Kinetics and Topology of DNA Associated with Circulating Extracellular Vesicles Released during Exercise
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Elmo WI Neuberger
- Barlo Hillen
- Katharina Mayr
- Perikles Simon
- Eva-Maria Kraemer-Albers
- Alexandra Brahmer
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000643026900001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/genes12040522
- eISSN
- 2073-4425
- Externe Identifier
- Clarivate Analytics Document Solution ID: RR3UF
- PubMed Identifier: 33918465
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- GENES
- Schlüsselwörter
- extracellular vesicles
- exosomes
- cell-free DNA
- extracellular DNA
- corona
- intraluminal
- physical exercise
- vesicular genomic DNA
- human plasma
- Artikelnummer
- ARTN 522
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Titel
- Kinetics and Topology of DNA Associated with Circulating Extracellular Vesicles Released during Exercise
- Sub types
- Article
- Ausgabe der Zeitschrift
- 12
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:p>Although it is widely accepted that cancer-derived extracellular vesicles (EVs) carry DNA cargo, the association of cell-free circulating DNA (cfDNA) and EVs in plasma of healthy humans remains elusive. Using a physiological exercise model, where EVs and cfDNA are synchronously released, we aimed to characterize the kinetics and localization of DNA associated with EVs. EVs were separated from human plasma using size exclusion chromatography or immuno-affinity capture for CD9+, CD63+, and CD81+ EVs. DNA was quantified with an ultra-sensitive qPCR assay targeting repetitive LINE elements, with or without DNase digestion. This model shows that a minute part of circulating cell-free DNA is associated with EVs. During rest and following exercise, only 0.12% of the total cfDNA occurs in association with CD9+/CD63+/CD81+EVs. DNase digestion experiments indicate that the largest part of EV associated DNA is sensitive to DNase digestion and only ~20% are protected within the lumen of the separated EVs. A single bout of running or cycling exercise increases the levels of EVs, cfDNA, and EV-associated DNA. While EV surface DNA is increasing, DNAse-resistant DNA remains at resting levels, indicating that EVs released during exercise (ExerVs) do not contain DNA. Consequently, DNA is largely associated with the outer surface of circulating EVs. ExerVs recruit cfDNA to their corona, but do not carry DNA in their lumen.</jats:p>
- Autoren
- Elmo WI Neuberger
- Barlo Hillen
- Katharina Mayr
- Perikles Simon
- Eva-Maria Krämer-Albers
- Alexandra Brahmer
- DOI
- 10.3390/genes12040522
- eISSN
- 2073-4425
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Genes
- Sprache
- en
- Online publication date
- 2021
- Paginierung
- 522 - 522
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/genes12040522
- Datum der Datenerfassung
- 2021
- Titel
- Kinetics and Topology of DNA Associated with Circulating Extracellular Vesicles Released during Exercise
- Ausgabe der Zeitschrift
- 12
Datenquelle: Crossref
- Abstract
- Although it is widely accepted that cancer-derived extracellular vesicles (EVs) carry DNA cargo, the association of cell-free circulating DNA (cfDNA) and EVs in plasma of healthy humans remains elusive. Using a physiological exercise model, where EVs and cfDNA are synchronously released, we aimed to characterize the kinetics and localization of DNA associated with EVs. EVs were separated from human plasma using size exclusion chromatography or immuno-affinity capture for CD9<sup>+</sup>, CD63<sup>+</sup>, and CD81<sup>+</sup> EVs. DNA was quantified with an ultra-sensitive qPCR assay targeting repetitive LINE elements, with or without DNase digestion. This model shows that a minute part of circulating cell-free DNA is associated with EVs. During rest and following exercise, only 0.12% of the total cfDNA occurs in association with CD9<sup>+</sup>/CD63<sup>+</sup>/CD81<sup>+</sup>EVs. DNase digestion experiments indicate that the largest part of EV associated DNA is sensitive to DNase digestion and only ~20% are protected within the lumen of the separated EVs. A single bout of running or cycling exercise increases the levels of EVs, cfDNA, and EV-associated DNA. While EV surface DNA is increasing, DNAse-resistant DNA remains at resting levels, indicating that EVs released during exercise (ExerVs) do not contain DNA. Consequently, DNA is largely associated with the outer surface of circulating EVs. ExerVs recruit cfDNA to their corona, but do not carry DNA in their lumen.
- Addresses
- Department of Sports Medicine, Rehabilitation and Disease Prevention, Johannes Gutenberg University Mainz, 55099 Mainz, Germany.
