Identification of the protease inhibitor miraziridine A in the Red sea sponge Theonella swinhoei

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Tanja Schirmeister
  • Paula Tabares
  • Norbert Schaschke
  • Björn Degel
  • Ute Hentschel
DOI
10.4103/0974-8490.91047
ISSN
0974-8490
Ausgabe der Veröffentlichung
1
Zeitschrift
Pharmacognosy Research
Sprache
en
Paginierung
63 - 63
Datum der Veröffentlichung
2012
Status
Published
Herausgeber
EManuscript Technologies
Herausgeber URL
http://dx.doi.org/10.4103/0974-8490.91047
Datum der Datenerfassung
2021
Titel
Identification of the protease inhibitor miraziridine A in the Red sea sponge Theonella swinhoei
Ausgabe der Zeitschrift
4

Datenquelle: Crossref

Andere Metadatenquellen:
Abstract
<h4>Background</h4>Miraziridine A, a natural peptide isolated from a marine sponge, is a potent cathepsin B inhibitor with a second-order rate constant of 1.5 × 10(4) M(-1) s(-1). In the present study, miraziridine A was isolated from the Red Sea sponge Theonella swinhoei on the basis of chromatographic and spectrometric techniques. We conclude that T. swinhoei from the Red Sea represents an alternative source of the aziridinylpeptide miraziridine A to the previously identified Theonella mirabilis from Japan. We confirmed that the metabolite is produced by marine sponges from different geographical locations.<h4>Context</h4>Marine sponges have been proven to be a rich source of secondary metabolites exhibiting a huge diversity of biological activities, including antimicrobial, antitumor and immunomodulatory activities. Theonella species (order Lithistida, Demospongiae) have been shown to be a source of anti-protease and anti-HIV secondary metabolites.<h4>Aims</h4>To identify the protease inhibitor mirazirine A in the marine sponge Theonella swinhoei.<h4>Material and methods</h4>The marine sponge Theonella swinhoei was collected by SCUBA diving in the Red Sea in Eilat (Israel). Sponge material was lyophilized and further extracted successively with cyclohexane, dichloromethane and methanol to obtain three crude extracts. LC-MS analysis was performed to confirm the presence of Miraziridine A in the dichloromethane fraction.<h4>Results</h4>In the present study, miraziridine A was isolated from the Red Sea sponge T. swinhoei on the basis of chromatographic and spectrophotometric techniques.<h4>Conclusions</h4>We conclude that T. swinhoei from the Red Sea represents an alternative source of the aziridinylpeptide miraziridine A to the previously identified Theonella mirabilis from Japan.
Addresses
  • University of Wuerzburg, Botany II, Julius-von-Sachs-Institute, Würzburg, Germany.
Autoren
  • Paula Tabares
  • Björn Degel
  • Norbert Schaschke
  • Ute Hentschel
  • Tanja Schirmeister
DOI
10.4103/0974-8490.91047
eISSN
0974-8490
Externe Identifier
  • PubMed Identifier: 22224064
  • PubMed Central ID: PMC3250042
Open access
true
ISSN
0976-4836
Ausgabe der Veröffentlichung
1
Zeitschrift
Pharmacognosy research
Sprache
eng
Medium
Print
Open access status
Open Access
Paginierung
63 - 66
Datum der Veröffentlichung
2012
Status
Published
Publisher licence
CC BY-NC-SA
Datum der Datenerfassung
2012
Titel
Identification of the protease inhibitor miraziridine A in the Red sea sponge Theonella swinhoei.
Sub types
  • other
  • Journal Article
Ausgabe der Zeitschrift
4

Datenquelle: Europe PubMed Central

Abstract
BACKGROUND: Miraziridine A, a natural peptide isolated from a marine sponge, is a potent cathepsin B inhibitor with a second-order rate constant of 1.5 × 10(4) M(-1) s(-1). In the present study, miraziridine A was isolated from the Red Sea sponge Theonella swinhoei on the basis of chromatographic and spectrometric techniques. We conclude that T. swinhoei from the Red Sea represents an alternative source of the aziridinylpeptide miraziridine A to the previously identified Theonella mirabilis from Japan. We confirmed that the metabolite is produced by marine sponges from different geographical locations. CONTEXT: Marine sponges have been proven to be a rich source of secondary metabolites exhibiting a huge diversity of biological activities, including antimicrobial, antitumor and immunomodulatory activities. Theonella species (order Lithistida, Demospongiae) have been shown to be a source of anti-protease and anti-HIV secondary metabolites. AIMS: To identify the protease inhibitor mirazirine A in the marine sponge Theonella swinhoei. MATERIAL AND METHODS: The marine sponge Theonella swinhoei was collected by SCUBA diving in the Red Sea in Eilat (Israel). Sponge material was lyophilized and further extracted successively with cyclohexane, dichloromethane and methanol to obtain three crude extracts. LC-MS analysis was performed to confirm the presence of Miraziridine A in the dichloromethane fraction. RESULTS: In the present study, miraziridine A was isolated from the Red Sea sponge T. swinhoei on the basis of chromatographic and spectrophotometric techniques. CONCLUSIONS: We conclude that T. swinhoei from the Red Sea represents an alternative source of the aziridinylpeptide miraziridine A to the previously identified Theonella mirabilis from Japan.
Date of acceptance
2011
Autoren
  • Paula Tabares
  • Björn Degel
  • Norbert Schaschke
  • Ute Hentschel
  • Tanja Schirmeister
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/22224064
DOI
10.4103/0974-8490.91047
eISSN
0974-8490
Externe Identifier
  • PubMed Central ID: PMC3250042
Ausgabe der Veröffentlichung
1
Zeitschrift
Pharmacognosy Res
Schlüsselwörter
  • Anti-protease
  • T. swinhoei
  • cathepsin L
  • miraziridine A
Sprache
eng
Country
India
Paginierung
63 - 66
PII
PR-4-63
Datum der Veröffentlichung
2012
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2012
Titel
Identification of the protease inhibitor miraziridine A in the Red sea sponge Theonella swinhoei.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
4

Datenquelle: PubMed

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