Infection with adeno-associated virus may protect against excitotoxicity

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • EB Dreyer
  • CK Vorwerk
  • D Zurakowski
  • PD Simon
  • J Bennett
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000082865500005&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1097/00001756-199909290-00002
Externe Identifier
  • Clarivate Analytics Document Solution ID: 241CV
  • PubMed Identifier: 10549791
ISSN
0959-4965
Ausgabe der Veröffentlichung
14
Zeitschrift
NEUROREPORT
Schlüsselwörter
  • adeno-associated virus
  • excitotoxicity
  • glutamate
Paginierung
2887 - 2890
Datum der Veröffentlichung
1999
Status
Published
Titel
Infection with adeno-associated virus may protect against excitotoxicity
Sub types
  • Article
Ausgabe der Zeitschrift
10

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Autoren
  • Evan B Dreyer
  • Christian K Vorwerk
  • David Zurakowski
  • Perikles D Simon
  • Jean Bennett
DOI
10.1097/00001756-199909290-00002
ISSN
0959-4965
Ausgabe der Veröffentlichung
14
Zeitschrift
NeuroReport
Sprache
en
Paginierung
2887 - 2890
Datum der Veröffentlichung
1999
Status
Published
Herausgeber
Ovid Technologies (Wolters Kluwer Health)
Herausgeber URL
http://dx.doi.org/10.1097/00001756-199909290-00002
Datum der Datenerfassung
2021
Titel
Infection with adeno-associated virus may protect against excitotoxicity
Ausgabe der Zeitschrift
10

Datenquelle: Crossref

Abstract
Gene therapy has developed as a promising approach for therapy in a broad variety of conditions. Viral vectors have been developed that may replace a defective gene, prevent expression of a mutant gene, or deliver a protective gene and thereby delay cellular loss. Using adeno-associated virus containing green fluorescent protein (AAV-GFP) we were able to specifically transduce cells located in the inner retina and induce over-expression of GFP in adult rat retinae. The delivery and expression of GFP had no influence themselves on retinal ganglion cell survival. Administration of the reporter vector AAV-GFP provided retinal ganglion cells with slight but significant protection from intravitreal NMDA. This was a locally mediated phenomenon; greater protection was seen in regions with more transduced cells. Any evaluation of the efficacy of a putative viral vector should consider the possible protective or toxic effect of the native virus.
Addresses
  • Department of Ophthalmology, University of Pennsylvania, Scheie Eye Institute, Philadelphia 19104, USA.
Autoren
  • EB Dreyer
  • CK Vorwerk
  • D Zurakowski
  • PD Simon
  • J Bennett
DOI
10.1097/00001756-199909290-00002
eISSN
1473-558X
Externe Identifier
  • PubMed Identifier: 10549791
Funding acknowledgements
  • NEI NIH HHS: R01 EY10009
Open access
false
ISSN
0959-4965
Ausgabe der Veröffentlichung
14
Zeitschrift
Neuroreport
Schlüsselwörter
  • Retinal Ganglion Cells
  • Animals
  • Rats
  • Rats, Sprague-Dawley
  • Dependovirus
  • N-Methylaspartate
  • Luminescent Proteins
  • Green Fluorescent Proteins
  • Excitatory Amino Acid Agonists
  • Transduction, Genetic
  • Cell Survival
  • Genes, Reporter
  • Genetic Vectors
Sprache
eng
Medium
Print
Paginierung
2887 - 2890
Datum der Veröffentlichung
1999
Status
Published
Datum der Datenerfassung
1999
Titel
Infection with adeno-associated virus may protect against excitotoxicity.
Sub types
  • Research Support, U.S. Gov't, P.H.S.
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
10

Datenquelle: Europe PubMed Central

Abstract
Gene therapy has developed as a promising approach for therapy in a broad variety of conditions. Viral vectors have been developed that may replace a defective gene, prevent expression of a mutant gene, or deliver a protective gene and thereby delay cellular loss. Using adeno-associated virus containing green fluorescent protein (AAV-GFP) we were able to specifically transduce cells located in the inner retina and induce over-expression of GFP in adult rat retinae. The delivery and expression of GFP had no influence themselves on retinal ganglion cell survival. Administration of the reporter vector AAV-GFP provided retinal ganglion cells with slight but significant protection from intravitreal NMDA. This was a locally mediated phenomenon; greater protection was seen in regions with more transduced cells. Any evaluation of the efficacy of a putative viral vector should consider the possible protective or toxic effect of the native virus.
Autoren
  • EB Dreyer
  • CK Vorwerk
  • D Zurakowski
  • PD Simon
  • J Bennett
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/10549791
DOI
10.1097/00001756-199909290-00002
Funding acknowledgements
  • NEI NIH HHS: R01 EY10009
ISSN
0959-4965
Ausgabe der Veröffentlichung
14
Zeitschrift
Neuroreport
Schlüsselwörter
  • Animals
  • Cell Survival
  • Dependovirus
  • Excitatory Amino Acid Agonists
  • Genes, Reporter
  • Genetic Vectors
  • Green Fluorescent Proteins
  • Luminescent Proteins
  • N-Methylaspartate
  • Rats
  • Rats, Sprague-Dawley
  • Retinal Ganglion Cells
  • Transduction, Genetic
Sprache
eng
Country
England
Paginierung
2887 - 2890
Datum der Veröffentlichung
1999
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2000
Titel
Infection with adeno-associated virus may protect against excitotoxicity.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
Ausgabe der Zeitschrift
10

Datenquelle: PubMed

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