Unravelling the biosynthesis of pyriculol in the rice blast fungus Magnaporthe oryzae
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Stefan Jacob
- Thomas Groetsch
- Andrew J Foster
- Anja Schueffler
- Patrick H Rieger
- Louis P Sandjo
- Johannes C Liermann
- Till Opatz
- Eckhard Thines
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000400277400012&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1099/mic.0.000396
- eISSN
- 1465-2080
- Externe Identifier
- Clarivate Analytics Document Solution ID: ET4TH
- PubMed Identifier: 27902426
- ISSN
- 1350-0872
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- MICROBIOLOGY-SGM
- Schlüsselwörter
- pyriculol
- phytotoxin
- Magnaporthe oryzae
- secondary metabolite
- PKS gene cluster
- secondary metabolite biosynthesis
- gene cluster regulation
- virulence
- polyketide synthases
- plant-microbe interaction
- Paginierung
- 541 - 553
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Titel
- Unravelling the biosynthesis of pyriculol in the rice blast fungus <i>Magnaporthe oryzae</i>
- Sub types
- Article
- Ausgabe der Zeitschrift
- 163
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Stefan Jacob
- Thomas Grötsch
- Andrew J Foster
- Anja Schüffler
- Patrick H Rieger
- Louis P Sandjo
- Johannes C Liermann
- Till Opatz
- Eckhard Thines
- DOI
- 10.1099/mic.0.000396
- eISSN
- 1465-2080
- ISSN
- 1350-0872
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Microbiology
- Sprache
- en
- Paginierung
- 541 - 553
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Herausgeber
- Microbiology Society
- Herausgeber URL
- http://dx.doi.org/10.1099/mic.0.000396
- Datum der Datenerfassung
- 2019
- Titel
- Unravelling the biosynthesis of pyriculol in the rice blast fungus Magnaporthe oryzae
- Ausgabe der Zeitschrift
- 163
Datenquelle: Crossref
- Abstract
- Pyriculol was isolated from the rice blast fungus Magnaporthe oryzae and found to induce lesion formation on rice leaves. These findings suggest that it could be involved in virulence. The gene MoPKS19 was identified to encode a polyketide synthase essential for the production of the polyketide pyriculol in the rice blast fungus M. oryzae. The transcript abundance of MoPKS19 correlates with the biosynthesis rate of pyriculol in a time-dependent manner. Furthermore, gene inactivation of MoPKS19 resulted in a mutant unable to produce pyriculol, pyriculariol and their dihydro derivatives. Inactivation of a putative oxidase-encoding gene MoC19OXR1, which was found to be located in the genome close to MoPKS19, resulted in a mutant exclusively producing dihydropyriculol and dihydropyriculariol. By contrast, overexpression of MoC19OXR1 resulted in a mutant strain only producing pyriculol. The MoPKS19 cluster, furthermore, comprises two transcription factors MoC19TRF1 and MoC19TRF2, which were both found individually to act as negative regulators repressing gene expression of MoPKS19. Additionally, extracts of ΔMopks19 and ΔMoC19oxr1 made from axenic cultures failed to induce lesions on rice leaves compared to extracts of the wild-type strain. Consequently, pyriculol and its isomer pyriculariol appear to be the only lesion-inducing secondary metabolites produced by M. oryzae wild-type (MoWT) under these culture conditions. Interestingly, the mutants unable to produce pyriculol and pyriculariol were as pathogenic as MoWT, demonstrating that pyriculol is not required for infection.
- Addresses
- Institut für Biotechnologie und Wirkstoff-Forschung gGmbH (IBWF), Erwin-Schrödinger Str. 56, D-67663 Kaiserslautern, Germany.
- Autoren
- Stefan Jacob
- Thomas Grötsch
- Andrew J Foster
- Anja Schüffler
- Patrick H Rieger
- Louis P Sandjo
- Johannes C Liermann
- Till Opatz
- Eckhard Thines
- DOI
- 10.1099/mic.0.000396
- eISSN
- 1465-2080
- Externe Identifier
- PubMed Identifier: 27902426
- PubMed Central ID: PMC5817273
- Open access
- false
- ISSN
- 1350-0872
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Microbiology (Reading, England)
- Schlüsselwörter
- Magnaporthe
- Plant Leaves
- Fatty Alcohols
- Benzaldehydes
- Polyketide Synthases
- Transcription Factors
- Plant Diseases
- Gene Expression Regulation, Fungal
- Multigene Family
- Polyketides
- Secondary Metabolism
- Oryza
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2017
- Paginierung
- 541 - 553
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Datum der Datenerfassung
- 2016
- Titel
- Unravelling the biosynthesis of pyriculol in the rice blast fungus Magnaporthe oryzae.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 163
Files
https://europepmc.org/articles/PMC5817273?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Pyriculol was isolated from the rice blast fungus Magnaporthe oryzae and found to induce lesion formation on rice leaves. These findings suggest that it could be involved in virulence. The gene MoPKS19 was identified to encode a polyketide synthase essential for the production of the polyketide pyriculol in the rice blast fungus M. oryzae. The transcript abundance of MoPKS19 correlates with the biosynthesis rate of pyriculol in a time-dependent manner. Furthermore, gene inactivation of MoPKS19 resulted in a mutant unable to produce pyriculol, pyriculariol and their dihydro derivatives. Inactivation of a putative oxidase-encoding gene MoC19OXR1, which was found to be located in the genome close to MoPKS19, resulted in a mutant exclusively producing dihydropyriculol and dihydropyriculariol. By contrast, overexpression of MoC19OXR1 resulted in a mutant strain only producing pyriculol. The MoPKS19 cluster, furthermore, comprises two transcription factors MoC19TRF1 and MoC19TRF2, which were both found individually to act as negative regulators repressing gene expression of MoPKS19. Additionally, extracts of ΔMopks19 and ΔMoC19oxr1 made from axenic cultures failed to induce lesions on rice leaves compared to extracts of the wild-type strain. Consequently, pyriculol and its isomer pyriculariol appear to be the only lesion-inducing secondary metabolites produced by M. oryzae wild-type (MoWT) under these culture conditions. Interestingly, the mutants unable to produce pyriculol and pyriculariol were as pathogenic as MoWT, demonstrating that pyriculol is not required for infection.
- Autoren
- Stefan Jacob
- Thomas Grötsch
- Andrew J Foster
- Anja Schüffler
- Patrick H Rieger
- Louis P Sandjo
- Johannes C Liermann
- Till Opatz
- Eckhard Thines
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/27902426
- DOI
- 10.1099/mic.0.000396
- eISSN
- 1465-2080
- Externe Identifier
- PubMed Central ID: PMC5817273
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Microbiology (Reading)
- Schlüsselwörter
- Benzaldehydes
- Fatty Alcohols
- Gene Expression Regulation, Fungal
- Magnaporthe
- Multigene Family
- Oryza
- Plant Diseases
- Plant Leaves
- Polyketide Synthases
- Polyketides
- Secondary Metabolism
- Transcription Factors
- Sprache
- eng
- Country
- England
- Paginierung
- 541 - 553
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2017
- Titel
- Unravelling the biosynthesis of pyriculol in the rice blast fungus Magnaporthe oryzae.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 163
Datenquelle: PubMed
- Beziehungen:
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