In-Depth Immune-Oncology Studies of the Tumor Microenvironment in a Humanized Melanoma Mouse Model
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Jonathan Schupp
- Arne Christians
- Niklas Zimmer
- Lukas Gleue
- Helmut Jonuleit
- Mark Helm
- Andrea Tuettenberg
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000615289200001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/ijms22031011
- eISSN
- 1422-0067
- Externe Identifier
- Clarivate Analytics Document Solution ID: QD1LO
- PubMed Identifier: 33498319
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Schlüsselwörter
- Chipcytometry
- multiplex immunohistochemistry
- melanoma
- humanized mice
- tumor microenvironment
- flow cytometry
- immunohistochemistry
- Artikelnummer
- ARTN 1011
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Titel
- In-Depth Immune-Oncology Studies of the Tumor Microenvironment in a Humanized Melanoma Mouse Model
- Sub types
- Article
- Ausgabe der Zeitschrift
- 22
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:p>The presence and interaction of immune cells in the tumor microenvironment is of significant importance and has a great impact on disease progression and response to therapy. Hence, their identification is of high interest for prognosis and treatment decisions. Besides detailed phenotypic analyses of immune, as well as tumor cells, spatial analyses is an important parameter in the complex interplay of neoplastic and immune cells—especially when moving into focus efforts to develop and validate new therapeutic strategies. Ex vivo analysis of tumor samples by immunohistochemistry staining methods conserves spatial information is restricted to single markers, while flow cytometry (disrupting tissue into single cell suspensions) provides access to markers in larger numbers. Nevertheless, this comes at the cost of scarifying morphological information regarding tissue localization and cell–cell contacts. Further detrimental effects incurred by, for example, tissue digestion include staining artifacts. Consequently, ongoing efforts are directed towards methods that preserve, completely or in part, spatial information, while increasing the number of markers that can potentially be interrogated to the level of conventional flow cytometric methods. Progression in multiplex immunohistochemistry in the last ten years overcame the limitation to 1–2 markers in classical staining methods using DAB with counter stains or even pure chemical staining methods. In this study, we compared the multiplex method Chipcytometry to flow cytometry and classical IHC-P using DAB and hematoxylin. Chipcytometry uses frozen or paraffin-embedded tissue sections stained with readily available commercial fluorophore-labeled antibodies in repetitive cycles of staining and bleaching. The iterative staining approach enables sequential analysis of a virtually unlimited number of markers on the same sample, thereby identifying immune cell subpopulations in the tumor microenvironment in the present study in a humanized mouse melanoma model.</jats:p>
- Autoren
- Jonathan Schupp
- Arne Christians
- Niklas Zimmer
- Lukas Gleue
- Helmut Jonuleit
- Mark Helm
- Andrea Tuettenberg
- DOI
- 10.3390/ijms22031011
- eISSN
- 1422-0067
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- International Journal of Molecular Sciences
- Sprache
- en
- Online publication date
- 2021
- Paginierung
- 1011 - 1011
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/ijms22031011
- Datum der Datenerfassung
- 2021
- Titel
- In-Depth Immune-Oncology Studies of the Tumor Microenvironment in a Humanized Melanoma Mouse Model
- Ausgabe der Zeitschrift
- 22
Datenquelle: Crossref
- Abstract
- The presence and interaction of immune cells in the tumor microenvironment is of significant importance and has a great impact on disease progression and response to therapy. Hence, their identification is of high interest for prognosis and treatment decisions. Besides detailed phenotypic analyses of immune, as well as tumor cells, spatial analyses is an important parameter in the complex interplay of neoplastic and immune cells-especially when moving into focus efforts to develop and validate new therapeutic strategies. Ex vivo analysis of tumor samples by immunohistochemistry staining methods conserves spatial information is restricted to single markers, while flow cytometry (disrupting tissue into single cell suspensions) provides access to markers in larger numbers. Nevertheless, this comes at the cost of scarifying morphological information regarding tissue localization and cell-cell contacts. Further detrimental effects incurred by, for example, tissue digestion include staining artifacts. Consequently, ongoing efforts are directed towards methods that preserve, completely or in part, spatial information, while increasing the number of markers that can potentially be interrogated to the level of conventional flow cytometric methods. Progression in multiplex immunohistochemistry in the last ten years overcame the limitation to 1-2 markers in classical staining methods using DAB with counter stains or even pure chemical staining methods. In this study, we compared the multiplex method Chipcytometry to flow cytometry and classical IHCP using DAB and hematoxylin. Chipcytometry uses frozen or paraffin-embedded tissue sections stained with readily available commercial fluorophore-labeled antibodies in repetitive cycles of staining and bleaching. The iterative staining approach enables sequential analysis of a virtually unlimited number of markers on the same sample, thereby identifying immune cell subpopulations in the tumor microenvironment in the present study in a humanized mouse melanoma model.
- Addresses
- Department of Dermatology, University Medical Center of the Johannes Gutenberg University Mainz, 55131 Mainz, Germany.
