021-00869-5
File(s) embargoed
false
Open access
true
ISSN
1435-1803
Zeitschrift
Basic research in cardiology
Schlüsselwörter
  • 610 Medizin
  • 610 Medical sciences
Sprache
eng
Open access status
Open Access
Paginierung
31
Datum der Veröffentlichung
2021
Public URL
https://openscience.ub.uni-mainz.de/handle/20.500.12030/7519
Herausgeber
Steinkopff
Datum der Datenerfassung
2022
Datum, an dem der Datensatz öffentlich gemacht wurde
2022
Zugang
Public
Titel
Ablation of lysozyme M-positive cells prevents aircraft noise-induced vascular damage without improving cerebral side effects
Ausgabe der Zeitschrift
116

Files

ablation_of_lysozyme_mpositiv-20220801145435956.pdf

Datenquelle: OPENSCIENCE.UB

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