Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins
- Publikationstyp:
- Kapitel
- Metadaten:
-
- Autoren
- Eszter Kozma
- Giulia Paci
- Gemma Estrada Girona
- Edward A Lemke
- Peter Kele
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000434794700023&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1007/978-1-4939-7574-7_22
- ISBN-13
- 978-1-4939-7573-0
- Schlüsselwörter
- Bioorthogonality
- Tetrazine
- Fluorogenicity
- NIR emission
- Super-resolution microscopy
- Paginierung
- 337 - 363
- Buchtitel
- NONCANONICAL AMINO ACIDS: METHODS AND PROTOCOLS
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Titel
- Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins
- Ausgabe der Zeitschrift
- 1728
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Eszter Kozma
- Giulia Paci
- Gemma Estrada Girona
- Edward A Lemke
- Péter Kele
- DOI
- 10.1007/978-1-4939-7574-7_22
- ISBN-13
- 9781493975730
- Online publication date
- 2018
- Paginierung
- 337 - 363
- Buchtitel
- Methods in Molecular Biology
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Herausgeber
- Springer New York
- Herausgeber URL
- http://dx.doi.org/10.1007/978-1-4939-7574-7_22
- Datum der Datenerfassung
- 2019
- Titel
- Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins
Datenquelle: Crossref
- Abstract
- Tetrazine-bearing fluorescent labels enable site-specific tagging of proteins that are genetically manipulated with dienophile modified noncanonical amino acids. The inverse electron demand Diels-Alder reaction between the tetrazine and the dienophile fulfills the criteria of bioorthogonality allowing fluorescent labeling schemes of live cells. Here, we describe the detailed synthetic and labeling protocols of a near infrared emitting siliconrhodamine-tetrazine probe suitable for super-resolution imaging of residue-specifically engineered proteins in mammalian cells.
- Addresses
- Chemical Biology Research Group, Research Centre for Natural Sciences, Institute of Organic Chemistry, Hungarian Academy of Sciences, Magyar tudósok krt 2, Budapest, 1117, Hungary.
- Autoren
- Eszter Kozma
- Giulia Paci
- Gemma Estrada Girona
- Edward A Lemke
- Péter Kele
- DOI
- 10.1007/978-1-4939-7574-7_22
- Open access
- false
- Schlüsselwörter
- COS Cells
- Animals
- Humans
- Silicon
- Rhodamines
- Amino Acids
- Proteins
- Fluorescent Dyes
- Microscopy, Fluorescence
- Staining and Labeling
- Transfection
- Chlorocebus aethiops
- Medium
- Paginierung
- 337 - 363
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Datum der Datenerfassung
- 2018
- Titel
- Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins.
- Ausgabe der Zeitschrift
- 1728
Datenquelle: Europe PubMed Central
- Abstract
- Tetrazine-bearing fluorescent labels enable site-specific tagging of proteins that are genetically manipulated with dienophile modified noncanonical amino acids. The inverse electron demand Diels-Alder reaction between the tetrazine and the dienophile fulfills the criteria of bioorthogonality allowing fluorescent labeling schemes of live cells. Here, we describe the detailed synthetic and labeling protocols of a near infrared emitting siliconrhodamine-tetrazine probe suitable for super-resolution imaging of residue-specifically engineered proteins in mammalian cells.
- Autoren
- Eszter Kozma
- Giulia Paci
- Gemma Estrada Girona
- Edward A Lemke
- Péter Kele
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/29405009
- DOI
- 10.1007/978-1-4939-7574-7_22
- Schlüsselwörter
- Bioorthogonality
- Fluorogenicity
- NIR emission
- Super-resolution microscopy
- Tetrazine
- Amino Acids
- Animals
- COS Cells
- Chlorocebus aethiops
- Fluorescent Dyes
- Humans
- Microscopy, Fluorescence
- Proteins
- Rhodamines
- Silicon
- Staining and Labeling
- Transfection
- Paginierung
- 337 - 363
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2018
- Titel
- Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins.
- Ausgabe der Zeitschrift
- 1728
Datenquelle: PubMed
- Beziehungen:
- Eigentum von