Histone acetylation regulates the expression of genes involved in worker reproduction in the ant Temnothorax rugatulus

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000726070800002&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1186/s12864-021-08196-8
Externe Identifier
  • Clarivate Analytics Document Solution ID: XI4FY
  • PubMed Identifier: 34861814
ISSN
1471-2164
Ausgabe der Veröffentlichung
1
Zeitschrift
BMC GENOMICS
Schlüsselwörter
  • Gene regulation
  • epigenetics
  • phenotypic plasticity
  • reproduction
  • ants
Artikelnummer
ARTN 871
Datum der Veröffentlichung
2021
Status
Published
Titel
Histone acetylation regulates the expression of genes involved in worker reproduction in the ant <i>Temnothorax rugatulus</i>
Sub types
  • Article
Ausgabe der Zeitschrift
22

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>In insect societies, queens monopolize reproduction while workers perform tasks such as brood care or foraging. Queen loss leads to ovary development and lifespan extension in workers of many ant species. However, the underlying molecular mechanisms of this phenotypic plasticity remain unclear. Recent studies highlight the importance of epigenetics in regulating plastic traits in social insects. Thus, we investigated the role of histone acetylation in regulating worker reproduction in the ant <jats:italic>Temnothorax rugatulus</jats:italic>. We removed queens from their colonies to induce worker fecundity, and either fed workers with chemical inhibitors of histone acetylation (C646), deacetylation (TSA), or the solvent (DMSO) as control. We monitored worker number for six weeks after which we assessed ovary development and sequenced fat body mRNA.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>Workers survived better in queenless colonies. They also developed their ovaries after queen removal in control colonies as expected, but not in colonies treated with the chemical inhibitors. Both inhibitors affected gene expression, although the inhibition of histone acetylation using C646 altered the expression of more genes with immunity, fecundity, and longevity functionalities. Interestingly, these C646-treated workers shared many upregulated genes with infertile workers from queenright colonies. We also identified one gene with antioxidant properties commonly downregulated in infertile workers from queenright colonies and both C646 and TSA-treated workers from queenless colonies.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p>Our results suggest that histone acetylation is involved in the molecular regulation of worker reproduction, and thus point to an important role of histone modifications in modulating phenotypic plasticity of life history traits in social insects.</jats:p> </jats:sec>
Autoren
DOI
10.1186/s12864-021-08196-8
eISSN
1471-2164
Ausgabe der Veröffentlichung
1
Zeitschrift
BMC Genomics
Sprache
en
Artikelnummer
871
Online publication date
2021
Status
Published online
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1186/s12864-021-08196-8
Datum der Datenerfassung
2023
Titel
Histone acetylation regulates the expression of genes involved in worker reproduction in the ant Temnothorax rugatulus
Ausgabe der Zeitschrift
22

Datenquelle: Crossref

Abstract
<h4>Background</h4>In insect societies, queens monopolize reproduction while workers perform tasks such as brood care or foraging. Queen loss leads to ovary development and lifespan extension in workers of many ant species. However, the underlying molecular mechanisms of this phenotypic plasticity remain unclear. Recent studies highlight the importance of epigenetics in regulating plastic traits in social insects. Thus, we investigated the role of histone acetylation in regulating worker reproduction in the ant Temnothorax rugatulus. We removed queens from their colonies to induce worker fecundity, and either fed workers with chemical inhibitors of histone acetylation (C646), deacetylation (TSA), or the solvent (DMSO) as control. We monitored worker number for six weeks after which we assessed ovary development and sequenced fat body mRNA.<h4>Results</h4>Workers survived better in queenless colonies. They also developed their ovaries after queen removal in control colonies as expected, but not in colonies treated with the chemical inhibitors. Both inhibitors affected gene expression, although the inhibition of histone acetylation using C646 altered the expression of more genes with immunity, fecundity, and longevity functionalities. Interestingly, these C646-treated workers shared many upregulated genes with infertile workers from queenright colonies. We also identified one gene with antioxidant properties commonly downregulated in infertile workers from queenright colonies and both C646 and TSA-treated workers from queenless colonies.<h4>Conclusion</h4>Our results suggest that histone acetylation is involved in the molecular regulation of worker reproduction, and thus point to an important role of histone modifications in modulating phenotypic plasticity of life history traits in social insects.
Addresses
  • Institute of Organismic and Molecular Evolution, Johannes Gutenberg University, Mainz, Germany. mchoppin@uni-mainz.de.
Autoren
DOI
10.1186/s12864-021-08196-8
eISSN
1471-2164
Externe Identifier
  • PubMed Identifier: 34861814
  • PubMed Central ID: PMC8642982
Funding acknowledgements
  • Johannes Gutenberg-Universität Mainz:
Open access
true
ISSN
1471-2164
Ausgabe der Veröffentlichung
1
Zeitschrift
BMC genomics
Schlüsselwörter
  • Animals
  • Humans
  • Ants
  • Histones
  • Social Behavior
  • Acetylation
  • Reproduction
  • Fertility
  • Female
Sprache
eng
Medium
Electronic
Online publication date
2021
Open access status
Open Access
Paginierung
871
Datum der Veröffentlichung
2021
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2021
Titel
Histone acetylation regulates the expression of genes involved in worker reproduction in the ant Temnothorax rugatulus.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
22