- Autoren
- Elmo WI Neuberger
- Barlo Hillen
- Katharina Mayr
- Perikles Simon
- Eva-Maria Krämer-Albers
- Alexandra Brahmer
- DOI
- 10.3390/genes12040522
- eISSN
- 2073-4425
- Externe Identifier
- PubMed Identifier: 33918465
- PubMed Central ID: PMC8065814
- Open access
- true
- ISSN
- 2073-4425
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Genes
- Schlüsselwörter
- Humans
- Exercise
- Chromatography, Gel
- Polymerase Chain Reaction
- Long Interspersed Nucleotide Elements
- Kinetics
- Adult
- Female
- Male
- Young Adult
- Healthy Volunteers
- Extracellular Vesicles
- Cell-Free Nucleic Acids
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2021
- Open access status
- Open Access
- Paginierung
- 522
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2021
- Titel
- Kinetics and Topology of DNA Associated with Circulating Extracellular Vesicles Released during Exercise.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 12
Files
https://www.mdpi.com/2073-4425/12/4/522/pdf?version=1618835335 https://europepmc.org/articles/PMC8065814?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Although it is widely accepted that cancer-derived extracellular vesicles (EVs) carry DNA cargo, the association of cell-free circulating DNA (cfDNA) and EVs in plasma of healthy humans remains elusive. Using a physiological exercise model, where EVs and cfDNA are synchronously released, we aimed to characterize the kinetics and localization of DNA associated with EVs. EVs were separated from human plasma using size exclusion chromatography or immuno-affinity capture for CD9+, CD63+, and CD81+ EVs. DNA was quantified with an ultra-sensitive qPCR assay targeting repetitive LINE elements, with or without DNase digestion. This model shows that a minute part of circulating cell-free DNA is associated with EVs. During rest and following exercise, only 0.12% of the total cfDNA occurs in association with CD9+/CD63+/CD81+EVs. DNase digestion experiments indicate that the largest part of EV associated DNA is sensitive to DNase digestion and only ~20% are protected within the lumen of the separated EVs. A single bout of running or cycling exercise increases the levels of EVs, cfDNA, and EV-associated DNA. While EV surface DNA is increasing, DNAse-resistant DNA remains at resting levels, indicating that EVs released during exercise (ExerVs) do not contain DNA. Consequently, DNA is largely associated with the outer surface of circulating EVs. ExerVs recruit cfDNA to their corona, but do not carry DNA in their lumen.
- Date of acceptance
- 2021
- Autoren
- Elmo WI Neuberger
- Barlo Hillen
- Katharina Mayr
- Perikles Simon
- Eva-Maria Krämer-Albers
- Alexandra Brahmer
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/33918465
- DOI
- 10.3390/genes12040522
- eISSN
- 2073-4425
- Externe Identifier
- PubMed Central ID: PMC8065814
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Genes (Basel)
- Schlüsselwörter
- cell-free DNA
- corona
- exosomes
- extracellular DNA
- extracellular vesicles
- human plasma
- intraluminal
- physical exercise
- vesicular genomic DNA
- Adult
- Cell-Free Nucleic Acids
- Chromatography, Gel
- Exercise
- Extracellular Vesicles
- Female
- Healthy Volunteers
- Humans
- Kinetics
- Long Interspersed Nucleotide Elements
- Male
- Polymerase Chain Reaction
- Young Adult
- Sprache
- eng
- Country
- Switzerland
- PII
- genes12040522
- Datum der Veröffentlichung
- 2021
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- Kinetics and Topology of DNA Associated with Circulating Extracellular Vesicles Released during Exercise.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 12
Datenquelle: PubMed
- Author's licence
- CC-BY
- Autoren
- Elmo WI Neuberger
- Barlo Hillen
- Katharina Mayr
- Perikles Simon
- Eva-Maria Krämer-Albers
- Alexandra Brahmer
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- JGU-Publikationen
- Resource version
- Published version
- DOI
- 10.3390/genes12040522
- Funding acknowledgements
- Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainz
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 2073-4425
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Genes
- Schlüsselwörter
- 570 Biowissenschaften
- 570 Life sciences
- 796 Sport
- 796 Athletic and outdoor sports and games
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- 522
- Datum der Veröffentlichung
- 2021
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/6503
- Herausgeber
- MDPI
- Herausgeber URL
- https://doi.org/10.3390/genes12040522
- Datum der Datenerfassung
- 2021
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Zugang
- Public
- Titel
- Kinetics and topology of DNA associated with circulating extracellular vesicles released during exercise
- Ausgabe der Zeitschrift
- 12
Files
neuberger_elmo-kinetics_and_t-20211112120608184.pdf
Datenquelle: OPENSCIENCE.UB
- Beziehungen: