Pseudogenes as an alternative source of natural antisense transcripts
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Enrique M Muro
- Miguel A Andrade-Navarro
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000285340100002&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1186/1471-2148-10-338
- Externe Identifier
- Clarivate Analytics Document Solution ID: 694ZJ
- PubMed Identifier: 21047404
- ISSN
- 1471-2148
- Zeitschrift
- BMC EVOLUTIONARY BIOLOGY
- Artikelnummer
- ARTN 338
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Titel
- Pseudogenes as an alternative source of natural antisense transcripts
- Sub types
- Article
- Ausgabe der Zeitschrift
- 10
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Enrique M Muro
- Miguel A Andrade-Navarro
- DOI
- 10.1186/1471-2148-10-338
- ISSN
- 1471-2148
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC Evolutionary Biology
- Sprache
- en
- Paginierung
- 338 - 338
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1186/1471-2148-10-338
- Datum der Datenerfassung
- 2016
- Titel
- Pseudogenes as an alternative source of natural antisense transcripts
- Ausgabe der Zeitschrift
- 10
Datenquelle: Crossref
- Abstract
- <h4>Background</h4>Naturally occurring antisense transcripts (NATs) are non-coding RNAs that may regulate the activity of sense transcripts to which they bind because of complementarity. NATs that are not located in the gene they regulate (trans-NATs) have better chances to evolve than cis-NATs, which is evident when the sense strand of the cis-NAT is part of a protein coding gene. However, the generation of a trans-NAT requires the formation of a relatively large region of complementarity to the gene it regulates.<h4>Results</h4>Pseudogene formation may be one evolutionary mechanism that generates trans-NATs to the parental gene. For example, this could occur if the parental gene is regulated by a cis-NAT that is copied as a trans-NAT in the pseudogene. To support this we identified human pseudogenes with a trans-NAT to the parental gene in their antisense strand by analysis of the database of expressed sequence tags (ESTs). We found that the mutations that appeared in these trans-NATs after the pseudogene formation do not show the flat distribution that would be expected in a non functional transcript. Instead, we found higher similarity to the parental gene in a region nearby the 3' end of the trans-NATs.<h4>Conclusions</h4>Our results do not imply a functional relation of the trans-NAT arising from pseudogenes over their respective parental genes but add evidence for it and stress the importance of duplication mechanisms of genetic material in the generation of non-coding RNAs. We also provide a plausible explanation for the large transcripts that can be found in the antisense strand of some pseudogenes.
- Addresses
- Max-Delbrück Center for Molecular Medicine, Robert Rössle Str, 10, 13125 Berlin, Germany. enrique.muro@mdc-berlin.de
- Autoren
- Enrique M Muro
- Miguel A Andrade-Navarro
- DOI
- 10.1186/1471-2148-10-338
- eISSN
- 1471-2148
- Externe Identifier
- PubMed Identifier: 21047404
- PubMed Central ID: PMC2984423
- Open access
- true
- ISSN
- 1471-2148
- Zeitschrift
- BMC evolutionary biology
- Schlüsselwörter
- Animals
- Humans
- RNA, Antisense
- Evolution, Molecular
- Pseudogenes
- Expressed Sequence Tags
- Databases, Genetic
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2010
- Open access status
- Open Access
- Paginierung
- 338
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2010
- Titel
- Pseudogenes as an alternative source of natural antisense transcripts.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 10
Files
https://bmcecolevol.biomedcentral.com/counter/pdf/10.1186/1471-2148-10-338 http://www.biomedcentral.com/content/pdf/1471-2148-10-338.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21047404/pdf/?tool=EBI https://europepmc.org/articles/PMC2984423?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- BACKGROUND: Naturally occurring antisense transcripts (NATs) are non-coding RNAs that may regulate the activity of sense transcripts to which they bind because of complementarity. NATs that are not located in the gene they regulate (trans-NATs) have better chances to evolve than cis-NATs, which is evident when the sense strand of the cis-NAT is part of a protein coding gene. However, the generation of a trans-NAT requires the formation of a relatively large region of complementarity to the gene it regulates. RESULTS: Pseudogene formation may be one evolutionary mechanism that generates trans-NATs to the parental gene. For example, this could occur if the parental gene is regulated by a cis-NAT that is copied as a trans-NAT in the pseudogene. To support this we identified human pseudogenes with a trans-NAT to the parental gene in their antisense strand by analysis of the database of expressed sequence tags (ESTs). We found that the mutations that appeared in these trans-NATs after the pseudogene formation do not show the flat distribution that would be expected in a non functional transcript. Instead, we found higher similarity to the parental gene in a region nearby the 3' end of the trans-NATs. CONCLUSIONS: Our results do not imply a functional relation of the trans-NAT arising from pseudogenes over their respective parental genes but add evidence for it and stress the importance of duplication mechanisms of genetic material in the generation of non-coding RNAs. We also provide a plausible explanation for the large transcripts that can be found in the antisense strand of some pseudogenes.
- Date of acceptance
- 2010
- Autoren
- Enrique M Muro
- Miguel A Andrade-Navarro
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/21047404
- DOI
- 10.1186/1471-2148-10-338
- eISSN
- 1471-2148
- Externe Identifier
- PubMed Central ID: PMC2984423
- Zeitschrift
- BMC Evol Biol
- Schlüsselwörter
- Animals
- Databases, Genetic
- Evolution, Molecular
- Expressed Sequence Tags
- Humans
- Pseudogenes
- RNA, Antisense
- Sprache
- eng
- Country
- England
- Paginierung
- 338
- PII
- 1471-2148-10-338
- Datum der Veröffentlichung
- 2010
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2010
- Titel
- Pseudogenes as an alternative source of natural antisense transcripts.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 10
Datenquelle: PubMed
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