Considerations for the Analysis of Small Extracellular Vesicles in Physical Exercise
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Alexandra Brahmer
- Elmo WI Neuberger
- Perikles Simon
- Eva-Maria Kraemer-Albers
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000599296000001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3389/fphys.2020.576150
- Externe Identifier
- Clarivate Analytics Document Solution ID: PF8KH
- PubMed Identifier: 33343383
- ISSN
- 1664-042X
- Zeitschrift
- FRONTIERS IN PHYSIOLOGY
- Schlüsselwörter
- physical exercise
- extracellular vesicles
- tissue cross-talk
- circulation
- plasma
- EV isolation
- standardization
- exosomes
- Artikelnummer
- ARTN 576150
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Titel
- Considerations for the Analysis of Small Extracellular Vesicles in Physical Exercise
- Sub types
- Review
- Ausgabe der Zeitschrift
- 11
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:p>Physical exercise induces acute physiological changes leading to enhanced tissue cross-talk and a liberation of extracellular vesicles (EVs) into the circulation. EVs are cell-derived membranous entities which carry bioactive material, such as proteins and RNA species, and are important mediators of cell-cell-communication. Different types of physical exercise interventions trigger the release of diverse EV subpopulations, which are hypothesized to be involved in physiological adaptation processes leading to health benefits and longevity. Large EVs (“microvesicles” and “microparticles”) are studied frequently in the context of physical exercise using straight forward flow cytometry approaches. However, the analysis of small EVs (sEVs) including exosomes is hampered by the complex composition of blood, confounding the methodology of EV isolation and characterization. This mini review presents a concise overview of the current state of research on sEVs released upon physical exercise (ExerVs), highlighting the technical limits of ExerV analysis. The purity of EV preparations is highly influenced by the co-isolation of non-EV structures in the size range or density of EVs, such as lipoproteins and protein aggregates. Technical constraints associated with EV purification challenge the quantification of distinct ExerV populations, the identification of their cargo, and the investigation of their biological functions. Here, we offer recommendations for the isolation and characterization of ExerVs to minimize the effects of these drawbacks. Technological advances in the ExerV research field will improve understanding of the inter-cellular cross-talk induced by physical exercise leading to health benefits.</jats:p>
- Autoren
- Alexandra Brahmer
- Elmo WI Neuberger
- Perikles Simon
- Eva-Maria Krämer-Albers
- DOI
- 10.3389/fphys.2020.576150
- eISSN
- 1664-042X
- Zeitschrift
- Frontiers in Physiology
- Online publication date
- 2020
- Status
- Published online
- Herausgeber
- Frontiers Media SA
- Herausgeber URL
- http://dx.doi.org/10.3389/fphys.2020.576150
- Datum der Datenerfassung
- 2020
- Titel
- Considerations for the Analysis of Small Extracellular Vesicles in Physical Exercise
- Ausgabe der Zeitschrift
- 11
Datenquelle: Crossref
- Abstract
- Physical exercise induces acute physiological changes leading to enhanced tissue cross-talk and a liberation of extracellular vesicles (EVs) into the circulation. EVs are cell-derived membranous entities which carry bioactive material, such as proteins and RNA species, and are important mediators of cell-cell-communication. Different types of physical exercise interventions trigger the release of diverse EV subpopulations, which are hypothesized to be involved in physiological adaptation processes leading to health benefits and longevity. Large EVs ("microvesicles" and "microparticles") are studied frequently in the context of physical exercise using straight forward flow cytometry approaches. However, the analysis of small EVs (sEVs) including exosomes is hampered by the complex composition of blood, confounding the methodology of EV isolation and characterization. This mini review presents a concise overview of the current state of research on sEVs released upon physical exercise (ExerVs), highlighting the technical limits of ExerV analysis. The purity of EV preparations is highly influenced by the co-isolation of non-EV structures in the size range or density of EVs, such as lipoproteins and protein aggregates. Technical constraints associated with EV purification challenge the quantification of distinct ExerV populations, the identification of their cargo, and the investigation of their biological functions. Here, we offer recommendations for the isolation and characterization of ExerVs to minimize the effects of these drawbacks. Technological advances in the ExerV research field will improve understanding of the inter-cellular cross-talk induced by physical exercise leading to health benefits.
- Addresses
- Extracellular Vesicles Research Group, Institute of Developmental Biology and Neurobiology, Johannes Gutenberg University of Mainz, Mainz, Germany.
- Autoren
- Alexandra Brahmer
- Elmo WI Neuberger
- Perikles Simon
- Eva-Maria Krämer-Albers
- DOI
- 10.3389/fphys.2020.576150
- eISSN
- 1664-042X
- Externe Identifier
- PubMed Identifier: 33343383
- PubMed Central ID: PMC7744614
- Funding acknowledgements
- Boehringer Ingelheim Foundation:
- Open access
- true
- ISSN
- 1664-042X
- Zeitschrift
- Frontiers in physiology
- Sprache
- eng
- Medium
- Electronic-eCollection
- Online publication date
- 2020
- Open access status
- Open Access
- Paginierung
- 576150
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2020
- Titel
- Considerations for the Analysis of Small Extracellular Vesicles in Physical Exercise.
- Sub types
- review-article
- Review
- Journal Article
- Ausgabe der Zeitschrift
- 11
Files
https://www.frontiersin.org/articles/10.3389/fphys.2020.576150/pdf https://europepmc.org/articles/PMC7744614?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Physical exercise induces acute physiological changes leading to enhanced tissue cross-talk and a liberation of extracellular vesicles (EVs) into the circulation. EVs are cell-derived membranous entities which carry bioactive material, such as proteins and RNA species, and are important mediators of cell-cell-communication. Different types of physical exercise interventions trigger the release of diverse EV subpopulations, which are hypothesized to be involved in physiological adaptation processes leading to health benefits and longevity. Large EVs ("microvesicles" and "microparticles") are studied frequently in the context of physical exercise using straight forward flow cytometry approaches. However, the analysis of small EVs (sEVs) including exosomes is hampered by the complex composition of blood, confounding the methodology of EV isolation and characterization. This mini review presents a concise overview of the current state of research on sEVs released upon physical exercise (ExerVs), highlighting the technical limits of ExerV analysis. The purity of EV preparations is highly influenced by the co-isolation of non-EV structures in the size range or density of EVs, such as lipoproteins and protein aggregates. Technical constraints associated with EV purification challenge the quantification of distinct ExerV populations, the identification of their cargo, and the investigation of their biological functions. Here, we offer recommendations for the isolation and characterization of ExerVs to minimize the effects of these drawbacks. Technological advances in the ExerV research field will improve understanding of the inter-cellular cross-talk induced by physical exercise leading to health benefits.
- Date of acceptance
- 2020
- Autoren
- Alexandra Brahmer
- Elmo WI Neuberger
- Perikles Simon
- Eva-Maria Krämer-Albers
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/33343383
- DOI
- 10.3389/fphys.2020.576150
- Externe Identifier
- PubMed Central ID: PMC7744614
- ISSN
- 1664-042X
- Zeitschrift
- Front Physiol
- Schlüsselwörter
- EV isolation
- circulation
- exosomes
- extracellular vesicles
- physical exercise
- plasma
- standardization
- tissue cross-talk
- Sprache
- eng
- Country
- Switzerland
- Paginierung
- 576150
- Datum der Veröffentlichung
- 2020
- Status
- Published online
- Titel
- Considerations for the Analysis of Small Extracellular Vesicles in Physical Exercise.
- Sub types
- Journal Article
- Review
- Ausgabe der Zeitschrift
- 11
Datenquelle: PubMed
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- Eigentum von