Hepatoprotective effects of extracts, fractions and compounds from the stem bark of Pentaclethra macrophylla Benth: Evidence from in vitro and in vivo studies
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Cyrille Lionel Kamga Bomgning
- Pierre Valery Kemdoum Sinda
- Beaudelaire Kemvoufo Ponou
- Agathe Lambou Fotio
- Mathias Kenfack Tsague
- Borice Tapondjou Tsafack
- Jonas Kuehlborn
- Elvine Pami Mbuyo-Nguelefack
- Remy Bertrand Teponno
- Till Opatz
- Leon Azefack Tapondjou
- Telesphore Benoit Nguelefack
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000620859100005&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.biopha.2021.111242
- eISSN
- 1950-6007
- Externe Identifier
- Clarivate Analytics Document Solution ID: QL1RZ
- PubMed Identifier: 33486213
- ISSN
- 0753-3322
- Zeitschrift
- BIOMEDICINE & PHARMACOTHERAPY
- Schlüsselwörter
- Pentaclethra macrophylla
- Isolated compounds
- Hepatoprotection
- Structure-activity relationships
- Lipid peroxidation
- GaIN/LPS
- Artikelnummer
- ARTN 111242
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Titel
- Hepatoprotective effects of extracts, fractions and compounds from the stem bark of <i>Pentaclethra macrophylla</i> Benth: Evidence from <i>in vitro</i> and <i>in vivo</i> studies
- Sub types
- Article
- Ausgabe der Zeitschrift
- 136
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Cyrille Lionel Kamga Bomgning
- Pierre Valery Kemdoum Sinda
- Beaudelaire Kemvoufo Ponou
- Agathe Lambou Fotio
- Mathias Kenfack Tsague
- Borice Tapondjou Tsafack
- Jonas Kühlborn
- Elvine Pami Mbuyo-Nguelefack
- Rémy Bertrand Teponno
- Till Opatz
- Léon Azefack Tapondjou
- Télesphore Benoit Nguelefack
- DOI
- 10.1016/j.biopha.2021.111242
- ISSN
- 0753-3322
- Zeitschrift
- Biomedicine & Pharmacotherapy
- Sprache
- en
- Artikelnummer
- 111242
- Paginierung
- 111242 - 111242
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.biopha.2021.111242
- Datum der Datenerfassung
- 2021
- Titel
- Hepatoprotective effects of extracts, fractions and compounds from the stem bark of Pentaclethra macrophylla Benth: Evidence from in vitro and in vivo studies
- Ausgabe der Zeitschrift
- 136
Datenquelle: Crossref
- Abstract
- <h4>Aim</h4>To identify the bioactive hepatoprotective components of the ethanol extract of Pentaclethra macrophylla stem bark using in vitro and in vivo approaches.<h4>Methods</h4>The bioguided-fractionation of the ethanol extract was based on the substances' capacity to prevent in vitro, the lipid peroxidation of hepatocytes' membranes induced by hydrogen peroxide. For the in vivo hepatoprotective test, mice were treated orally with the ethyl acetate (EtOAc) fraction of the ethanol extract at doses of 50 and 75 mg/kg/day for one week and subjected to d-galactosamine/lipopolysaccharide (GaIN/LPS)-induced hepatotoxicity. Blood samples were collected for alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), TNF-α and IL-1β assays. The liver was harvested for histological and biochemical (proteins, glutathione (GSH), catalase and superoxide dismutase (SOD)) analysis.<h4>Results</h4>The ethanol extract and fractions induced concentration-dependent inhibition of lipid peroxidation (IC<sub>50</sub>: 3.21-48.90 μg/mL) greater than that of silymarin (IC<sub>50</sub>: 117.4 μg/mL). The purification of the sub-fractions of EtOAc fraction yielded: (7R)-7-hydroxyhexacosanoic acid (1), (7R)-1-(7-hydroxyhexacosanoyl) glycerol (2), bergenin (3), 11-O-galloylbergenin (4), 2-hydroxymethyl-5-(2-hydroxypropan-2-yl)phenol (5), β-sitosterol 3-O-β-d-glucopyranosyl (6) and β-sitosterol (7)), among which 11-O-galloylbergenin (IC<sub>50</sub>:1.8 μg/mL) was the most effective. The EtOAc fraction significantly reduced the serum level of ALAT, ASAT and TNF-α in vivo. This EtOAc fraction increased the liver protein content and protected the liver against structural damages, but did not boost the endogenous antioxidant parameters.<h4>Conclusion</h4>The stem bark of Pentaclethra macrophylla possesses hepatoprotective effects that may result from its capacity to inhibit lipid peroxidation and could be attributed to its active components 3, 4 and 2.
- Addresses
- Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon. Electronic address: lionel_cyrille.bozy@yahoo.fr.
