Ethyl Hydroxyethyl Cellulose-A Biocompatible Polymer Carrier in Blood
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Anja Eckelt
- Franziska Wichmann
- Franziska Bayer
- John Eckelt
- Jonathan Gross
- Till Opatz
- Kerstin Jurk
- Christoph Reinhardt
- Klytaimnistra Kiouptsi
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000816409700001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/ijms23126432
- eISSN
- 1422-0067
- Externe Identifier
- Clarivate Analytics Document Solution ID: 2K5XZ
- PubMed Identifier: 35742876
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Schlüsselwörter
- polymer
- nanomaterial
- ethyl hydroxyethyl cellulose
- platelets
- plasma expanders
- Artikelnummer
- ARTN 6432
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Titel
- Ethyl Hydroxyethyl Cellulose-A Biocompatible Polymer Carrier in Blood
- Sub types
- Article
- Ausgabe der Zeitschrift
- 23
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:p>The biocompatibility of carrier nanomaterials in blood is largely hampered by their activating or inhibiting role on the clotting system, which in many cases prevents safe intravascular application. Here, we characterized an aqueous colloidal ethyl hydroxyethyl cellulose (EHEC) solution and tested its effect on ex vivo clot formation, platelet aggregation, and activation by thromboelastometry, aggregometry, and flow cytometry. We compared the impact of EHEC solution on platelet aggregation with biocompatible materials used in transfusion medicine (the plasma expanders gelatin polysuccinate and hydroxyethyl starch). We demonstrate that the EHEC solution, in contrast to commercial products exhibiting Newtonian flow behavior, resembles the shear-thinning behavior of human blood. Similar to established nanomaterials that are considered biocompatible when added to blood, the EHEC exposure of resting platelets in platelet-rich plasma does not enhance tissue thromboplastin- or ellagic acid-induced blood clotting, or platelet aggregation or activation, as measured by integrin αIIbβ3 activation and P-selectin exposure. Furthermore, the addition of EHEC solution to adenosine diphosphate (ADP)-stimulated platelet-rich plasma does not affect the platelet aggregation induced by this agonist. Overall, our results suggest that EHEC may be suitable as a biocompatible carrier material in blood circulation and for applications in flow-dependent diagnostics.</jats:p>
- Autoren
- Anja Eckelt
- Franziska Wichmann
- Franziska Bayer
- John Eckelt
- Jonathan Groß
- Till Opatz
- Kerstin Jurk
- Christoph Reinhardt
- Klytaimnistra Kiouptsi
- DOI
- 10.3390/ijms23126432
- eISSN
- 1422-0067
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- International Journal of Molecular Sciences
- Sprache
- en
- Online publication date
- 2022
- Paginierung
- 6432 - 6432
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/ijms23126432
- Datum der Datenerfassung
- 2022
- Titel
- Ethyl Hydroxyethyl Cellulose—A Biocompatible Polymer Carrier in Blood
- Ausgabe der Zeitschrift
- 23
Datenquelle: Crossref
- Abstract
- The biocompatibility of carrier nanomaterials in blood is largely hampered by their activating or inhibiting role on the clotting system, which in many cases prevents safe intravascular application. Here, we characterized an aqueous colloidal ethyl hydroxyethyl cellulose (EHEC) solution and tested its effect on ex vivo clot formation, platelet aggregation, and activation by thromboelastometry, aggregometry, and flow cytometry. We compared the impact of EHEC solution on platelet aggregation with biocompatible materials used in transfusion medicine (the plasma expanders gelatin polysuccinate and hydroxyethyl starch). We demonstrate that the EHEC solution, in contrast to commercial products exhibiting Newtonian flow behavior, resembles the shear-thinning behavior of human blood. Similar to established nanomaterials that are considered biocompatible when added to blood, the EHEC exposure of resting platelets in platelet-rich plasma does not enhance tissue thromboplastin- or ellagic acid-induced blood clotting, or platelet aggregation or activation, as measured by integrin α<sub>IIb</sub>β<sub>3</sub> activation and P-selectin exposure. Furthermore, the addition of EHEC solution to adenosine diphosphate (ADP)-stimulated platelet-rich plasma does not affect the platelet aggregation induced by this agonist. Overall, our results suggest that EHEC may be suitable as a biocompatible carrier material in blood circulation and for applications in flow-dependent diagnostics.
- Addresses
- Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstrasse 1, 55131 Mainz, Germany.
