Resolving Binding Events on the [OPDT1] Multifunctional Human Serum Albumin
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Lea Wenskowsky
- Michael Wagner
- Johannes Reusch
- Herman Schreuder
- Hans Matter
- Till Opatz
- Stefan Matthias Petry
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000520526500001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1002/cmdc.202000069
- eISSN
- 1860-7187
- Externe Identifier
- Clarivate Analytics Document Solution ID: LJ8EM
- PubMed Identifier: 32162429
- ISSN
- 1860-7179
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- CHEMMEDCHEM
- Schlüsselwörter
- multicolor assays
- switchSENSE technology
- kinetics investigations
- drug interactions
- albumin binding
- Paginierung
- 738 - 743
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Titel
- Resolving Binding Events on the [OPDT1] Multifunctional Human Serum Albumin
- Sub types
- Article
- Ausgabe der Zeitschrift
- 15
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Physiological processes rely on initial recognition events between cellular components and other molecules or modalities. Biomolecules can have multiple sites or mode of interaction with other molecular entities, so that a resolution of the individual binding events in terms of spatial localization as well as association and dissociation kinetics is required for a meaningful description. Here we describe a trichromatic fluorescent binding‐ and displacement assay for simultaneous monitoring of three individual binding sites in the important transporter and binding protein human serum albumin. Independent investigations of binding events by X‐ray crystallography and time‐resolved dynamics measurements (switchSENSE technology) confirm the validity of the assay, the localization of binding sites and furthermore reveal conformational changes associated with ligand binding. The described assay system allows for the detailed characterization of albumin‐binding drugs and is therefore well‐suited for prediction of drug‐drug and drug‐food interactions. Moreover, conformational changes, usually associated with binding events, can also be analyzed.</jats:p>
- Autoren
- Lea Wenskowsky
- Michael Wagner
- Johannes Reusch
- Herman Schreuder
- Hans Matter
- Till Opatz
- Stefan Matthias Petry
- DOI
- 10.1002/cmdc.202000069
- eISSN
- 1860-7187
- ISSN
- 1860-7179
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- ChemMedChem
- Sprache
- en
- Online publication date
- 2020
- Paginierung
- 738 - 743
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1002/cmdc.202000069
- Datum der Datenerfassung
- 2023
- Titel
- Resolving Binding Events on the Multifunctional Human Serum Albumin
- Ausgabe der Zeitschrift
- 15
Datenquelle: Crossref
- Abstract
- Physiological processes rely on initial recognition events between cellular components and other molecules or modalities. Biomolecules can have multiple sites or mode of interaction with other molecular entities, so that a resolution of the individual binding events in terms of spatial localization as well as association and dissociation kinetics is required for a meaningful description. Here we describe a trichromatic fluorescent binding- and displacement assay for simultaneous monitoring of three individual binding sites in the important transporter and binding protein human serum albumin. Independent investigations of binding events by X-ray crystallography and time-resolved dynamics measurements (switchSENSE technology) confirm the validity of the assay, the localization of binding sites and furthermore reveal conformational changes associated with ligand binding. The described assay system allows for the detailed characterization of albumin-binding drugs and is therefore well-suited for prediction of drug-drug and drug-food interactions. Moreover, conformational changes, usually associated with binding events, can also be analyzed.
- Addresses
- Institute of Organic Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, 55128, Mainz, Germany.
- Autoren
- Lea Wenskowsky
- Michael Wagner
- Johannes Reusch
- Herman Schreuder
- Hans Matter
- Till Opatz
- Stefan Matthias Petry
- DOI
- 10.1002/cmdc.202000069
- eISSN
- 1860-7187
- Externe Identifier
- PubMed Identifier: 32162429
- PubMed Central ID: PMC7318646
- Open access
- true
- ISSN
- 1860-7179
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- ChemMedChem
- Schlüsselwörter
- Humans
- Boron Compounds
- Ibuprofen
- 4-Chloro-7-nitrobenzofurazan
- Warfarin
- Lauric Acids
- Crystallography, X-Ray
- Binding Sites
- Molecular Structure
- Fluorescence
- Molecular Dynamics Simulation
- Serum Albumin, Human
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2020
- Open access status
- Open Access
- Paginierung
- 738 - 743
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Publisher licence
- CC BY-NC-ND
- Datum der Datenerfassung
- 2020
- Titel
- Resolving Binding Events on the Multifunctional Human Serum Albumin.
- Sub types
- brief-report
- Journal Article
- Ausgabe der Zeitschrift
- 15
Files
https://europepmc.org/articles/PMC7318646?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Physiological processes rely on initial recognition events between cellular components and other molecules or modalities. Biomolecules can have multiple sites or mode of interaction with other molecular entities, so that a resolution of the individual binding events in terms of spatial localization as well as association and dissociation kinetics is required for a meaningful description. Here we describe a trichromatic fluorescent binding- and displacement assay for simultaneous monitoring of three individual binding sites in the important transporter and binding protein human serum albumin. Independent investigations of binding events by X-ray crystallography and time-resolved dynamics measurements (switchSENSE technology) confirm the validity of the assay, the localization of binding sites and furthermore reveal conformational changes associated with ligand binding. The described assay system allows for the detailed characterization of albumin-binding drugs and is therefore well-suited for prediction of drug-drug and drug-food interactions. Moreover, conformational changes, usually associated with binding events, can also be analyzed.
- Autoren
- Lea Wenskowsky
- Michael Wagner
- Johannes Reusch
- Herman Schreuder
- Hans Matter
- Till Opatz
- Stefan Matthias Petry
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/32162429
- DOI
- 10.1002/cmdc.202000069
- eISSN
- 1860-7187
- Externe Identifier
- PubMed Central ID: PMC7318646
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- ChemMedChem
- Schlüsselwörter
- albumin binding
- drug interactions
- kinetics investigations
- multicolor assays
- switchSENSE technology
- 4-Chloro-7-nitrobenzofurazan
- Binding Sites
- Boron Compounds
- Crystallography, X-Ray
- Fluorescence
- Humans
- Ibuprofen
- Lauric Acids
- Molecular Dynamics Simulation
- Molecular Structure
- Serum Albumin, Human
- Warfarin
- Sprache
- eng
- Country
- Germany
- Paginierung
- 738 - 743
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- Resolving Binding Events on the Multifunctional Human Serum Albumin.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 15
Datenquelle: PubMed
- Author's licence
- CC-BY-NC-ND
- Autoren
- Lea Wenskowsky
- Michael Wagner
- Johannes Reusch
- Herman Schreuder
- Hans Matter
- Till Opatz
- Stefan Matthias Petry
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- JGU-Publikationen
- Resource version
- Published version
- DOI
- 10.1002/cmdc.202000069
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 1860-7187
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- ChemMedChem
- Schlüsselwörter
- 540 Chemie
- 540 Chemistry and allied sciences
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- 738 - 743
- Datum der Veröffentlichung
- 2020
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/6266
- Herausgeber
- Wiley-VCH
- Herausgeber URL
- https://doi.org/10.1002/cmdc.202000069
- Datum der Datenerfassung
- 2021
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Zugang
- Public
- Titel
- Resolving binding events on the multifunctional human serum albumin
- Ausgabe der Zeitschrift
- 15
Files
dr._wenskowsky_lea-resolving_bind-20210804150339253.pdf
Datenquelle: OPENSCIENCE.UB
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