- Autoren
- Cyrille Lionel Kamga Bomgning
- Pierre Valery Kemdoum Sinda
- Beaudelaire Kemvoufo Ponou
- Agathe Lambou Fotio
- Mathias Kenfack Tsague
- Borice Tapondjou Tsafack
- Jonas Kühlborn
- Elvine Pami Mbuyo-Nguelefack
- Rémy Bertrand Teponno
- Till Opatz
- Léon Azefack Tapondjou
- Télesphore Benoit Nguelefack
- DOI
- 10.1016/j.biopha.2021.111242
- eISSN
- 1950-6007
- Externe Identifier
- PubMed Identifier: 33486213
- Open access
- false
- ISSN
- 0753-3322
- Zeitschrift
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Schlüsselwörter
- Liver
- Hepatocytes
- Animals
- Mice
- Rats
- Rats, Wistar
- Fabaceae
- Plant Bark
- Plant Stems
- Disease Models, Animal
- Alanine Transaminase
- Aspartate Aminotransferases
- Tumor Necrosis Factor-alpha
- Plant Extracts
- Antioxidants
- Lipid Peroxidation
- Interleukin-1beta
- Chemical and Drug Induced Liver Injury
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2021
- Paginierung
- 111242
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Publisher licence
- CC BY-NC-ND
- Datum der Datenerfassung
- 2021
- Titel
- Hepatoprotective effects of extracts, fractions and compounds from the stem bark of Pentaclethra macrophylla Benth: Evidence from in vitro and in vivo studies.
- Sub types
- Comparative Study
- Journal Article
- Ausgabe der Zeitschrift
- 136
Datenquelle: Europe PubMed Central
- Abstract
- AIM: To identify the bioactive hepatoprotective components of the ethanol extract of Pentaclethra macrophylla stem bark using in vitro and in vivo approaches. METHODS: The bioguided-fractionation of the ethanol extract was based on the substances' capacity to prevent in vitro, the lipid peroxidation of hepatocytes' membranes induced by hydrogen peroxide. For the in vivo hepatoprotective test, mice were treated orally with the ethyl acetate (EtOAc) fraction of the ethanol extract at doses of 50 and 75 mg/kg/day for one week and subjected to d-galactosamine/lipopolysaccharide (GaIN/LPS)-induced hepatotoxicity. Blood samples were collected for alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), TNF-α and IL-1β assays. The liver was harvested for histological and biochemical (proteins, glutathione (GSH), catalase and superoxide dismutase (SOD)) analysis. RESULTS: The ethanol extract and fractions induced concentration-dependent inhibition of lipid peroxidation (IC50: 3.21-48.90 μg/mL) greater than that of silymarin (IC50: 117.4 μg/mL). The purification of the sub-fractions of EtOAc fraction yielded: (7R)-7-hydroxyhexacosanoic acid (1), (7R)-1-(7-hydroxyhexacosanoyl) glycerol (2), bergenin (3), 11-O-galloylbergenin (4), 2-hydroxymethyl-5-(2-hydroxypropan-2-yl)phenol (5), β-sitosterol 3-O-β-d-glucopyranosyl (6) and β-sitosterol (7)), among which 11-O-galloylbergenin (IC50:1.8 μg/mL) was the most effective. The EtOAc fraction significantly reduced the serum level of ALAT, ASAT and TNF-α in vivo. This EtOAc fraction increased the liver protein content and protected the liver against structural damages, but did not boost the endogenous antioxidant parameters. CONCLUSION: The stem bark of Pentaclethra macrophylla possesses hepatoprotective effects that may result from its capacity to inhibit lipid peroxidation and could be attributed to its active components 3, 4 and 2.
- Date of acceptance
- 2020
- Autoren
- Cyrille Lionel Kamga Bomgning
- Pierre Valery Kemdoum Sinda
- Beaudelaire Kemvoufo Ponou
- Agathe Lambou Fotio
- Mathias Kenfack Tsague
- Borice Tapondjou Tsafack
- Jonas Kühlborn
- Elvine Pami Mbuyo-Nguelefack
- Rémy Bertrand Teponno
- Till Opatz
- Léon Azefack Tapondjou
- Télesphore Benoit Nguelefack
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/33486213
- DOI
- 10.1016/j.biopha.2021.111242
- eISSN
- 1950-6007
- Zeitschrift
- Biomed Pharmacother
- Schlüsselwörter
- GaIN/LPS
- Hepatoprotection
- Isolated compounds
- Lipid peroxidation
- Pentaclethra macrophylla
- Structure-activity relationships
- Alanine Transaminase
- Animals
- Antioxidants
- Aspartate Aminotransferases
- Chemical and Drug Induced Liver Injury
- Disease Models, Animal
- Fabaceae
- Hepatocytes
- Interleukin-1beta
- Lipid Peroxidation
- Liver
- Mice
- Plant Bark
- Plant Extracts
- Plant Stems
- Rats, Wistar
- Tumor Necrosis Factor-alpha
- Rats
- Sprache
- eng
- Country
- France
- Paginierung
- 111242
- PII
- S0753-3322(21)00027-5
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- Hepatoprotective effects of extracts, fractions and compounds from the stem bark of Pentaclethra macrophylla Benth: Evidence from in vitro and in vivo studies.
- Sub types
- Comparative Study
- Journal Article
- Ausgabe der Zeitschrift
- 136
Datenquelle: PubMed
- Beziehungen:
- Eigentum von