Distinct expression of potassium channels regulates visual response properties of lamina neurons in Drosophila melanogaster
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Burak Gur
- Katja Sporar
- Anne Lopez-Behling
- Marion Silies
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000519473500013&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1007/s00359-019-01385-7
- eISSN
- 1432-1351
- Externe Identifier
- Clarivate Analytics Document Solution ID: KU1MJ
- PubMed Identifier: 31823004
- ISSN
- 0340-7594
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY
- Schlüsselwörter
- Visual system
- Potassium channels
- Lamina
- Visual processing
- Drosophila
- Paginierung
- 273 - 287
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Titel
- Distinct expression of potassium channels regulates visual response properties of lamina neurons in <i>Drosophila melanogaster</i>
- Sub types
- Article
- Ausgabe der Zeitschrift
- 206
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Burak Gür
- Katja Sporar
- Anne Lopez-Behling
- Marion Silies
- DOI
- 10.1007/s00359-019-01385-7
- eISSN
- 1432-1351
- ISSN
- 0340-7594
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Journal of Comparative Physiology A
- Sprache
- en
- Online publication date
- 2019
- Paginierung
- 273 - 287
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1007/s00359-019-01385-7
- Datum der Datenerfassung
- 2022
- Titel
- Distinct expression of potassium channels regulates visual response properties of lamina neurons in Drosophila melanogaster
- Ausgabe der Zeitschrift
- 206
Datenquelle: Crossref
- Abstract
- The computational organization of sensory systems depends on the diversification of individual cell types with distinct signal-processing capabilities. The Drosophila visual system, for instance, splits information into channels with different temporal properties directly downstream of photoreceptors in the first-order interneurons of the OFF pathway, L2 and L3. However, the biophysical mechanisms that determine this specialization are largely unknown. Here, we show that the voltage-gated K<sub>a</sub> channels Shaker and Shal contribute to the response properties of the major OFF pathway input L2. L3 calcium response kinetics postsynaptic to photoreceptors resemble the sustained calcium signals of photoreceptors, whereas L2 neurons decay transiently. Based on a cell-type-specific RNA-seq data set and endogenous protein tagging, we identified Shaker and Shal as the primary candidates to shape L2 responses. Using in vivo two-photon imaging of L2 calcium signals in combination with pharmacological and genetic perturbations of these K<sub>a</sub> channels, we show that the wild-type Shaker and Shal function is to enhance L2 responses and cell-autonomously sharpen L2 kinetics. Our results reveal a role for K<sub>a</sub> channels in determining the signal-processing characteristics of a specific cell type in the visual system.
- Addresses
- Institute of Developmental Biology and Neurobiology, Johannes Gutenberg-Universität Mainz, 55128, Mainz, Germany.
- Autoren
- Burak Gür
- Katja Sporar
- Anne Lopez-Behling
- Marion Silies
- DOI
- 10.1007/s00359-019-01385-7
- eISSN
- 1432-1351
- Externe Identifier
- PubMed Identifier: 31823004
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: SI1991/1-1
- Open access
- false
- ISSN
- 0340-7594
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology
- Schlüsselwörter
- Brain
- Visual Pathways
- Interneurons
- Animals
- Animals, Genetically Modified
- Drosophila melanogaster
- Calcium Channels, L-Type
- Drosophila Proteins
- Photic Stimulation
- Visual Perception
- Calcium Signaling
- Evoked Potentials, Visual
- Kinetics
- Shaker Superfamily of Potassium Channels
- Shal Potassium Channels
- Optic Lobe, Nonmammalian
- Vision, Ocular
- Photoreceptor Cells, Invertebrate
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2019
- Paginierung
- 273 - 287
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Datum der Datenerfassung
- 2019
- Titel
- Distinct expression of potassium channels regulates visual response properties of lamina neurons in Drosophila melanogaster.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 206
Datenquelle: Europe PubMed Central
- Abstract
- The computational organization of sensory systems depends on the diversification of individual cell types with distinct signal-processing capabilities. The Drosophila visual system, for instance, splits information into channels with different temporal properties directly downstream of photoreceptors in the first-order interneurons of the OFF pathway, L2 and L3. However, the biophysical mechanisms that determine this specialization are largely unknown. Here, we show that the voltage-gated Ka channels Shaker and Shal contribute to the response properties of the major OFF pathway input L2. L3 calcium response kinetics postsynaptic to photoreceptors resemble the sustained calcium signals of photoreceptors, whereas L2 neurons decay transiently. Based on a cell-type-specific RNA-seq data set and endogenous protein tagging, we identified Shaker and Shal as the primary candidates to shape L2 responses. Using in vivo two-photon imaging of L2 calcium signals in combination with pharmacological and genetic perturbations of these Ka channels, we show that the wild-type Shaker and Shal function is to enhance L2 responses and cell-autonomously sharpen L2 kinetics. Our results reveal a role for Ka channels in determining the signal-processing characteristics of a specific cell type in the visual system.
- Date of acceptance
- 2019
- Autoren
- Burak Gür
- Katja Sporar
- Anne Lopez-Behling
- Marion Silies
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/31823004
- DOI
- 10.1007/s00359-019-01385-7
- eISSN
- 1432-1351
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- J Comp Physiol A Neuroethol Sens Neural Behav Physiol
- Schlüsselwörter
- Drosophila
- Lamina
- Potassium channels
- Visual processing
- Visual system
- Animals
- Animals, Genetically Modified
- Brain
- Calcium Channels, L-Type
- Calcium Signaling
- Drosophila Proteins
- Drosophila melanogaster
- Evoked Potentials, Visual
- Interneurons
- Kinetics
- Optic Lobe, Nonmammalian
- Photic Stimulation
- Photoreceptor Cells, Invertebrate
- Shaker Superfamily of Potassium Channels
- Shal Potassium Channels
- Vision, Ocular
- Visual Pathways
- Visual Perception
- Sprache
- eng
- Country
- Germany
- Paginierung
- 273 - 287
- PII
- 10.1007/s00359-019-01385-7
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- Distinct expression of potassium channels regulates visual response properties of lamina neurons in Drosophila melanogaster.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 206
Datenquelle: PubMed
- Beziehungen:
- Eigentum von