Anti-inflammatory effects of cinnamon extract and identification of active compounds influencing the TLR2 and TLR4 signaling pathways

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Anne Schink
  • Katerina Naumoska
  • Zoran Kitanovski
  • Christopher Johannes Kampf
  • Janine Froehlich-Nowoisky
  • Eckhard Thines
  • Ulrich Poeschl
  • Detlef Schuppan
  • Kurt Lucas
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000450437000042&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1039/c8fo01286e
eISSN
2042-650X
Externe Identifier
  • Clarivate Analytics Document Solution ID: HA7BX
  • PubMed Identifier: 30379176
ISSN
2042-6496
Ausgabe der Veröffentlichung
11
Zeitschrift
FOOD & FUNCTION
Paginierung
5950 - 5964
Datum der Veröffentlichung
2018
Status
Published
Titel
Anti-inflammatory effects of cinnamon extract and identification of active compounds influencing the TLR2 and TLR4 signaling pathways
Sub types
  • Article
Ausgabe der Zeitschrift
9

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<p>Cinnamon extract and its active compounds attenuate TLR2-/TLR4-mediated inflammatory processes.</p>
Autoren
  • Anne Schink
  • Katerina Naumoska
  • Zoran Kitanovski
  • Christopher Johannes Kampf
  • Janine Fröhlich-Nowoisky
  • Eckhard Thines
  • Ulrich Pöschl
  • Detlef Schuppan
  • Kurt Lucas
DOI
10.1039/c8fo01286e
eISSN
2042-650X
ISSN
2042-6496
Ausgabe der Veröffentlichung
11
Zeitschrift
Food &amp; Function
Sprache
en
Online publication date
2018
Paginierung
5950 - 5964
Status
Published online
Herausgeber
Royal Society of Chemistry (RSC)
Herausgeber URL
http://dx.doi.org/10.1039/c8fo01286e
Datum der Datenerfassung
2024
Titel
Anti-inflammatory effects of cinnamon extract and identification of active compounds influencing the TLR2 and TLR4 signaling pathways
Ausgabe der Zeitschrift
9

Datenquelle: Crossref

Abstract
<h4>Purpose</h4>Inflammatory processes are involved in many diseases. The bark of Cinnamomum verum and its extracts are well known for anti-inflammatory effects, but the underlying active compounds and chemical mechanisms are not yet fully identified. The objective of this study was to elucidate how cinnamon extract, specifically active compounds, and their combinations influence the signaling pathways of inflammation, especially through toll-like receptors TLR2 and TLR4.<h4>Methods</h4>Bioassay-guided fractionation was performed for standard ethanolic cinnamon extract using high performance liquid chromatography followed by compound identification in the determined active fractions by high-resolution mass spectrometry and gas chromatography-mass spectrometry. THP-1 monocytes were pre-incubated with cinnamon extract, cinnamon fractions or its compounds and stimulated with lipopolysaccharides (LPS), followed by determination of interleukin 8 (IL-8) secretion, and phosphorylation of protein kinase B (Akt), nuclear factor (NF)-κB inhibitor alpha (IκBα) and p38. Furthermore, testing was performed in stimulated HEK-TLR2 and HEK-TLR4 reporter cells for direct receptor agonistic effects.<h4>Results</h4>Among the identified compounds, trans-cinnamaldehyde and p-cymene significantly reduced the LPS-dependent IL-8 secretion in THP-1 monocytes. Synergistic anti-inflammatory effects were observed for combinations of trans-cinnamaldehyde with p-cymene, cinnamyl alcohol or cinnamic acid. Moreover, cinnamon extract as well as trans-cinnamaldehyde and p-cymene mitigated the phosphorylation of Akt and IκBα.<h4>Conclusions</h4>Trans-cinnamaldehyde and p-cymene contribute to the strong anti-inflammatory effects of cinnamon extract. Furthermore, our experiments indicate that also synergistic effects among compounds that do not exhibit anti-inflammatory effects themselves might be present to positively influence the beneficial effects of cinnamon bark extract.
Addresses
  • Multiphase Chemistry Department, Max Planck Institute for Chemistry, Hahn-Meitner-Weg 1, 55128 Mainz, Germany. k.lucas@mpic.de.
Autoren
  • Anne Schink
  • Katerina Naumoska
  • Zoran Kitanovski
  • Christopher Johannes Kampf
  • Janine Fröhlich-Nowoisky
  • Eckhard Thines
  • Ulrich Pöschl
  • Detlef Schuppan
  • Kurt Lucas
DOI
10.1039/c8fo01286e
eISSN
2042-650X
Externe Identifier
  • PubMed Identifier: 30379176
Funding acknowledgements
  • Deutscher Akademischer Austauschdienst: 57366807
  • Javna Agencija za Raziskovalno Dejavnost RS: BI-DE-17-19-14
  • Johannes Gutenberg-Universität Mainz:
Open access
false
ISSN
2042-6496
Ausgabe der Veröffentlichung
11
Zeitschrift
Food & function
Schlüsselwörter
  • Monocytes
  • Humans
  • Cinnamomum zeylanicum
  • Acrolein
  • Monoterpenes
  • Lipopolysaccharides
  • NF-kappa B
  • Anti-Inflammatory Agents
  • Interleukin-8
  • Plant Extracts
  • Signal Transduction
  • Cell Survival
  • Phosphorylation
  • Drug Synergism
  • Proto-Oncogene Proteins c-akt
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • NF-KappaB Inhibitor alpha
  • THP-1 Cells
  • Cymenes
Sprache
eng
Medium
Print
Paginierung
5950 - 5964
Datum der Veröffentlichung
2018
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2018
Titel
Anti-inflammatory effects of cinnamon extract and identification of active compounds influencing the TLR2 and TLR4 signaling pathways.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
9

