HPMA-Based Nanocarriers for Effective Immune System Stimulation
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Stefan Kramer
- Jens Langhanki
- Matthias Krumb
- Till Opatz
- Matthias Bros
- Rudolf Zentel
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000471782900012&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1002/mabi.201800481
- eISSN
- 1616-5195
- Externe Identifier
- Clarivate Analytics Document Solution ID: ID6IX
- PubMed Identifier: 30968573
- ISSN
- 1616-5187
- Ausgabe der Veröffentlichung
- 6
- Zeitschrift
- MACROMOLECULAR BIOSCIENCE
- Schlüsselwörter
- HPMA block copolymers
- micelles
- targeting
- trimannose
- vaccines
- Artikelnummer
- ARTN 1800481
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Titel
- HPMA-Based Nanocarriers for Effective Immune System Stimulation
- Sub types
- Article
- Ausgabe der Zeitschrift
- 19
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>The selective activation of the immune system using nanoparticles as a drug delivery system is a promising field in cancer therapy. Block copolymers from HPMA and laurylmethacrylate‐<jats:italic>co</jats:italic>‐hymecromone‐methacrylate allow the preparation of multifunctionalized core‐crosslinked micelles of variable size. To activate dendritic cells (DCs) as antigen presenting cells, the carbohydrates mannose and trimannose are introduced into the hydrophilic corona as DC targeting units. To activate DCs, a lipophilic adjuvant (L18‐MDP) is incorporated into the core of the micelles. To elicit an immune response, a model antigen peptide (SIINFEKL) is attached to the polymeric nanoparticle—in addition—via a click reaction with the terminal azide. Thereafter, the differently functionalized micelles are chemically and biologically characterized. While the core‐crosslinked micelles without carbohydrate units are hardly bound by DCs, mannose and trimannose functionalization lead to a strong binding. Flow cytometric analysis and blocking studies employing mannan suggest the requirement of the mannose receptor and DC‐SIGN for effective micelle binding. It could be suppressed by blocking with mannan. Adjuvant‐loaded micelles functionalized with mannose and trimannose activate DCs, and DCs preincubated with antigen‐conjugated micelles induce proliferation of antigen‐specific CD8+ T cells.</jats:p>
- Autoren
- Stefan Kramer
- Jens Langhanki
- Matthias Krumb
- Till Opatz
- Matthias Bros
- Rudolf Zentel
- DOI
- 10.1002/mabi.201800481
- eISSN
- 1616-5195
- ISSN
- 1616-5187
- Ausgabe der Veröffentlichung
- 6
- Zeitschrift
- Macromolecular Bioscience
- Sprache
- en
- Online publication date
- 2019
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1002/mabi.201800481
- Datum der Datenerfassung
- 2023
- Titel
- HPMA‐Based Nanocarriers for Effective Immune System Stimulation
- Ausgabe der Zeitschrift
- 19
Datenquelle: Crossref
- Abstract
- The selective activation of the immune system using nanoparticles as a drug delivery system is a promising field in cancer therapy. Block copolymers from HPMA and laurylmethacrylate-co-hymecromone-methacrylate allow the preparation of multifunctionalized core-crosslinked micelles of variable size. To activate dendritic cells (DCs) as antigen presenting cells, the carbohydrates mannose and trimannose are introduced into the hydrophilic corona as DC targeting units. To activate DCs, a lipophilic adjuvant (L18-MDP) is incorporated into the core of the micelles. To elicit an immune response, a model antigen peptide (SIINFEKL) is attached to the polymeric nanoparticle-in addition-via a click reaction with the terminal azide. Thereafter, the differently functionalized micelles are chemically and biologically characterized. While the core-crosslinked micelles without carbohydrate units are hardly bound by DCs, mannose and trimannose functionalization lead to a strong binding. Flow cytometric analysis and blocking studies employing mannan suggest the requirement of the mannose receptor and DC-SIGN for effective micelle binding. It could be suppressed by blocking with mannan. Adjuvant-loaded micelles functionalized with mannose and trimannose activate DCs, and DCs preincubated with antigen-conjugated micelles induce proliferation of antigen-specific CD8+ T cells.
- Addresses
- Institute of Organic Chemistry, Johannes Gutenberg-University Mainz, Duesbergweg 10-14, ,55128, Mainz, Germany.
- Autoren
- Stefan Kramer
- Jens Langhanki
- Matthias Krumb
- Till Opatz
- Matthias Bros
- Rudolf Zentel
- DOI
- 10.1002/mabi.201800481
- eISSN
- 1616-5195
- Externe Identifier
- PubMed Identifier: 30968573
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: SFB 1066
- Open access
- false
- ISSN
- 1616-5187
- Ausgabe der Veröffentlichung
- 6
- Zeitschrift
- Macromolecular bioscience
- Schlüsselwörter
- Dendritic Cells
- Immune System
- Humans
- Azides
- Methacrylates
- Polymers
- Peptide Fragments
- Ovalbumin
- Adjuvants, Immunologic
- Drug Delivery Systems
- Micelles
- Particle Size
- Nanoparticles
- Hydrophobic and Hydrophilic Interactions
- Click Chemistry
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2019
- Paginierung
- e1800481
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Datum der Datenerfassung
- 2019
- Titel
- HPMA-Based Nanocarriers for Effective Immune System Stimulation.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 19
Datenquelle: Europe PubMed Central
- Abstract
- The selective activation of the immune system using nanoparticles as a drug delivery system is a promising field in cancer therapy. Block copolymers from HPMA and laurylmethacrylate-co-hymecromone-methacrylate allow the preparation of multifunctionalized core-crosslinked micelles of variable size. To activate dendritic cells (DCs) as antigen presenting cells, the carbohydrates mannose and trimannose are introduced into the hydrophilic corona as DC targeting units. To activate DCs, a lipophilic adjuvant (L18-MDP) is incorporated into the core of the micelles. To elicit an immune response, a model antigen peptide (SIINFEKL) is attached to the polymeric nanoparticle-in addition-via a click reaction with the terminal azide. Thereafter, the differently functionalized micelles are chemically and biologically characterized. While the core-crosslinked micelles without carbohydrate units are hardly bound by DCs, mannose and trimannose functionalization lead to a strong binding. Flow cytometric analysis and blocking studies employing mannan suggest the requirement of the mannose receptor and DC-SIGN for effective micelle binding. It could be suppressed by blocking with mannan. Adjuvant-loaded micelles functionalized with mannose and trimannose activate DCs, and DCs preincubated with antigen-conjugated micelles induce proliferation of antigen-specific CD8+ T cells.
- Autoren
- Stefan Kramer
- Jens Langhanki
- Matthias Krumb
- Till Opatz
- Matthias Bros
- Rudolf Zentel
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/30968573
- DOI
- 10.1002/mabi.201800481
- eISSN
- 1616-5195
- Ausgabe der Veröffentlichung
- 6
- Zeitschrift
- Macromol Biosci
- Schlüsselwörter
- HPMA block copolymers
- micelles
- targeting
- trimannose
- vaccines
- Adjuvants, Immunologic
- Azides
- Click Chemistry
- Dendritic Cells
- Drug Delivery Systems
- Humans
- Hydrophobic and Hydrophilic Interactions
- Immune System
- Methacrylates
- Micelles
- Nanoparticles
- Ovalbumin
- Particle Size
- Peptide Fragments
- Polymers
- Sprache
- eng
- Country
- Germany
- Paginierung
- e1800481
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Titel
- HPMA-Based Nanocarriers for Effective Immune System Stimulation.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 19
Datenquelle: PubMed
- Beziehungen:
- Eigentum von