Files

https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/s12864-021-08196-8 https://europepmc.org/articles/PMC8642982?pdf=render

Datenquelle: Europe PubMed Central

Abstract
BACKGROUND: In insect societies, queens monopolize reproduction while workers perform tasks such as brood care or foraging. Queen loss leads to ovary development and lifespan extension in workers of many ant species. However, the underlying molecular mechanisms of this phenotypic plasticity remain unclear. Recent studies highlight the importance of epigenetics in regulating plastic traits in social insects. Thus, we investigated the role of histone acetylation in regulating worker reproduction in the ant Temnothorax rugatulus. We removed queens from their colonies to induce worker fecundity, and either fed workers with chemical inhibitors of histone acetylation (C646), deacetylation (TSA), or the solvent (DMSO) as control. We monitored worker number for six weeks after which we assessed ovary development and sequenced fat body mRNA. RESULTS: Workers survived better in queenless colonies. They also developed their ovaries after queen removal in control colonies as expected, but not in colonies treated with the chemical inhibitors. Both inhibitors affected gene expression, although the inhibition of histone acetylation using C646 altered the expression of more genes with immunity, fecundity, and longevity functionalities. Interestingly, these C646-treated workers shared many upregulated genes with infertile workers from queenright colonies. We also identified one gene with antioxidant properties commonly downregulated in infertile workers from queenright colonies and both C646 and TSA-treated workers from queenless colonies. CONCLUSION: Our results suggest that histone acetylation is involved in the molecular regulation of worker reproduction, and thus point to an important role of histone modifications in modulating phenotypic plasticity of life history traits in social insects.
Date of acceptance
2021
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/34861814
DOI
10.1186/s12864-021-08196-8
eISSN
1471-2164
Externe Identifier
  • PubMed Central ID: PMC8642982
Ausgabe der Veröffentlichung
1
Zeitschrift
BMC Genomics
Schlüsselwörter
  • Gene regulation
  • ants
  • epigenetics
  • phenotypic plasticity
  • reproduction
  • Acetylation
  • Animals
  • Ants
  • Female
  • Fertility
  • Histones
  • Humans
  • Reproduction
  • Social Behavior
Sprache
eng
Country
England
Paginierung
871
PII
10.1186/s12864-021-08196-8
Datum der Veröffentlichung
2021
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2021
Titel
Histone acetylation regulates the expression of genes involved in worker reproduction in the ant Temnothorax rugatulus.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
22

Datenquelle: PubMed

Author's licence
CC-BY
Autoren
Hosting institution
Universitätsbibliothek Mainz
Sammlungen
  • JGU-Publikationen
Resource version
Published version
DOI
10.1186/s12864-021-08196-8
File(s) embargoed
false
Open access
true
ISSN
1471-2164
Zeitschrift
BMC genomics
Schlüsselwörter
  • 570 Biowissenschaften
  • 570 Life sciences
Sprache
eng
Open access status
Open Access
Paginierung
871
Datum der Veröffentlichung
2021
Public URL
https://openscience.ub.uni-mainz.de/handle/20.500.12030/7209
Herausgeber
BioMed Central
Datum der Datenerfassung
2022
Datum, an dem der Datensatz öffentlich gemacht wurde
2022
Zugang
Public
Titel
Histone acetylation regulates the expression of genes involved in worker reproduction in the ant Temnothorax rugatulus
Ausgabe der Zeitschrift
22

Files

histone_acetylation_regulates-20220622095333319.pdf

Datenquelle: OPENSCIENCE.UB

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