- Autoren
- Jonathan Schupp
- Arne Christians
- Niklas Zimmer
- Lukas Gleue
- Helmut Jonuleit
- Mark Helm
- Andrea Tuettenberg
- DOI
- 10.3390/ijms22031011
- eISSN
- 1422-0067
- Externe Identifier
- PubMed Identifier: 33498319
- PubMed Central ID: PMC7864015
- Funding acknowledgements
- Wilhelm-Sander-Stiftung: 2017.143.1
- Deutsche Forschungsgemeinschaft: CRC1066 TP B3
- Open access
- true
- ISSN
- 1422-0067
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- International journal of molecular sciences
- Schlüsselwörter
- Cells, Cultured
- Cell Line, Tumor
- Animals
- Humans
- Mice
- Melanoma
- HLA-A2 Antigen
- Flow Cytometry
- Immunohistochemistry
- Immunophenotyping
- Transgenes
- Middle Aged
- Female
- Tumor Microenvironment
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2021
- Open access status
- Open Access
- Paginierung
- 1011
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2021
- Titel
- In-Depth Immune-Oncology Studies of the Tumor Microenvironment in a Humanized Melanoma Mouse Model.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 22
Files
https://www.mdpi.com/1422-0067/22/3/1011/pdf https://europepmc.org/articles/PMC7864015?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- The presence and interaction of immune cells in the tumor microenvironment is of significant importance and has a great impact on disease progression and response to therapy. Hence, their identification is of high interest for prognosis and treatment decisions. Besides detailed phenotypic analyses of immune, as well as tumor cells, spatial analyses is an important parameter in the complex interplay of neoplastic and immune cells-especially when moving into focus efforts to develop and validate new therapeutic strategies. Ex vivo analysis of tumor samples by immunohistochemistry staining methods conserves spatial information is restricted to single markers, while flow cytometry (disrupting tissue into single cell suspensions) provides access to markers in larger numbers. Nevertheless, this comes at the cost of scarifying morphological information regarding tissue localization and cell-cell contacts. Further detrimental effects incurred by, for example, tissue digestion include staining artifacts. Consequently, ongoing efforts are directed towards methods that preserve, completely or in part, spatial information, while increasing the number of markers that can potentially be interrogated to the level of conventional flow cytometric methods. Progression in multiplex immunohistochemistry in the last ten years overcame the limitation to 1-2 markers in classical staining methods using DAB with counter stains or even pure chemical staining methods. In this study, we compared the multiplex method Chipcytometry to flow cytometry and classical IHCP using DAB and hematoxylin. Chipcytometry uses frozen or paraffin-embedded tissue sections stained with readily available commercial fluorophore-labeled antibodies in repetitive cycles of staining and bleaching. The iterative staining approach enables sequential analysis of a virtually unlimited number of markers on the same sample, thereby identifying immune cell subpopulations in the tumor microenvironment in the present study in a humanized mouse melanoma model.
- Date of acceptance
- 2021
- Autoren
- Jonathan Schupp
- Arne Christians
- Niklas Zimmer
- Lukas Gleue
- Helmut Jonuleit
- Mark Helm
- Andrea Tuettenberg
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/33498319
- DOI
- 10.3390/ijms22031011
- eISSN
- 1422-0067
- Externe Identifier
- PubMed Central ID: PMC7864015
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: CRC1066 TP B3
- Wilhelm-Sander-Stiftung: 2017.143.1
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Int J Mol Sci
- Schlüsselwörter
- Chipcytometry
- flow cytometry
- humanized mice
- immunohistochemistry
- melanoma
- multiplex immunohistochemistry
- tumor microenvironment
- Animals
- Cell Line, Tumor
- Cells, Cultured
- Female
- Flow Cytometry
- HLA-A2 Antigen
- Humans
- Immunohistochemistry
- Immunophenotyping
- Melanoma
- Mice
- Middle Aged
- Transgenes
- Tumor Microenvironment
- Sprache
- eng
- Country
- Switzerland
- PII
- ijms22031011
- Datum der Veröffentlichung
- 2021
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- In-Depth Immune-Oncology Studies of the Tumor Microenvironment in a Humanized Melanoma Mouse Model.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 22
Datenquelle: PubMed
- Author's licence
- CC-BY
- Autoren
- Jonathan Schupp
- Arne Christians
- Niklas Zimmer
- Lukas Gleue
- Helmut Jonuleit
- Mark Helm
- Andrea Tüttenberg
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- JGU-Publikationen
- Resource version
- Published version
- DOI
- 10.3390/ijms22031011
- Funding acknowledgements
- Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainz
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 1422-0067
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- International journal of molecular sciences
- Schlüsselwörter
- 540 Chemie
- 540 Chemistry and allied sciences
- 570 Biowissenschaften
- 570 Life sciences
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- 1011
- Datum der Veröffentlichung
- 2021
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/5616
- Herausgeber
- Molecular Diversity Preservation International
- Herausgeber URL
- https://doi.org/10.3390/ijms22031011
- Datum der Datenerfassung
- 2021
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Zugang
- Public
- Titel
- In-depth immune-oncology studies of the tumor microenvironment in a humanized melanoma mouse model
- Ausgabe der Zeitschrift
- 22
Files
schupp_jonathan-in-depth_immun-20210129160031853.pdf
Datenquelle: OPENSCIENCE.UB
- Author's licence
- InCopyright
- Autoren
- Jonathan Schupp
- Arne Christians
- Niklas Zimmer
- Lukas Gleue
- Helmut Jonuleit
- Mark Helm
- Andrea Tüttenberg
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- JGU-Publikationen
- Resource version
- Published version
- DOI
- 10.25358/openscience-5923
- Funding acknowledgements
- Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainz
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 1422-0067
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- International journal of molecular sciences
- Schlüsselwörter
- 570 Biowissenschaften
- 570 Life sciences
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Online publication date
- 2021
- Paginierung
- 1011
- Datum der Veröffentlichung
- 2021
- Herausgeber
- Molecular Diversity Preservation International
- Publisher licence
- CC BY
- Herausgeber URL
- https://doi.org/10.3390/ijms22031011
- Datum der Datenerfassung
- 2021
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Zugang
- Deleted
- Titel
- In-depth immune-oncology studies of the tumor microenvironment in a humanized melanoma mouse model
- Ausgabe der Zeitschrift
- 22
Files
schupp_jonathan-in-depth_immun-20210514135141430.pdf
Datenquelle: OPENSCIENCE.UB
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