Metaproteomic analysis of atmospheric aerosol samples
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Fobang Liu
- Senchao Lai
- Kathrin Reinmuth-Selzle
- Jan Frederik Scheel
- Janine Froehlich-Nowoisky
- Viviane R Despres
- Thorsten Hoffmann
- Ulrich Poeschl
- Christopher J Kampf
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000382681900011&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1007/s00216-016-9747-x
- eISSN
- 1618-2650
- Externe Identifier
- Clarivate Analytics Document Solution ID: DV1LF
- PubMed Identifier: 27411545
- ISSN
- 1618-2642
- Ausgabe der Veröffentlichung
- 23
- Zeitschrift
- ANALYTICAL AND BIOANALYTICAL CHEMISTRY
- Schlüsselwörter
- Metaproteomics
- Atmospheric aerosols
- Bioanalytical methods
- HPLC
- Mass spectrometry
- Paginierung
- 6337 - 6348
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Titel
- Metaproteomic analysis of atmospheric aerosol samples
- Sub types
- Article
- Ausgabe der Zeitschrift
- 408
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Fobang Liu
- Senchao Lai
- Kathrin Reinmuth-Selzle
- Jan Frederik Scheel
- Janine Fröhlich-Nowoisky
- Viviane R Després
- Thorsten Hoffmann
- Ulrich Pöschl
- Christopher J Kampf
- DOI
- 10.1007/s00216-016-9747-x
- eISSN
- 1618-2650
- ISSN
- 1618-2642
- Ausgabe der Veröffentlichung
- 23
- Zeitschrift
- Analytical and Bioanalytical Chemistry
- Sprache
- en
- Online publication date
- 2016
- Paginierung
- 6337 - 6348
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1007/s00216-016-9747-x
- Datum der Datenerfassung
- 2017
- Titel
- Metaproteomic analysis of atmospheric aerosol samples
- Ausgabe der Zeitschrift
- 408
Datenquelle: Crossref
- Abstract
- Metaproteomic analysis of air particulate matter provides information about the abundance and properties of bioaerosols in the atmosphere and their influence on climate and public health. We developed and applied efficient methods for the extraction and analysis of proteins from glass fiber filter samples of total, coarse, and fine particulate matter. Size exclusion chromatography was applied to remove matrix components, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied for protein fractionation according to molecular size, followed by in-gel digestion and LC-MS/MS analysis of peptides using a hybrid Quadrupole-Orbitrap MS. Maxquant software and the Swiss-Prot database were used for protein identification. In samples collected at a suburban location in central Europe, we found proteins that originated mainly from plants, fungi, and bacteria, which constitute a major fraction of primary biological aerosol particles (PBAP) in the atmosphere. Allergenic proteins were found in coarse and fine particle samples, and indications for atmospheric degradation of proteins were observed. Graphical abstract Workflow for the metaproteomic analysis of atmospheric aerosol samples.
- Addresses
- Department of Multiphase Chemistry, Max Planck Institute for Chemistry, Hahn-Meitner-Weg 1, 55128, Mainz, Germany.
- Autoren
- Fobang Liu
- Senchao Lai
- Kathrin Reinmuth-Selzle
- Jan Frederik Scheel
- Janine Fröhlich-Nowoisky
- Viviane R Després
- Thorsten Hoffmann
- Ulrich Pöschl
- Christopher J Kampf
- DOI
- 10.1007/s00216-016-9747-x
- eISSN
- 1618-2650
- Externe Identifier
- PubMed Identifier: 27411545
- PubMed Central ID: PMC5009178
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: KA 4008/1-1
- China Scholarship Council:
- Open access
- true
- ISSN
- 1618-2642
- Ausgabe der Veröffentlichung
- 23
- Zeitschrift
- Analytical and bioanalytical chemistry
- Schlüsselwörter
- Proteins
- Bacterial Proteins
- Fungal Proteins
- Plant Proteins
- Allergens
- Aerosols
- Air Pollutants
- Chromatography, High Pressure Liquid
- Electrophoresis, Polyacrylamide Gel
- Proteomics
- Atmosphere
- Databases, Protein
- Particulate Matter
- Tandem Mass Spectrometry
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2016
- Open access status
- Open Access
- Paginierung
- 6337 - 6348
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2016
- Titel
- Metaproteomic analysis of atmospheric aerosol samples.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 408
Files
https://link.springer.com/content/pdf/10.1007%2Fs00216-016-9747-x.pdf https://europepmc.org/articles/PMC5009178?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Metaproteomic analysis of air particulate matter provides information about the abundance and properties of bioaerosols in the atmosphere and their influence on climate and public health. We developed and applied efficient methods for the extraction and analysis of proteins from glass fiber filter samples of total, coarse, and fine particulate matter. Size exclusion chromatography was applied to remove matrix components, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied for protein fractionation according to molecular size, followed by in-gel digestion and LC-MS/MS analysis of peptides using a hybrid Quadrupole-Orbitrap MS. Maxquant software and the Swiss-Prot database were used for protein identification. In samples collected at a suburban location in central Europe, we found proteins that originated mainly from plants, fungi, and bacteria, which constitute a major fraction of primary biological aerosol particles (PBAP) in the atmosphere. Allergenic proteins were found in coarse and fine particle samples, and indications for atmospheric degradation of proteins were observed. Graphical abstract Workflow for the metaproteomic analysis of atmospheric aerosol samples.
- Date of acceptance
- 2016
- Autoren
- Fobang Liu
- Senchao Lai
- Kathrin Reinmuth-Selzle
- Jan Frederik Scheel
- Janine Fröhlich-Nowoisky
- Viviane R Després
- Thorsten Hoffmann
- Ulrich Pöschl
- Christopher J Kampf
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/27411545
- DOI
- 10.1007/s00216-016-9747-x
- eISSN
- 1618-2650
- Externe Identifier
- PubMed Central ID: PMC5009178
- Ausgabe der Veröffentlichung
- 23
- Zeitschrift
- Anal Bioanal Chem
- Schlüsselwörter
- Atmospheric aerosols
- Bioanalytical methods
- HPLC
- Mass spectrometry
- Metaproteomics
- Aerosols
- Air Pollutants
- Allergens
- Atmosphere
- Bacterial Proteins
- Chromatography, High Pressure Liquid
- Databases, Protein
- Electrophoresis, Polyacrylamide Gel
- Fungal Proteins
- Particulate Matter
- Plant Proteins
- Proteins
- Proteomics
- Tandem Mass Spectrometry
- Sprache
- eng
- Country
- Germany
- Paginierung
- 6337 - 6348
- PII
- 10.1007/s00216-016-9747-x
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2018
- Titel
- Metaproteomic analysis of atmospheric aerosol samples.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 408
Datenquelle: PubMed
- Beziehungen:
- Eigentum von