Impact of Branching on the Solution Behavior and Serum Stability of Starlike Block Copolymers

Autoren

Regina Holm
Marcel Douverne
Benjamin Weber
Tobias Bauer
Andreas Best
Patrick Ahlers
Kaloian Koynov
Pol Besenius
Matthias Barz

DOI

10.1021/acs.biomac.8b01545

eISSN

1526-4602

ISSN

1525-7797

Ausgabe der Veröffentlichung

1

Zeitschrift

Biomacromolecules

Sprache

en

Online publication date

2018

Paginierung

375 - 388

Datum der Veröffentlichung

2019

Status

Published

Herausgeber

American Chemical Society (ACS)

Herausgeber URL

http://dx.doi.org/10.1021/acs.biomac.8b01545

Datum der Datenerfassung

2023

Titel

Impact of Branching on the Solution Behavior and Serum Stability of Starlike Block Copolymers

Ausgabe der Zeitschrift

20

Datenquelle: Crossref

Abstract

The size control of nanomedicines for tumor diagnosis and therapy is of high importance, since it enables or disables deep and sufficient tumor penetration. Amphiphilic star-shaped block copolypept(o)ides offer substantial promise to precisely adjust the hydrophobic core and the hydrophilic corona, independent of each other, and therefore simultaneously control the size dimension in the interesting size range from 10 to 30 nm. To gain access to core-shell structures of such sizes, 3-arm and 6-arm PeptoStars, based on poly(γ- tert-butyloxycarbonyl-l-glutamate)- b-polysarcosine (pGlu(O tBu)- b-pSar), were prepared via controlled living ring-opening polymerization (ROP) of the corresponding N-carboxyanhydrides. Moreover, size exclusion chromatography (SEC) proves the presence of well-defined star shaped polymers with molecular weights from 38 to 88 kg/mol with low polymer dispersities of 1.16 to 1.23. By varying the α-helical peptide core and maintain a constant polysarcosine corona, hydrodynamic size analyses revealed the importance of using a sufficiently large and dense hydrophilic shielding corona to prevent aggregation of the hydrophobic core and obtain uniform-sized spherical-shaped particles with hydrodynamic diameters below 24 nm. Fluorescence correlation spectroscopy (FCS) additionally demonstrates the absence of protein adsorption in human plasma for 6-arm polypept(o)ide stars and thus confirms polysarcosine as stealthlike material.

Addresses

Institute of Organic Chemistry , Johannes Gutenberg-Universität Mainz , Duesbergweg 10-14 , 55128 Mainz , Germany.

Autoren

Regina Holm
Marcel Douverne
Benjamin Weber
Tobias Bauer
Andreas Best
Patrick Ahlers
Kaloian Koynov
Pol Besenius
Matthias Barz

DOI

10.1021/acs.biomac.8b01545

eISSN

1526-4602

Externe Identifier

PubMed Identifier: 30475598

Funding acknowledgements

Deutsche Forschungsgemeinschaft: SFB-1066-1/2

Open access

false

ISSN

1525-7797

Ausgabe der Veröffentlichung

1

Zeitschrift

Biomacromolecules

Schlüsselwörter

Plasma
Humans
Sarcosine
Peptides
Oligopeptides
Nanoparticles
Polymerization
Protein Corona
Protein Conformation, alpha-Helical

Sprache

eng

Medium

Print-Electronic

Online publication date

2018

Paginierung

375 - 388

Datum der Veröffentlichung

2019

Status

Published

Datum der Datenerfassung

2018

Titel

Impact of Branching on the Solution Behavior and Serum Stability of Starlike Block Copolymers.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

20

Datenquelle: Europe PubMed Central

Abstract

The size control of nanomedicines for tumor diagnosis and therapy is of high importance, since it enables or disables deep and sufficient tumor penetration. Amphiphilic star-shaped block copolypept(o)ides offer substantial promise to precisely adjust the hydrophobic core and the hydrophilic corona, independent of each other, and therefore simultaneously control the size dimension in the interesting size range from 10 to 30 nm. To gain access to core-shell structures of such sizes, 3-arm and 6-arm PeptoStars, based on poly(γ- tert-butyloxycarbonyl-l-glutamate)- b-polysarcosine (pGlu(O tBu)- b-pSar), were prepared via controlled living ring-opening polymerization (ROP) of the corresponding N-carboxyanhydrides. Moreover, size exclusion chromatography (SEC) proves the presence of well-defined star shaped polymers with molecular weights from 38 to 88 kg/mol with low polymer dispersities of 1.16 to 1.23. By varying the α-helical peptide core and maintain a constant polysarcosine corona, hydrodynamic size analyses revealed the importance of using a sufficiently large and dense hydrophilic shielding corona to prevent aggregation of the hydrophobic core and obtain uniform-sized spherical-shaped particles with hydrodynamic diameters below 24 nm. Fluorescence correlation spectroscopy (FCS) additionally demonstrates the absence of protein adsorption in human plasma for 6-arm polypept(o)ide stars and thus confirms polysarcosine as stealthlike material.

Autoren

Regina Holm
Marcel Douverne
Benjamin Weber
Tobias Bauer
Andreas Best
Patrick Ahlers
Kaloian Koynov
Pol Besenius
Matthias Barz

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/30475598

DOI

10.1021/acs.biomac.8b01545

eISSN

1526-4602

Ausgabe der Veröffentlichung

1

Zeitschrift

Biomacromolecules

Schlüsselwörter

Humans
Nanoparticles
Oligopeptides
Peptides
Plasma
Polymerization
Protein Conformation, alpha-Helical
Protein Corona
Sarcosine

Sprache

eng

Country

United States

Paginierung

375 - 388

Datum der Veröffentlichung

2019

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2020

Titel

Impact of Branching on the Solution Behavior and Serum Stability of Starlike Block Copolymers.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

20

Datenquelle: PubMed

Impact of Branching on the Solution Behavior and Serum Stability of Starlike Block Copolymers

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