- Autoren
- Anja Eckelt
- Franziska Wichmann
- Franziska Bayer
- John Eckelt
- Jonathan Groß
- Till Opatz
- Kerstin Jurk
- Christoph Reinhardt
- Klytaimnistra Kiouptsi
- DOI
- 10.3390/ijms23126432
- eISSN
- 1422-0067
- Externe Identifier
- PubMed Identifier: 35742876
- PubMed Central ID: PMC9223706
- Funding acknowledgements
- German Ministry of Economic Affairs and Energy: BMWi; 03THW13L07
- Open access
- true
- ISSN
- 1422-0067
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- International journal of molecular sciences
- Schlüsselwörter
- Blood Platelets
- Humans
- Polymers
- Cellulose
- Adenosine Diphosphate
- Platelet Function Tests
- Platelet Aggregation
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2022
- Open access status
- Open Access
- Paginierung
- 6432
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2022
- Titel
- Ethyl Hydroxyethyl Cellulose-A Biocompatible Polymer Carrier in Blood.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 23
Files
https://www.mdpi.com/1422-0067/23/12/6432/pdf?version=1654750684 https://europepmc.org/articles/PMC9223706?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- The biocompatibility of carrier nanomaterials in blood is largely hampered by their activating or inhibiting role on the clotting system, which in many cases prevents safe intravascular application. Here, we characterized an aqueous colloidal ethyl hydroxyethyl cellulose (EHEC) solution and tested its effect on ex vivo clot formation, platelet aggregation, and activation by thromboelastometry, aggregometry, and flow cytometry. We compared the impact of EHEC solution on platelet aggregation with biocompatible materials used in transfusion medicine (the plasma expanders gelatin polysuccinate and hydroxyethyl starch). We demonstrate that the EHEC solution, in contrast to commercial products exhibiting Newtonian flow behavior, resembles the shear-thinning behavior of human blood. Similar to established nanomaterials that are considered biocompatible when added to blood, the EHEC exposure of resting platelets in platelet-rich plasma does not enhance tissue thromboplastin- or ellagic acid-induced blood clotting, or platelet aggregation or activation, as measured by integrin αIIbβ3 activation and P-selectin exposure. Furthermore, the addition of EHEC solution to adenosine diphosphate (ADP)-stimulated platelet-rich plasma does not affect the platelet aggregation induced by this agonist. Overall, our results suggest that EHEC may be suitable as a biocompatible carrier material in blood circulation and for applications in flow-dependent diagnostics.
- Date of acceptance
- 2022
- Autoren
- Anja Eckelt
- Franziska Wichmann
- Franziska Bayer
- John Eckelt
- Jonathan Groß
- Till Opatz
- Kerstin Jurk
- Christoph Reinhardt
- Klytaimnistra Kiouptsi
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/35742876
- DOI
- 10.3390/ijms23126432
- eISSN
- 1422-0067
- Externe Identifier
- PubMed Central ID: PMC9223706
- Funding acknowledgements
- German Ministry of Economic Affairs and Energy: BMWi; 03THW13L07
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- Int J Mol Sci
- Schlüsselwörter
- ethyl hydroxyethyl cellulose
- nanomaterial
- plasma expanders
- platelets
- polymer
- Adenosine Diphosphate
- Blood Platelets
- Cellulose
- Humans
- Platelet Aggregation
- Platelet Function Tests
- Polymers
- Sprache
- eng
- Country
- Switzerland
- PII
- ijms23126432
- Datum der Veröffentlichung
- 2022
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Titel
- Ethyl Hydroxyethyl Cellulose-A Biocompatible Polymer Carrier in Blood.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 23
Datenquelle: PubMed
- Author's licence
- CC-BY
- Autoren
- Anja Eckelt
- Franziska Wichmann
- Franziska Bayer
- John Eckelt
- Jonathan Groß
- Till Opatz
- Kerstin Jurk
- Christoph Reinhardt
- Klytaimnistra Kiouptsi
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- DFG-491381577-G
- Resource version
- Published version
- DOI
- 10.3390/ijms23126432
- Funding acknowledgements
- Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 491381577
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 1422-0067
- Ausgabe der Veröffentlichung
- 12
- Zeitschrift
- International journal of molecular sciences
- Schlüsselwörter
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- 6432
- Datum der Veröffentlichung
- 2022
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/8583
- Herausgeber
- MDPI
- Datum der Datenerfassung
- 2023
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2023
- Zugang
- Public
- Titel
- Ethyl hydroxyethyl cellulose : a biocompatible polymer carrier in blood
- Ausgabe der Zeitschrift
- 23
Files
ethyl_hydroxyethyl_cellulosea-20230112132753512.pdf
Datenquelle: OPENSCIENCE.UB
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