WHO grade associated downregulation of MHC class I antigen-processing machinery components in human astrocytomas: does it reflect a potential immune escape mechanism?
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Matthias Mehling
- Perikles Simon
- Michel Mittelbronn
- Richard Meyermann
- Soldano Ferrone
- Michael Weller
- Heinz Wiendl
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000248046800002&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1007/s00401-007-0231-8
- Externe Identifier
- Clarivate Analytics Document Solution ID: 190GK
- PubMed Identifier: 17541610
- ISSN
- 0001-6322
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- ACTA NEUROPATHOLOGICA
- Schlüsselwörter
- MHC
- antigen-processing machinery
- astrocytoma
- immune escape
- Paginierung
- 111 - 119
- Datum der Veröffentlichung
- 2007
- Status
- Published
- Titel
- WHO grade associated downregulation of MHC class I antigen-processing machinery components in human astrocytomas: does it reflect a potential immune escape mechanism?
- Sub types
- Article
- Ausgabe der Zeitschrift
- 114
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Matthias Mehling
- Perikles Simon
- Michel Mittelbronn
- Richard Meyermann
- Soldano Ferrone
- Michael Weller
- Heinz Wiendl
- DOI
- 10.1007/s00401-007-0231-8
- eISSN
- 1432-0533
- ISSN
- 0001-6322
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Acta Neuropathologica
- Sprache
- en
- Online publication date
- 2007
- Paginierung
- 111 - 119
- Datum der Veröffentlichung
- 2007
- Status
- Published
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1007/s00401-007-0231-8
- Datum der Datenerfassung
- 2023
- Titel
- WHO grade associated downregulation of MHC class I antigen-processing machinery components in human astrocytomas: does it reflect a potential immune escape mechanism?
- Ausgabe der Zeitschrift
- 114
Datenquelle: Crossref
- Abstract
- Defects of major histocompatibility complex (MHC) class I antigen-processing machinery (APM) components have been shown to contribute to immune escape of malignant cells. We investigated the expression of APM components in astrocytomas without detectable defects in HLA class I antigen expression and correlated it with grade of malignancy. Quantitative immunohistochemical analysis of astrocytomas revealed reduced expression of the cytosolic proteasome subunit low molecular weight protein 2 (LMP2), the endoplasmatic reticulum (ER) transporter associated with antigen processing-1 (TAP1), and the ER chaperone beta2-microglobulin (beta2m) in astrocytoma cells when compared to astrocytes from nonpathological brain. Among human WHO grade II-IV astrocytomas, downregulation of LMP2, TAP1 and beta2m correlated with grade of malignancy. Furthermore, astrocytoma cell lines (n = 12) expressed all APM components analyzed at levels comparable to dendritic cells (DC), which were used for comparative purposes. However, upregulation of beta2m after stimulation with inflammatory cytokines was significantly lower in astrocytoma cell lines than in control cells. Our results support the hypothesis that coordinated downregulation or impaired upregulation of certain HLA class I APM components may serve as a mechanism for astrocytoma cells to evade the host's immune response, even if HLA class I antigen surface expression is not altered.
- Addresses
- Department of General Neurology, Hertie-Institute for Clinical Brain Research, University of Tübingen, Hoppe-Seyler-Strasse 3, 72076, Tübingen, Germany.
- Autoren
- Matthias Mehling
- Perikles Simon
- Michel Mittelbronn
- Richard Meyermann
- Soldano Ferrone
- Michael Weller
- Heinz Wiendl
- DOI
- 10.1007/s00401-007-0231-8
- eISSN
- 1432-0533
- Externe Identifier
- PubMed Identifier: 17541610
- Open access
- false
- ISSN
- 0001-6322
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Acta neuropathologica
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Astrocytoma
- Brain Neoplasms
- Cysteine Endopeptidases
- beta 2-Microglobulin
- ATP-Binding Cassette Transporters
- Histocompatibility Antigens Class I
- Flow Cytometry
- Immunohistochemistry
- Antigen Presentation
- Tumor Escape
- Gene Expression
- Down-Regulation
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Middle Aged
- Child
- Child, Preschool
- World Health Organization
- Female
- Male
- ATP Binding Cassette Transporter, Subfamily B, Member 2
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2007
- Paginierung
- 111 - 119
- Datum der Veröffentlichung
- 2007
- Status
- Published
- Datum der Datenerfassung
- 2007
- Titel
- WHO grade associated downregulation of MHC class I antigen-processing machinery components in human astrocytomas: does it reflect a potential immune escape mechanism?
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 114
Datenquelle: Europe PubMed Central
- Abstract
- Defects of major histocompatibility complex (MHC) class I antigen-processing machinery (APM) components have been shown to contribute to immune escape of malignant cells. We investigated the expression of APM components in astrocytomas without detectable defects in HLA class I antigen expression and correlated it with grade of malignancy. Quantitative immunohistochemical analysis of astrocytomas revealed reduced expression of the cytosolic proteasome subunit low molecular weight protein 2 (LMP2), the endoplasmatic reticulum (ER) transporter associated with antigen processing-1 (TAP1), and the ER chaperone beta2-microglobulin (beta2m) in astrocytoma cells when compared to astrocytes from nonpathological brain. Among human WHO grade II-IV astrocytomas, downregulation of LMP2, TAP1 and beta2m correlated with grade of malignancy. Furthermore, astrocytoma cell lines (n = 12) expressed all APM components analyzed at levels comparable to dendritic cells (DC), which were used for comparative purposes. However, upregulation of beta2m after stimulation with inflammatory cytokines was significantly lower in astrocytoma cell lines than in control cells. Our results support the hypothesis that coordinated downregulation or impaired upregulation of certain HLA class I APM components may serve as a mechanism for astrocytoma cells to evade the host's immune response, even if HLA class I antigen surface expression is not altered.
- Date of acceptance
- 2007
- Autoren
- Matthias Mehling
- Perikles Simon
- Michel Mittelbronn
- Richard Meyermann
- Soldano Ferrone
- Michael Weller
- Heinz Wiendl
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/17541610
- DOI
- 10.1007/s00401-007-0231-8
- ISSN
- 0001-6322
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Acta Neuropathol
- Schlüsselwörter
- ATP Binding Cassette Transporter, Subfamily B, Member 2
- ATP-Binding Cassette Transporters
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Antigen Presentation
- Astrocytoma
- Brain Neoplasms
- Cell Line, Tumor
- Child
- Child, Preschool
- Cysteine Endopeptidases
- Down-Regulation
- Female
- Flow Cytometry
- Gene Expression
- Histocompatibility Antigens Class I
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Tumor Escape
- World Health Organization
- beta 2-Microglobulin
- Sprache
- eng
- Country
- Germany
- Paginierung
- 111 - 119
- Datum der Veröffentlichung
- 2007
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2007
- Titel
- WHO grade associated downregulation of MHC class I antigen-processing machinery components in human astrocytomas: does it reflect a potential immune escape mechanism?
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 114
Datenquelle: PubMed
- Beziehungen:
- Eigentum von