Real-Time Nuclear Magnetic Resonance Detection of Fumarase Activity Using Parahydrogen-Hyperpolarized [1-13C]Fumarate

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • James Eills
  • Eleonora Cavallari
  • Carla Carrera
  • Dmitry Budker
  • Silvio Aime
  • Francesca Reineri
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000505627300038&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1021/jacs.9b10094
eISSN
1520-5126
Externe Identifier
  • Clarivate Analytics Document Solution ID: KA2KV
  • PubMed Identifier: 31762271
ISSN
0002-7863
Ausgabe der Veröffentlichung
51
Zeitschrift
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Paginierung
20209 - 20214
Datum der Veröffentlichung
2019
Status
Published
Titel
Real-Time Nuclear Magnetic Resonance Detection of Fumarase Activity Using Parahydrogen-Hyperpolarized [1-<SUP>13</SUP>C]Fumarate
Sub types
  • Article
Ausgabe der Zeitschrift
141

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Autoren
  • James Eills
  • Eleonora Cavallari
  • Carla Carrera
  • Dmitry Budker
  • Silvio Aime
  • Francesca Reineri
DOI
10.1021/jacs.9b10094
eISSN
1520-5126
ISSN
0002-7863
Ausgabe der Veröffentlichung
51
Zeitschrift
Journal of the American Chemical Society
Sprache
en
Online publication date
2019
Paginierung
20209 - 20214
Datum der Veröffentlichung
2019
Status
Published
Herausgeber
American Chemical Society (ACS)
Herausgeber URL
http://dx.doi.org/10.1021/jacs.9b10094
Datum der Datenerfassung
2023
Titel
Real-Time Nuclear Magnetic Resonance Detection of Fumarase Activity Using Parahydrogen-Hyperpolarized [1-<sup>13</sup>C]Fumarate
Ausgabe der Zeitschrift
141

Datenquelle: Crossref

Abstract
Hyperpolarized fumarate can be used as a probe of real-time metabolism in vivo, using carbon-13 magnetic resonance imaging. Dissolution dynamic nuclear polarization is commonly used to produce hyperpolarized fumarate, but a cheaper and faster alternative is to produce hyperpolarized fumarate via PHIP (parahydrogen-induced polarization). In this work, we trans-hydrogenate [1-<sup>13</sup>C]acetylene dicarboxylate with para-enriched hydrogen using a commercially available Ru catalyst in water to produce hyperpolarized [1-<sup>13</sup>C]fumarate. We show that fumarate is produced in 89% yield, with succinate as a side product in 11% yield. The proton polarization is converted into <sup>13</sup>C magnetization using a constant adiabaticity field cycle, and a polarization level of 24% is achieved using 86% para-enriched hydrogen gas. We inject the hyperpolarized [1-<sup>13</sup>C]fumarate into cell suspensions and track the metabolism. This work opens the path to greatly accelerated preclinical studies using fumarate as a biomarker.
Addresses
  • Helmholtz Institute , Johannes Gutenberg University of Mainz , Mainz 55099 , Germany.
Autoren
  • James Eills
  • Eleonora Cavallari
  • Carla Carrera
  • Dmitry Budker
  • Silvio Aime
  • Francesca Reineri
DOI
10.1021/jacs.9b10094
eISSN
1520-5126
Externe Identifier
  • PubMed Identifier: 31762271
Funding acknowledgements
  • Engineering and Physical Sciences Research Council: 1642101
  • Compagnia di San Paolo: CSTO164550
Open access
false
ISSN
0002-7863
Ausgabe der Veröffentlichung
51
Zeitschrift
Journal of the American Chemical Society
Schlüsselwörter
  • Carbon Isotopes
  • Fumarates
  • Fumarate Hydratase
  • Nuclear Magnetic Resonance, Biomolecular
  • Molecular Structure
  • Time Factors
Sprache
eng
Medium
Print-Electronic
Online publication date
2019
Paginierung
20209 - 20214
Datum der Veröffentlichung
2019
Status
Published
Datum der Datenerfassung
2019
Titel
Real-Time Nuclear Magnetic Resonance Detection of Fumarase Activity Using Parahydrogen-Hyperpolarized [1-<sup>13</sup>C]Fumarate.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
141

Datenquelle: Europe PubMed Central

Abstract
Hyperpolarized fumarate can be used as a probe of real-time metabolism in vivo, using carbon-13 magnetic resonance imaging. Dissolution dynamic nuclear polarization is commonly used to produce hyperpolarized fumarate, but a cheaper and faster alternative is to produce hyperpolarized fumarate via PHIP (parahydrogen-induced polarization). In this work, we trans-hydrogenate [1-13C]acetylene dicarboxylate with para-enriched hydrogen using a commercially available Ru catalyst in water to produce hyperpolarized [1-13C]fumarate. We show that fumarate is produced in 89% yield, with succinate as a side product in 11% yield. The proton polarization is converted into 13C magnetization using a constant adiabaticity field cycle, and a polarization level of 24% is achieved using 86% para-enriched hydrogen gas. We inject the hyperpolarized [1-13C]fumarate into cell suspensions and track the metabolism. This work opens the path to greatly accelerated preclinical studies using fumarate as a biomarker.
Autoren
  • James Eills
  • Eleonora Cavallari
  • Carla Carrera
  • Dmitry Budker
  • Silvio Aime
  • Francesca Reineri
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/31762271
DOI
10.1021/jacs.9b10094
eISSN
1520-5126
Ausgabe der Veröffentlichung
51
Zeitschrift
J Am Chem Soc
Schlüsselwörter
  • Carbon Isotopes
  • Fumarate Hydratase
  • Fumarates
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Time Factors
Sprache
eng
Country
United States
Paginierung
20209 - 20214
Datum der Veröffentlichung
2019
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Titel
Real-Time Nuclear Magnetic Resonance Detection of Fumarase Activity Using Parahydrogen-Hyperpolarized [1-13C]Fumarate.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
141

Datenquelle: PubMed

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