Datenquelle: Europe PubMed Central

Abstract
PURPOSE: Inflammatory processes are involved in many diseases. The bark of Cinnamomum verum and its extracts are well known for anti-inflammatory effects, but the underlying active compounds and chemical mechanisms are not yet fully identified. The objective of this study was to elucidate how cinnamon extract, specifically active compounds, and their combinations influence the signaling pathways of inflammation, especially through toll-like receptors TLR2 and TLR4. METHODS: Bioassay-guided fractionation was performed for standard ethanolic cinnamon extract using high performance liquid chromatography followed by compound identification in the determined active fractions by high-resolution mass spectrometry and gas chromatography-mass spectrometry. THP-1 monocytes were pre-incubated with cinnamon extract, cinnamon fractions or its compounds and stimulated with lipopolysaccharides (LPS), followed by determination of interleukin 8 (IL-8) secretion, and phosphorylation of protein kinase B (Akt), nuclear factor (NF)-κB inhibitor alpha (IκBα) and p38. Furthermore, testing was performed in stimulated HEK-TLR2 and HEK-TLR4 reporter cells for direct receptor agonistic effects. RESULTS: Among the identified compounds, trans-cinnamaldehyde and p-cymene significantly reduced the LPS-dependent IL-8 secretion in THP-1 monocytes. Synergistic anti-inflammatory effects were observed for combinations of trans-cinnamaldehyde with p-cymene, cinnamyl alcohol or cinnamic acid. Moreover, cinnamon extract as well as trans-cinnamaldehyde and p-cymene mitigated the phosphorylation of Akt and IκBα. CONCLUSIONS: Trans-cinnamaldehyde and p-cymene contribute to the strong anti-inflammatory effects of cinnamon extract. Furthermore, our experiments indicate that also synergistic effects among compounds that do not exhibit anti-inflammatory effects themselves might be present to positively influence the beneficial effects of cinnamon bark extract.
Autoren
  • Anne Schink
  • Katerina Naumoska
  • Zoran Kitanovski
  • Christopher Johannes Kampf
  • Janine Fröhlich-Nowoisky
  • Eckhard Thines
  • Ulrich Pöschl
  • Detlef Schuppan
  • Kurt Lucas
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/30379176
DOI
10.1039/c8fo01286e
eISSN
2042-650X
Ausgabe der Veröffentlichung
11
Zeitschrift
Food Funct
Schlüsselwörter
  • Acrolein
  • Anti-Inflammatory Agents
  • Cell Survival
  • Cinnamomum zeylanicum
  • Cymenes
  • Drug Synergism
  • Humans
  • Interleukin-8
  • Lipopolysaccharides
  • Monocytes
  • Monoterpenes
  • NF-KappaB Inhibitor alpha
  • NF-kappa B
  • Phosphorylation
  • Plant Extracts
  • Proto-Oncogene Proteins c-akt
  • Signal Transduction
  • THP-1 Cells
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
Sprache
eng
Country
England
Paginierung
5950 - 5964
Datum der Veröffentlichung
2018
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2019
Titel
Anti-inflammatory effects of cinnamon extract and identification of active compounds influencing the TLR2 and TLR4 signaling pathways.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
9

Datenquelle: PubMed

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